Patent EP0402272A2 successfully refreshed from Google Patents.
EP0402272A2
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EP
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Cell-culturing substrate, cell-culturing substrate unit bioreactor and extracorporeal circulation type therapeutic apparatus
Assignee: Terumo Corp
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Filing: 1990-06-08
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Granted: —
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Expires: 2010-06-08
Abstract
Abstract A cell-culturing substrate having formed in at least one of the opposite surfaces of a high molecular substrate a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, a cell-culturing substrate unit using thereof, and bioreactor using the unit.
Inventors
- 👤 Makoto C/O Terumo Kabushiki Kaisha Ohnishi
- 👤 Ayako C/O Terumo Kabushiki Kaisha Makino
- 👤 Kenichi C/O Terumo Kabushiki Kaisha Shimura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 21:58
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61M; A61M1/00; A61M1/36; A61M1/3687; A61M1/3689; C12M; C12M21/00; C12M21/08; C12M25/00; C12M25/10; C12M29/00; C12M29/10; C12M29/16; C12N2533/00; C12N2533/30
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
42 words
A cell-culturing substrate having formed in at least one of the opposite surfaces of a high molecular substrate a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof.
Claim 2
Dependent Claim
42 words
A cell-culturing substrate having formed in at least one of the opposite surfaces of a high molecular substrate a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof.
Claim 3
Dependent Claim
15 words
A substrate accoridng to claim 1, wherein said high molecular substrate is a porous membrane.
Claim 4
Dependent Claim
60 words
A substrate according to claim 1, wherein said synthetic high molecular membrane layer has as a component thereof a monomer containing in the molecular unit thereof a nitrogen-containing heterocycle in an amount such that the nitrogen atom/carbon atom ratio in said synthetic high molecular membrane layer determined with an X-ray photoelectric spectrum is in the range of 0.01 to 0.3.
Claim 5
Dependent Claim
59 words
A substrate according to claim 1, wherein said α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof is supplied in the gaseous state to a high molecular substrate possessing a graft-polymerizable polymerization initiating point at least one of the opposite surfaces thereof and is graft polymerized to said membrane type high molecular substrate on said surface.
Claim 6
Dependent Claim
44 words
A substrate according to claim 2, wherein said high molecular substrate has as a main component thereof at least one member selected from the group consisting of polyolefins and polyolefins having part or whole of the hydrogen atoms thereof substituted with a halogen atom.
Claim 7
Dependent Claim
31 words
A substrate according to claim 1, wherein minute projections are formed as spaced with a substantially fixed interval on at least one of the opposite surfaces of said high molecular substrate.
Claim 8
Dependent Claim
91 words
A cell-culturing substrate unit, comprising at least two high molecular substrates of porous membrane combined as opposed to each other, a cell-culturing substrate having formed in at least one of the opposite surfaces of said high molecular substrate a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, a seal formed along the matched peripheral parts of said high molecular substrates, and annular sealing members formed along the outer peripheries of supply mouths perforated in said substrates.
Claim 9
Dependent Claim
91 words
A cell-culturing substrate unit, comprising at least two high molecular substrates of porous membrane combined as opposed to each other, a cell-culturing substrate having formed in at least one of the opposite surfaces of said high molecular substrate a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, a seal formed along the matched peripheral parts of said high molecular substrates, and annular sealing members formed along the outer peripheries of supply mouths perforated in said substrates.
Claim 10
Dependent Claim
60 words
A unit according to claim 7, wherein said synthetic high molecular membrane layer has as a component thereof a monomer containing in the molecular unit thereof a nitrogen-containing heterocycle in an amount such that the nitrogen atom/carbon atom ratio in said synthetic high molecular membrane layer determined with an X-ray photoelectric spectrum is in the range of 0.01 to 0.3.
Claim 11
Dependent Claim
59 words
A unit according to claim 7, wherein said α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof is supplied in the gaseous state to a high molecular substrate possessing a graft-polymerizable polymerization initiating point at least one of the opposite surfaces thereof and is graft polymerized to said membrane type high molecular substrate on said surface.
Claim 12
Dependent Claim
44 words
A unit according to claim 7, wherein said high molecular substrate has as a main component thereof at least one member selected from the group consisting of polyolefins and polyolefins having part or whole of the hydrogen atoms thereof substituted with a halogen atom.
Claim 13
Dependent Claim
31 words
A unit according to claim 7, wherein minute projections are formed as spaced with a substantially fixed interval on at least one of the oppsoite surfaces of said high molecular substrate.
Claim 14
Dependent Claim
113 words
A bioreactor comprising a first compartment and a second compartment for a reaction solution, a microorganism- or cell-culturing substrate having formed in at least one of the opposite surfaces of a high molecular substrate of porous membrane a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, the microorganism- or cell-culturing substrate serving to partition said first and second compartments, and a reaction solution-exchanging mouth formed in said second compartment and adapted to effect exchange of the reaction solution for at least one member selected from the group consisting of microorganisms and cells and stowed in said first compartment.
Claim 15
Dependent Claim
113 words
A bioreactor comprising a first compartment and a second compartment for a reaction solution, a microorganism- or cell-culturing substrate having formed in at least one of the opposite surfaces of a high molecular substrate of porous membrane a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, the microorganism- or cell-culturing substrate serving to partition said first and second compartments, and a reaction solution-exchanging mouth formed in said second compartment and adapted to effect exchange of the reaction solution for at least one member selected from the group consisting of microorganisms and cells and stowed in said first compartment.
Claim 16
Dependent Claim
32 words
A bioreactor according to claim 12, wherein said porous membrane type high molecular substrate is provided with through holes having an average diameter in the range of 5 Å to 10 µm.
Claim 17
Dependent Claim
60 words
A bioreactor according to claim 12, wherein said synthetic high molecular membrane layer has as a component thereof a monomer containing in the molecular unit thereof a nitrogen-containing heterocycle in an amount such that the nitrogen atom/carbon atom ratio in said synthetic high molecular membrane layer determined with an X-ray photoelectric spectrum is in the range of 0.01 to 0.3.
Claim 18
Dependent Claim
51 words
A bioreactor according to claim 12, wherein said porous membrane type high molecular substrate is in the form of a hollow fiber and the outside of said microorganism- or cell-culturing substrate having said synthetic high molecular layer formed on the surface of said hollow fiber substrate constitutes itself said first compartment.
Claim 19
Dependent Claim
122 words
A bioreactor according to claim 12, wherein said porous membrane type high molecular substrate is in the shape of a flat membrane and the interior of the cell-culturing substrate unit comprising at least two high molecular substrates of porous membrane combined as opposed to each other, a cell-culturing substrate having formed in at least one of the opposite surfaces of said high molecular substrate at synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, a seal formed along the matched peripheral parts of said high molecular substrates, and annular sealing members formed along the outer peripheries of supply mouths perforated in said substrates constitutes itself said first compartment.
Claim 20
Dependent Claim
110 words
An extracorporeal circulation type therapeutic apparatus, comprising a first compartment and a second compartment for a reaction solution, a microorganism- or cell-culturing substrate having formed in at least one of the opposite surfaces of a high molecular substrate of porous membrane a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, the microorganism- or cell-culturing substrate serving to partition said first and second compartments, at least one member selected from the group consisting of microorganisms and cells and stowed in said first compartment, and an outlet and an inlet for perfusing blood to said second compartment.
Claim 21
Dependent Claim
110 words
An extracorporeal circulation type therapeutic apparatus, comprising a first compartment and a second compartment for a reaction solution, a microorganism- or cell-culturing substrate having formed in at least one of the opposite surfaces of a high molecular substrate of porous membrane a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, the microorganism- or cell-culturing substrate serving to partition said first and second compartments, at least one member selected from the group consisting of microorganisms and cells and stowed in said first compartment, and an outlet and an inlet for perfusing blood to said second compartment.
Claim 22
Dependent Claim
33 words
A therapeutic apparatus according to claim 17, wherein said porous membrane type high molecular substrate is provided with through holes having an average diameter in the range of 5 Å to 10 µm.
Claim 23
Dependent Claim
61 words
A therapeutic apparatus according to claim 17, wherein said synthetic high molecular membrane layer has as a component thereof a monomer containing in the molecular unit thereof a nitrogen-containing heterocycle in an amount such that the nitrogen atom/carbon atom ratio in said synthetic high molecular membrane layer determined with an X-ray photoelectric spectrum is in the range of 0.01 to 0.3.
Claim 24
Dependent Claim
52 words
A therapeutic apparatus according to claim 17, wherein said porous membrane type high molecular substrate is in the form of a hollow fiber and the outside of said microorganism- or cell-culturing substrate having said synthetic high molecular layer formed on the surface of said hollow fiber substrate constitutes itself said first compartment.
Claim 25
Dependent Claim
123 words
A therapeutic apparatus according to claim 17, wherein said porous membrane type high molecular substrate is in the shape of a flat membrane and the interior of the cell-culturing substraste unit comprising at least two high molecular substrates of porous membrane combined as opposed to each other, a cell-culturing substrate having formed in at least one of the opposite surfaces of said high molecular substrate a synthetic high molecular membrane layer using as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof, a seal formed along the matched peripheral parts of said high molecular substrates, and annular sealing members formed along the outer peripheries of supply mouths perforated in said substrates contitutes itself said first compartment.
Claim 26
Dependent Claim
27 words
A cell-culturing porous membrane provided on at least the cell-culturing side surface thereof with a region inhibiting adhesion of cells and a region allowing adhesion of cells.
Claim 27
Dependent Claim
27 words
A cell-culturing porous membrane provided on at least the cell-culturing side surface thereof with a region inhibiting adhesion of cells and a region allowing adhesion of cells.
Claim 28
Dependent Claim
77 words
A porous membrane according to claim 22, wherein the surface area ratio of region inhibiting adhesion of cells/region allowing adhesion of cells on said cell-culturing surface is in the range of 0.02 to 20, said two regions are separated in the form of a lattice, parallel strips, or islands in sea, the wall thickness of said membrane is in the range of 1 to 500 µm, and the porosity of said membrane is not less than 20%.
Claim 29
Dependent Claim
54 words
A porous membrane according to claim 22, wherein said region inhibiting adhesion of cells is formed of a hydrogen-like surface and said region allowing adhesion of cells is formed of a synthetic high molecular membrane layer having as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof.
Claim 30
Dependent Claim
31 words
A cell-culturing apparatus using a cell-culturing porous membrane provided on at least the cell-culturing side membrane surface with a region inhibiting adhesion of cells and a region allowing adhesion of cells.
Claim 31
Dependent Claim
31 words
A cell-culturing apparatus using a cell-culturing porous membrane provided on at least the cell-culturing side membrane surface with a region inhibiting adhesion of cells and a region allowing adhesion of cells.
Claim 32
Dependent Claim
77 words
A cell-culturing apparatus according to claim 25, wherein the surface area ratio of region inhibiting adhesion of cells/region allowing adhesion of cells on said cell-culturing surface is in the range of 0.02 to 20, said two regions are separated in the form of a lattice, parallel strips, or islands in sea, the wall thickness of said membrane is in the range of 1 to 500 µm, and the porosity of said membrane is not less than 20%.
Claim 33
Dependent Claim
54 words
A cell-culturing apparatus according to claim 25, wherein said region inhibiting adhesion of cells is formed of a hydrogen-like surface and said region allowing adhesion of cells is formed of a synthetic high molecular membrane layer having as a component thereof an α,β-ethylenically unsaturated monomer possessing a nitrogen-containing heterocycle in the molecular unit thereof.
References Cited (Backward Citations)
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