EP0350714A2
Expired / Lapsed
EP
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Tissue immobilization and cell culturing system and method for affixing biologically active moieties to a substrate
Assignee: Discovery Labware Inc
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Filing: 1989-06-28
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Granted: —
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Expires: 2009-06-28
Abstract
Abstract A cell culturing system, methods for the preparation thereof, and methods for affixing other biologically active moieties to a substrate are provided. Said cell culturing system comprises: a substrate; a film comprising polyallylamine on said substrate; viable cells affixed to said substrate; and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.
Inventors
- 👤 Nishith Chaturvedi
- 👤 Paul T. Picciano
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 04:24
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61L; A61L27/00; A61L27/36; A61L27/38; C12N2533/00; C12N2533/30
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
59 words
Method for affixing biologically active moieties to a substrate comprising: (1) applying a film-forming polyallylamine composition comprising from about 0.01 to about 1.0% by weight of polyallylamine to a substrate; (2) removing extraneous materials not firmly attached to said substrate; and (3) applying biologically active moieties to said substrate, whereby said biologically active moieties become affixed to said substrate.
Claim 2
Dependent Claim
59 words
Method for affixing biologically active moieties to a substrate comprising: (1) applying a film-forming polyallylamine composition comprising from about 0.01 to about 1.0% by weight of polyallylamine to a substrate; (2) removing extraneous materials not firmly attached to said substrate; and (3) applying biologically active moieties to said substrate, whereby said biologically active moieties become affixed to said substrate.
Claim 3
Dependent Claim
37 words
Method as defined in Claim 1 wherein from about 1.0 to about 100 µl of said film-forming composition containing from about 0.1 to about 10 µg/µl of polyallylamine per cm² of substrate is applied to said substrate.
Claim 4
Dependent Claim
27 words
Method as defined in Claim 1 wherein said film-forming composition is acidic and the portion thereof not firmly attached to said substrate is evaporated from said substrate.
Claim 5
Dependent Claim
33 words
Method as defined in Claim 1 wherein said film-forming composition is neutral or basic and, after an incubation period, the portion thereof not firmly attached to said substrate can be removed by aspiration.
Claim 6
Dependent Claim
12 words
Method as defined in Claim 1 wherein said film-forming composition is sterile.
Claim 7
Dependent Claim
21 words
Method as defined in Claim 1 wherein said substrate is rinsed to remove extraneous materials with water or a buffered solution.
Claim 8
Dependent Claim
24 words
Method as defined in Claim 1 wherein said substrate is selected from the group consisting of synthetic polymeric materials, glass, metals, and microporous filters.
Claim 9
Dependent Claim
11 words
Method as defined in Claim 7 wherein said substrate is polytetrafluoroethylene.
Claim 10
Dependent Claim
29 words
Method as defined in Claim 1 wherein the biologically active moieties are selected from the group consisting of tissue sections, cells, cellular components, serum and other body fluid components.
Claim 11
Dependent Claim
66 words
Method of affixing viable cells to a substrate comprising: (1) applying a film-forming polyallylamine composition comprising from about 0.01 to about 1.0% by weight of polyallylamine to a substrate; (2) removing extraneous materials not firmly attached to said substrate; (3) applying viable cells to said substrate, whereby said cells become affixed to said substrate and, in the presence of a nutritive environment, perform normal metalbolic functions.
Claim 12
Dependent Claim
66 words
Method of affixing viable cells to a substrate comprising: (1) applying a film-forming polyallylamine composition comprising from about 0.01 to about 1.0% by weight of polyallylamine to a substrate; (2) removing extraneous materials not firmly attached to said substrate; (3) applying viable cells to said substrate, whereby said cells become affixed to said substrate and, in the presence of a nutritive environment, perform normal metalbolic functions.
Claim 13
Dependent Claim
38 words
Method as defined in Claim 10 wherein from about 1.0 to about 100 µl of said film-forming composition containing from about 0.1 to about 10 µg/ µl of polyallylamine per cm² of substrate is applied to said substrate.
Claim 14
Dependent Claim
27 words
Method as defined in Claim 10 wherein said film-forming composition is acidic and the portion thereof not firmly attached to said substrate is evaporated from said substrate.
Claim 15
Dependent Claim
33 words
Method as defined in Claim 10 wherein said film-forming composition is neutral or basic and, after an incubation period, the portion thereof not firmly attached to said substrate can be removed by aspiration.
Claim 16
Dependent Claim
12 words
Method as defined in Claim 10 wherein said film-forming composition is sterile.
Claim 17
Dependent Claim
22 words
Method as defined in Claim 10 wherein said substrate is rinsed to remove extraneous materials with sterile aqueous serum-free tissue culture medium.
Claim 18
Dependent Claim
20 words
Method as defined in Claim 10 wherein said substate is rinsed to remove extraneous materials with water or buffered solution.
Claim 19
Dependent Claim
19 words
Method as defined in Claim 10 wherein the viable cells are applied to said substrate in a serum-containing medium.
Claim 20
Dependent Claim
19 words
Method as defined in Claim 10 wherein the viable cells are applied to said substrate in a serum-free medium.
Claim 21
Dependent Claim
14 words
Method as defined in Claim 10 wherein said substrate is an in vitro substrate.
Claim 22
Dependent Claim
24 words
Method as defined in Claim 19 wherein said substrate is selected from the group consisting of synthetic polymeric materials, glass, metals, and microporous filters.
Claim 23
Dependent Claim
11 words
Method as defined in Claim 20 wherein said substrate is polytetrafluoroethylene.
Claim 24
Dependent Claim
14 words
Method as defined in Claim 10 wherein said substrate is an in vivo substrate.
Claim 25
Dependent Claim
24 words
Method as defined in Claim 22 wherein the substrate is selected from the group consisting of collagen, laminin, fibronectin, prosthetic grafting materials and polylysine.
Claim 26
Dependent Claim
11 words
Method as defined in Claim 23 wherein said substrate is polytetrafluoroethylene.
Claim 27
Dependent Claim
36 words
A cell culturing system comprising a substrate, a film comprising polyallylamine on said substrate, viable cells afixed to said film-containing substrate, and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.
Claim 28
Dependent Claim
36 words
A cell culturing system comprising a substrate, a film comprising polyallylamine on said substrate, viable cells afixed to said film-containing substrate, and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.
Claim 29
Dependent Claim
17 words
A cell culturing system as defined in Claim 25 wherein the substrate is an in vitro substrate.
Claim 30
Dependent Claim
27 words
A cell culturing system as defined in Claim 26 wherein the subtrate is selected from the group consisting of synthetic polymeric materials, glass, metals, and microporous filters.
Claim 31
Dependent Claim
14 words
A cell culturing system as defined in Claim 27 wherein said substrate is polytetrafluoroethylene.
Claim 32
Dependent Claim
17 words
A cell culturing system as defined in Claim 25 wherein said substrate is an in vivo substrate.
Claim 33
Dependent Claim
27 words
A cell culturing system as defined in Claim 29 wherein the substrate is selected from the group consisting of collagen, laminin, fibronectin, prosthetic grafting materials and polylysine
Claim 34
Dependent Claim
71 words
Method of affixing biologically active moieties to a substrate, comprising: (1) dispersing said biologically active moieties in a serum free solution; (2) admixing a formulation comprising polyallylamine comprising from about 0.01 to about 1.0% by weight polyallylamine with said dispersion of biologically active moieties, thereby attaching said polyallylamine to said biologically active moieties; (3) recovering the resulting biologically active moieties; and (4) affixing said recovered biologically active moieties to said substrate.
Claim 35
Dependent Claim
71 words
Method of affixing biologically active moieties to a substrate, comprising: (1) dispersing said biologically active moieties in a serum free solution; (2) admixing a formulation comprising polyallylamine comprising from about 0.01 to about 1.0% by weight polyallylamine with said dispersion of biologically active moieties, thereby attaching said polyallylamine to said biologically active moieties; (3) recovering the resulting biologically active moieties; and (4) affixing said recovered biologically active moieties to said substrate.
References Cited (Backward Citations)
No citation records indexed.
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