Patent EP0446450A1 successfully refreshed from Google Patents.
EP0446450A1
Expired / Lapsed
EP
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Device for collecting and processing fat tissue to produce endothelial cell product
Assignee: Thomas Jefferson University
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Filing: 1990-12-13
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Granted: —
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Expires: 2010-12-13
Abstract
Abstract The invention is an endothelial cell procurement and deposition kit for collecting fat from a patient, processing said fat to produce an endothelial cell deposition product, and depositing said product on the surface of a graft, all under sterile conditions established and maintained within the components of said kit comprised of: fat collection means for collecting subcutaneous fat from a patient; digestion means connectable to said fat collection means to maintain sterility during reception of said fat and for retaining said fat under sterile conditions during rinsing and digestion to produce a digested product; endothelial cell isolation means connectable to said digestion means for maintaining sterile conditions during reception of said digested product and for separating and isolating microvessel endothelial cells from said digested product to produce an endothelial cell product; cell deposition means connectable to said isolation means for maintaining sterile conditions during reception of said endothelial cell product and for depositing said cells on the surface of a graft to be implanted in a patient and facilitating implantation of said endothelial graft into a patient. A single device for collecting and processing tissue to produce endothelial cell product is provided.
Inventors
- 👤 Paul G. Alchas
- 👤 Alfred W. Prais
- 👤 Bruce E. Jarrell
- 👤 Stuart K. Williams
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 05:28
CPC Classifications
A; A61; A61F; A61F2/00; A61F2/02; A61F2/04; A61F2/06; A61F2/062; C; C12; C12M; C12M45/00; C12M45/09; C12M47/00; C12M47/04; A61M; A61M2202/00; A61M2202/08; A61M2202/09; A61M2202/097
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
48 words
A device for collecting and processing tissue to produce an endothelial cell product characterized by a vessel having a digestion chamber, a waste chamber, and an isolation chamber which allows the digestion and processing of tissue to produce endothelial cell product within a single vessel under sterile conditions.
Claim 2
Dependent Claim
24 words
A device according to claim 1, characterized in that the digestion chamber is separated from the waste chamber by a normally closed check valve.
Claim 3
Dependent Claim
22 words
A device according to claim 2, characterized in that an internal venting means extends from said waste chamber into said isolation chamber.
Claim 4
Dependent Claim
29 words
A device according to claim 1, characterized in that the digestion chamber is separated from said isolation chamber by a screen for retaining undigested materials within said digestion chamber.
Claim 5
Dependent Claim
24 words
A device according to claim 1, characterized in that an ampule is disposed within said isolation chamber for receiving said isolated endothelial cell product.
Claim 6
Dependent Claim
81 words
A device according to claim 5, characterized in that a first two-position valve which when in a first open position allows communication between the upper and middle portions of said ampule and a second two-position valve which when in a first open position allows communication between the middle and lower positions of said ampule so that when each of said two-position valves are in a second closed position, said endothelial cell product is isolated within the middle portion of said ampule.
Claim 7
Dependent Claim
30 words
A device according to claim 1, characterized in that an inlet port is affixed to said vessel for introducing collected fat and rinse and enzyme solutions into said digestion chamber.
Claim 8
Independent Claim
53 words
A device for collecting and processing tissue to produce an endothelial cell product characterized by a vessel having an enzyme storage chamber, a waste chamber, a digestion chamber and a cell isolation chamber which allows the digestion and processing of tissue to produce endothelial cell product within a single vessel under sterile conditions.
Claim 9
Dependent Claim
49 words
A device according to claim 8, characterized in that the enzyme storage chamber communicates with said digestion chamber and the external environment, in that the waste chamber communicates with said digestion chamber, and in that the digestion chamber communicates with said enzyme storage chamber and said cell isolation chamber.
Claim 10
Dependent Claim
42 words
A device according to claim 9, characterized in that the communicating means between said enzyme storage chamber and the digestion chamber is a normally closed check valve which opens when pressure increases inside said enzyme storage chamber upon centrifugation of said device.
Claim 11
Dependent Claim
36 words
A device according to claim 9, characterized in that the communicating means between said digestion chamber and said cell isolation chamber is a direct connection having a screen to retain undigested materials within said digestion chamber.
Claim 12
Dependent Claim
39 words
A device according to claim 8, characterized in that the digestion chamber further comprises a means to introduce tissue to be digested within said chamber, and a means to introduce rinsing fluid into said chamber for rinsing said tissue.
Claim 13
Dependent Claim
27 words
A device according to claim 8, characterized in that the cell isolation chamber further comprises a means to isolate the endothelial cell product produced within said vessel.
Claim 14
Dependent Claim
39 words
A device according to claim 13, characterized in that the endothelial cell product isolating means comprises two two-position valves which when closed isolate the endothelial cell product in that portion of said cell isolation chamber between the two valves.
Claim 15
Dependent Claim
23 words
A device according claim 9, characterized in that an internal venting means is located between said enzyme storage chamber and said digestion chamber.
Claim 16
Independent Claim
139 words
A method of collecting and processing tissue to produce an endothelial cell product characterized by the steps of providing a device for collecting and processing tissue to produce an endothelial cell product comprising an enzyme storage chamber, a waste chamber, a digestion chamber, and a cell isolation chamber, introducing the tissue to be processed into said digestion chamber, introducing enzyme into said enzyme storage chamber, introducing a rinsing solution into said digestion chamber, agitating said device, centrifuging said device in one preferred direction thereby transferring said rinsing solution and waste materials into said waste chamber, centrifuging said device in a second preferred direction thereby transferring said enzyme from said enzyme storage chamber into said digestion chamber, agitating said device, and centrifuging said device in a second preferred direction to isolate the endothelial cell product within said cell isolation chamber.
References Cited (Backward Citations)
GB1356749A
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US4410630A
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