EP0463508A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for injecting substances into cells
Assignee: WR Grace and Co Conn
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Filing: 1991-06-14
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Granted: —
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Expires: 2011-06-14
Abstract
Abstract The present invention provides a method for introducing a substance into a cell on a plate capable of immobilizing and easily detaching the cells. More specifically, the method employs the use of a temperature-responsive polymeric compound and a cell adhesive substance to immobilize and detach the cells.
Inventors
- 👤 Yuichi Mori
- 👤 Manabu Yamazaki
- 👤 Toshiaki Takezawa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 14:57
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/87; C12N15/89; C12N5/00; C12N5/0068; C12N2539/00; C12N2539/10
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
90 words
A method for injecting a substance into a cell which comprises the steps of: (i) immobilizing a cell on a plate comprising (a) a temperature-responsive polymeric compound having an LCST lower than the injection temperature and (b) a cell adhesive substance, at a temperature higher than said LCST, (ii) injecting a substance into the cell immobilized on said plate at a temperature higher than said LCST, and (iii) lowering said temperature to a temperature below said LCST to detach and recover the cell injected with said substance from said plate.
Claim 2
Dependent Claim
12 words
The method of Claim 1 wherein said substance is injected by microinjection.
Claim 3
Dependent Claim
35 words
The method of Claim 1, wherein said temperature- responsive polymeric compound is selected from a group consisting of poly-N-substituted acrylamide derivatives, poly-N-substituted methacrylamide derivatives or their copolymers, polyvinyl methyl ethers and partially acetylated polyvinyl alcohols.
Claim 4
Dependent Claim
35 words
The method of Claim 1, wherein said cell adhesive substance is selected from a group consisting of extracellular matrix components, gelatin, lectins, anchorage oligopeptides, adhesive proteins isolated from shellfish, positively charged polymers and their mixtures.
Claim 5
Dependent Claim
25 words
The method of Claim 4, wherein said extracellular matrix component is selected from the group consisting of collagen, fibronectin, vitronectin, laminin, proteoglycan, glycosaminoglycan and thrombospondin.
Claim 6
Dependent Claim
12 words
The method of Claim 1, wherein said cell is a mammalian cell.
Claim 7
Dependent Claim
24 words
The method of Claim 6 wherein said mammalian cell is selected from the group consisting of tissue cells, organ cells, blood cells and oocytes.
Claim 8
Dependent Claim
24 words
The method of Claim 1, wherein said substance is selected from the group consisting of genes, embryos, proteins, mRNAs, plasmid vectors, enzymes and viruses.
References Cited (Backward Citations)
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