EP0441660A1
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EP
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Affinity separation with activated polyamide microporous membranes
Assignee: Akzo Nobel NV
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Filing: 1991-02-11
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Granted: —
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Expires: 2011-02-11
Abstract
Abstract Affinity membranes are prepared by activation of polyamide microporous films. In particular, the films are activated with 1,1′-carbonyldiimidazole, 2-fluoro-1-methyl-pyridinium (+) toluene-4-sulfonate (-) , trichlorotriazine, or derivatives thereof, followed by binding of ligand to the activated sites. The polyamide microporous activated films include composite films comprising polyamide covalently bound to a polyhydroxyl compound such as a polysaccharide.
Inventors
- 👤 Elias Klein
- 👤 Pamela A. Feldhoff
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 23:19
CPC Classifications
B; B01; B01D; B01D69/00; B01D69/12; B01D69/1214; B01D67/00; B01D67/0081; B01D67/0093; B01D67/00931; B01D69/14; B01D69/141; B01J; B01J20/00; B01J20/28; B01J20/28014; B01J20/28033; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3274; B01J20/328; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
30 words
A composite membrane for affinity separation comprising a polyamide microporous film covalently bonded to a polysaccharide, and further including a ligand adapted for affinity separation bound to the composite membrane.
Claim 2
Dependent Claim
17 words
The membrane of Claim 1, wherein the ligand is adapted for affinity purification of a biological fluid.
Claim 3
Dependent Claim
15 words
The composite membrane of Claim 1, wherein the polysaccharide is a polyglucose, dextran, or starch.
Claim 4
Dependent Claim
96 words
A method for the preparation of the composite membrane of Claim 1 for the immobilization of a ligand useful in an affinity separation or a compound useful in ion-exchange chromatography comprising: (1) activating at least some of the activation sites of a microporous polyamide film with an activator, (2) reacting the activated groups with a polyhydroxyl compound to covalently bind the polyhydroxyl compound to the polyamide film to form a composite matrix, and then (3) activating the unreacted activation sites of the composite matrix with a same or different activator to promote binding of the ligand.
Claim 5
Dependent Claim
12 words
The method of Claim 4, wherein the polyhydroxyl compound is a polysaccharide.
Claim 6
Dependent Claim
14 words
The method of Claim 4, wherein the polyhydroxyl compound is polyglucose, dextran, or starch.
Claim 7
Dependent Claim
45 words
A composite matrix for the immobilization of a ligand useful in an affinity separation or a compound useful in ion-exchange chromatography comprising a microporous polyamide film including activation sites activated with an activator and covalently bound to a polyhydroxyl compound to form the composite matrix.
Claim 8
Dependent Claim
14 words
The matrix of Claim 7, wherein the polyhydroxyl compound is an oligomer or polymer.
Claim 9
Dependent Claim
12 words
The matrix of Claim 7, wherein the polyhydroxyl compound is a polysaccharide.
Claim 10
Dependent Claim
18 words
The matrix of Claim 7, wherein the ligand is adapted for the affinity purification of a biological fluid.
References Cited (Backward Citations)
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