Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
77 words
A method for separating HDL from whole blood anticoagulated with EDTA which comprises mixing the whole blood with an aqueous solution composed of magnesium ⁺⁺ cations and dextran sulfate 500 in such proportions and of such concentration (a) that the LDL and VLDL lipoproteins and the chylomicrons are precipitated while (b) the HDL remains in solution, and sedimenting the precipitated lipoproteins, the chylomicrons and the red bloods cells by centrifuging at not more than 1,000 X gravity.
Claim 2
Independent Claim
77 words
A method for separating HDL from whole blood anticoagulated with EDTA which comprises mixing the whole blood with an aqueous solution composed of magnesium ⁺⁺ cations and dextran sulfate 500 in such proportions and of such concentration (a) that the LDL and VLDL lipoproteins and the chylomicrons are precipitated while (b) the HDL remains in solution, and sedimenting the precipitated lipoproteins, the chylomicrons and the red bloods cells by centrifuging at not more than 1,000 X gravity.
Claim 3
Dependent Claim
29 words
A method as claimed in claim 1 which additionally includes the step of determining the cholesterol, triglyceride, phospholipid or protein content of the supernatant obtained from the centrifuging step.
Claim 4
Dependent Claim
70 words
A method as claimed in claim 2 wherein the reagent additionally contains a colored species which can be used to determine dilution of the supernatant, and which includes the additional steps of determining the dilution of the supernatant and the dilution factor, and multiplying the value determined for cholesterol, triglyceride, phospholipid or protein content of the supernatant times the dilution factor to determine the corresponding content of the blood sample.
Claim 5
Dependent Claim
32 words
A method as claimed in claim 1 wherein substantially one volume of the blood sample is mixed with one volume of the aqueous solution of magnesium ⁺⁺ cations and dextran sulfate 500.
Claim 6
Dependent Claim
51 words
An aqueous solution composed of magnesium ⁺⁺ cations and dextran sulfate 500 in such proportions and of such concentration that, when a whole blood sample of suitable size is added to the solution, (a) the LDL and VLDL lipoproteins and the chylomicrons are precipitated while (b) the HDL remains in solution.
Claim 7
Dependent Claim
51 words
An aqueous solution composed of magnesium ⁺⁺ cations and dextran sulfate 500 in such proportions and of such concentration that, when a whole blood sample of suitable size is added to the solution, (a) the LDL and VLDL lipoproteins and the chylomicrons are precipitated while (b) the HDL remains in solution.
Claim 8
Dependent Claim
28 words
An aqueous solution as claimed in claim 5 which additionally contains a color body which enables the use of absorbance measurements to determine the dilution of the solution.
Claim 9
Dependent Claim
19 words
An aqueous solution as claimed in claim 5 wherein the magnesium ⁺⁺ ions are derived from a magnesium sulfate.
Claim 10
Dependent Claim
55 words
An aqueous solution as claimed in claim 6 wherein the magnesium ⁺⁺ ions are derived from a magnesium sulfate and are present in an amount of substantially mM/l, wherein the dextran sulfate 500 is present in an amount of substantially 1.0 g/l, and wherein the color body is an iron ⁺⁺ complex with 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid.