Patent EP0458367B1 successfully refreshed from Google Patents.
EP0458367B1
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EP
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Anti-sense regulation of gene expression in plant cells
Assignee: Monsanto Co
•
Filing: 1987-03-19
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Granted: 2001-10-17
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Expires: 2007-03-19
Abstract
No abstract record available.
Inventors
- 👤 Christine K. Shewmaker
- 👤 Jean C. Kridl
- 👤 William R. Hiatt
- 👤 Vic Knauf
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 14:04
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1137; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8218; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12Y; C12Y302/00; C12Y302/01; C12Y302/01015; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; Y; Y02; Y02A; Y02A40/00; Y02A40/10; Y02A40/146
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
126 words
A method for regulating the expression of a gene in a
plant cell which comprises: integrating into said plant cell genome a construct
comprising a promoter functional in said plant cell, a dsDNA
sequence having the transcribed strand complementary to RNA
endogenous in said cell and a termination region functional
in said cell; growing said plant cell containing said integrated
construct, whereby said complementary strand is transcribed
and modulates the function of said endogenous RNA in said
cell, provided: (i) that the gene is not a plant parasite gene; and (ii) that said dsDNA sequence is not a
polygalacturonase (PG) DNA sequence as found in
the EcoRI insert of plasmid pCGN1401 (ATCC67227),
or as obtainable by the use of said insert DNA as
a hybridization probe.
Claim 2
Dependent Claim
13 words
A method according to claim 1, wherein said endogenous
RNA is messenger RNA.
Claim 3
Dependent Claim
17 words
A method according to claim 2, wherein modulation of
said function results in an altered phenotypic trait.
Claim 4
Dependent Claim
25 words
A method according to claim 2 or 3 wherein the
transcribed strand includes at least 15 nt of the translated
region of said messenger RNA.
Claim 5
Dependent Claim
26 words
A method according to claim 2 or 3 wherein the
transcribed strand includes at least 15 nt of the 5'
untranslated sequence of said messenger RNA.
Claim 6
Dependent Claim
26 words
A method according to claim 2 or 3 wherein the
transcribed strand includes at least 15 nt of the 3'
untranslated sequence of said messenger RNA.
Claim 7
Independent Claim
22 words
A method according to any one of claims 2 to 6, wherein
modulation of said function results in an altered phenotypic
trait.
References Cited (Backward Citations)
No citation records indexed.
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