Method for specific amplification of RNA templates and for the determination of RNA

Abstract

Abstract A method for the specific amplification and for the detection of nucleic acid templates by hybridisation of two nucleic acid primers onto this template, formation of a template-complementary nucleic acid with these primers and transcription of the nucleic acid which is formed to give a plurality of template-analogous nucleic acids, characterised in that the template is hybridised with two primers which are arranged in the same orientation, contain sequences complementary to the template and of which the second primer carries at the end opposite to the first second primer a transcription initiation site and a double-stranded sequence to which an RNA polymerase can bind, the two primers hybridised in this way are covalently linked by filling in the gap between them to give a template-complementary nucleic acid, transcripts of this template-complementary nucleic acid are formed, and these transcripts are detected where appropriate in a manner known per se.

Inventors

• 👤 Christoph Dr.Rer.Nat. Kessler
• 👤 Rüdiger Dr.med. Rüger

Bibliographic Details