Patent EP0392225A2 successfully refreshed from Google Patents.
EP0392225A2
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EP
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Disease-resistant transgenic plants
Assignee: Syngenta Participations AG
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Filing: 1990-03-21
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Granted: —
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Expires: 2010-03-21
Abstract
Abstract The present invention relates to chimeric DNA constructs useful for producing transgenic disease-resistant plants and to genetic engineering of plants to produce the phenotype of disease resistance. In particular it relates to constitutive expression in transgenic plants of DNA sequences which encode pathogenesis-related proteins (PRPs) A further objective of the present invention are transgenic plants constitutively expressing induced levels of plant PRPs or substantially homologous proteins, providing an enhanced disease-resistant phenotype with respect to wild-type plants. Another object of the present invention is to provide transgenic plants constitutively transcribing sense or antisense mRNA strands of DNA sequences encoding plant PRPs or transcribing sense or antisense mRNA strands of DNA sequences substantially homologous to genomic or cDNA sequences encoding plant PRPs, such transgenic plants thus having an enhanced disease-resistant phenotype with respect to wild-type plants.
Inventors
- 👤 John A. Dr. Ryals
- 👤 Danny C. Alexander
- 👤 Robert M. Goodman
- 👤 Frederick Prof. Meins
- 👤 George B. Payne
- 👤 Jeffrey R. Stinson
- 👤 Jean-Marc Dr. Neuhaus
- 👤 Mary B Moyer
- 👤 Eric Russell Ward
- 👤 Shericca Cherrer Williams
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 06:07
CPC Classifications
C; C12; C12Y; C12Y302/00; C12Y302/01; C12Y302/01039; C12N; C12N15/00; C12N15/09; C12N15/11; C07; C07K; C07K14/00; C07K14/415; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8237; C12N15/8239; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8282; C12N9/00; C12N9/0004; C12N9/0065; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/244; C12N9/2442; C12Y302/01006; C12Y302/01014; C12Y302/01058; C12Y302/01073; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/55; C07K2319/61
Claims Analysis
Total Claims: 86
Claim 1
Independent Claim
77 words
A chimeric DNA sequence which comprises: (a) a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence, and (b) a second DNA sequence comprising a coding sequence of an inducible plant pathogenesis-related protein, or a coding sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein, said second DNA sequence being in either a sense or antisense orientation with respect to said first DNA sequence.
Claim 2
Dependent Claim
77 words
A chimeric DNA sequence which comprises: (a) a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence, and (b) a second DNA sequence comprising a coding sequence of an inducible plant pathogenesis-related protein, or a coding sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein, said second DNA sequence being in either a sense or antisense orientation with respect to said first DNA sequence.
Claim 3
Dependent Claim
23 words
A chimeric DNA sequence of claim 1, wherein the first DNA sequence is of heterologous origin with respect to the second DNA sequence.
Claim 4
Dependent Claim
26 words
A chimeric DNA sequence according to claim 2, wherein the first DNA sequence is the promoter of the small subunit of tobacco ribulose bis-phosphate carboxylase (RUBISCO).
Claim 5
Dependent Claim
18 words
A chimeric DNA sequence according to claim 2, wherein the first DNA sequence comprises double CaMV 35S promoter.
Claim 6
Dependent Claim
50 words
A chimeric DNA sequence of claim 1, wherein the second DNA sequence encodes a plant pathogenesis-related protein selected from the group consisting of PR-1A, PR-1B, PR-1C, PR-R major, PR-R minor, PR-P, PR-Q, PR-2, PR-N, PR-O, PR-O′, SAR8.2a, SAR8.2b, cucumber chitinase/lysozyme, cucumber basic peroxidase, tobacco basic glucanase and tobacco basic chitinase.
Claim 7
Dependent Claim
44 words
A chimeric DNA sequence of claim 1, wherein the second DNA sequence encodes a plant pathogenesis-related protein selected from the group consisting of PR-1A, PR-1B, PR-1C, PR-R major, PR-R minor, PR-P, PR-Q, PR-O′, SAR8.2a, SAR8.2b, cucumber chitinase/lysozyme, tobacco basic glucanase and tobacco basic chitinase.
Claim 8
Dependent Claim
47 words
A chimeric DNA sequence of claim 1, wherein the second DNA sequence encodes a plant pathogenesis-related protein selected from the group consisting of PR-R major, PR-R minor, PR-P, PR-Q, PR-2, PR-N, PR-O, PRO′, SAR8.2a, SAR8.2b, cucumber chitinase/lysozyme, cucumber basic peroxidase, tobacco basic glucanase and tobacco basic chitinase.
Claim 9
Dependent Claim
38 words
A chimeric DNA sequence of claim 1, wherein the second DNA sequence is oriented with respect to the first DNA sequence such that the first DNA sequence promotes transcription of the sense strand of the second DNA sequence.
Claim 10
Dependent Claim
38 words
A chimeric DNA sequence of claim 1, wherein the second DNA sequence is oriented with respect to the first DNA sequence such that the first DNA sequence promotes transcription of the antisense strand of the second DNA sequence.
Claim 11
Dependent Claim
54 words
A chimeric DNA sequence which comprises: (a) a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence, and (b) a second DNA sequence encoding an inducible plant pathogenesis-related protein, or encoding a sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein.
Claim 12
Dependent Claim
54 words
A chimeric DNA sequence which comprises: (a) a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence, and (b) a second DNA sequence encoding an inducible plant pathogenesis-related protein, or encoding a sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein.
Claim 13
Dependent Claim
23 words
A chimeric DNA sequence of claim 10, wherein the first DNA sequence is of heterologous origin with respect to the second DNA sequence.
Claim 14
Dependent Claim
16 words
A recombinant DNA molecule comprising a chimeric DNA sequence of anyone of claims 1 to 11.
Claim 15
Dependent Claim
18 words
A recombinant DNA molecule according to claim 12, which is characterized in that it is a vector molecule.
Claim 16
Dependent Claim
10 words
A recombinant DNA molecule according to claim 12 comprising pCGN1703.
Claim 17
Dependent Claim
20 words
Plants, plant tissue, plant propagules or plant seed containing a chimeric DNA sequence of anyone of claims 1 to 11.
Claim 18
Dependent Claim
75 words
Plant, plant tissue, plant propagules or plant seed containing a chimeric DNA sequence comprising a first DNA sequence comprising the promoter of the small subunit of tobacco ribulose bis-phosphate carboxylase (RUBISCO) and a second DNA sequence comprising the coding sequence of PR-1A pathogenesis-related protein, wherein the second DNA sequence is oriented with respect to the first DNA sequence such that the first DNA sequence promotes transcription of the antisense strand of the second DNA sequence.
Claim 19
Dependent Claim
75 words
Plant, plant tissue, plant propagules or plant seed containing a chimeric DNA sequence comprising a first DNA sequence comprising the promoter of the small subunit of tobacco ribulose bis-phosphate carboxylase (RUBISCO) and a second DNA sequence comprising the coding sequence of PR-1A pathogenesis-related protein, wherein the second DNA sequence is oriented with respect to the first DNA sequence such that the first DNA sequence promotes transcription of the antisense strand of the second DNA sequence.
Claim 20
Dependent Claim
16 words
Plants, plant tissue, plant propagules or plant seed of claim 15, wherein the plant is tobacco.
Claim 21
Dependent Claim
23 words
A transgenic plant constitutively expressing induced levels of plant pathogenesis-related proteins or constitutively expressing levels of pathogenesis-related proteins which provide a disease-resistant phenotype.
Claim 22
Dependent Claim
23 words
A transgenic plant constitutively expressing induced levels of plant pathogenesis-related proteins or constitutively expressing levels of pathogenesis-related proteins which provide a disease-resistant phenotype.
Claim 23
Dependent Claim
22 words
A transgenic plant constitutively transcribing sense or antisense strands of DNA sequences encoding a plant pathogenesis-related protein which provides a disease-resistant phenotype.
Claim 24
Dependent Claim
22 words
A transgenic plant constitutively transcribing sense or antisense strands of DNA sequences encoding a plant pathogenesis-related protein which provides a disease-resistant phenotype.
Claim 25
Dependent Claim
20 words
A substantially pure cDNA sequence encoding SAR8.2a, or a DNA sequence having substantial sequence homology to cDNA encoding SAR 8.2a.
Claim 26
Dependent Claim
20 words
A substantially pure cDNA sequence encoding SAR8.2a, or a DNA sequence having substantial sequence homology to cDNA encoding SAR 8.2a.
Claim 27
Dependent Claim
10 words
Plasmid pCIB/SAR8.2a, ATCC accession number 40584, deposited March 22, 1989.
Claim 28
Dependent Claim
10 words
Plasmid pCIB/SAR8.2a, ATCC accession number 40584, deposited March 22, 1989.
Claim 29
Dependent Claim
20 words
A substantially pure cDNA sequence encoding SAR8.2b, or a DNA sequence having substantial sequence homology to cDNA encoding SAR 8.2b.
Claim 30
Dependent Claim
20 words
A substantially pure cDNA sequence encoding SAR8.2b, or a DNA sequence having substantial sequence homology to cDNA encoding SAR 8.2b.
Claim 31
Dependent Claim
10 words
Plasmid pCIB/SAR8.2b, ATCC accession number 40585, deposited March 22, 1989.
Claim 32
Dependent Claim
10 words
Plasmid pCIB/SAR8.2b, ATCC accession number 40585, deposited March 22, 1989.
Claim 33
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-P, or a DNA sequence having substantial sequence homology to cDNA encoding PR-P.
Claim 34
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-P, or a DNA sequence having substantial sequence homology to cDNA encoding PR-P.
Claim 35
Dependent Claim
10 words
Plasmid pBScht28, ATCC accession number 40588, deposited March 24, 1989.
Claim 36
Dependent Claim
10 words
Plasmid pBScht28, ATCC accession number 40588, deposited March 24, 1989.
Claim 37
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-2, or a DNA sequence having substantial sequence homology to cDNA encoding PR-2.
Claim 38
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-2, or a DNA sequence having substantial sequence homology to cDNA encoding PR-2.
Claim 39
Dependent Claim
10 words
Plasmid pBSGL117, ATCC accession number 40691, deposited Octpber 19, 1989.
Claim 40
Dependent Claim
10 words
Plasmid pBSGL117, ATCC accession number 40691, deposited Octpber 19, 1989.
Claim 41
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-N, or a DNA sequence having substantial sequence homology to cDNA encoding PR-N.
Claim 42
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-N, or a DNA sequence having substantial sequence homology to cDNA encoding PR-N.
Claim 43
Dependent Claim
10 words
Plasmid pBSGL148, ATCC accession number 40689, deposited October 19, 1989.
Claim 44
Dependent Claim
10 words
Plasmid pBSGL148, ATCC accession number 40689, deposited October 19, 1989.
Claim 45
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-O, or a DNA sequence having substantial sequence homology to cDNA encoding PR-O.
Claim 46
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-O, or a DNA sequence having substantial sequence homology to cDNA encoding PR-O.
Claim 47
Dependent Claim
10 words
Plasmid pBSGL134, ATCC accession number 40690, deposited October 19, 1989.
Claim 48
Dependent Claim
10 words
Plasmid pBSGL134, ATCC accession number 40690, deposited October 19, 1989.
Claim 49
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-2', or a DNA sequence having substantial sequence homology to cDNA encoding PR-2'.
Claim 50
Dependent Claim
19 words
A substantially pure cDNA sequence encoding PR-2', or a DNA sequence having substantial sequence homology to cDNA encoding PR-2'.
Claim 51
Dependent Claim
10 words
Plasmid pBSGL135, ATCC accession number 40685, deposited October 19, 1989.
Claim 52
Dependent Claim
10 words
Plasmid pBSGL135, ATCC accession number 40685, deposited October 19, 1989.
Claim 53
Dependent Claim
21 words
A substantially pure cDNA sequence encoding cucumber peroxidase, or a DNA sequence having substantial sequence homology to cDNA encoding cucumber peroxidase.
Claim 54
Dependent Claim
21 words
A substantially pure cDNA sequence encoding cucumber peroxidase, or a DNA sequence having substantial sequence homology to cDNA encoding cucumber peroxidase.
Claim 55
Dependent Claim
10 words
Plasmid pBPER1, ATCC accession number 40686, deposited October 19, 1989.
Claim 56
Dependent Claim
10 words
Plasmid pBPER1, ATCC accession number 40686, deposited October 19, 1989.
Claim 57
Dependent Claim
10 words
Plasmid pBPERB24, ATCC accession number 40687, deposited October 19, 1989.
Claim 58
Dependent Claim
10 words
Plasmid pBPERB24, ATCC accession number 40687, deposited October 19, 1989.
Claim 59
Dependent Claim
20 words
A substantially pure DNA sequence of "Sequence 6" or having substantial sequence homology to the sequence shown in "Sequence 6."
Claim 60
Dependent Claim
20 words
A substantially pure DNA sequence of "Sequence 6" or having substantial sequence homology to the sequence shown in "Sequence 6."
Claim 61
Dependent Claim
20 words
A substantially pure DNA sequence of "Sequence 9" or having substantial sequence homology to the sequence shown in "Sequence 9."
Claim 62
Dependent Claim
20 words
A substantially pure DNA sequence of "Sequence 9" or having substantial sequence homology to the sequence shown in "Sequence 9."
Claim 63
Dependent Claim
20 words
A substantially pure DNA sequence of "Sequence 10" or having substantial sequence homology to the sequence shown in "Sequence 10."
Claim 64
Dependent Claim
20 words
A substantially pure DNA sequence of "Sequence 10" or having substantial sequence homology to the sequence shown in "Sequence 10."
Claim 65
Dependent Claim
144 words
A method for producing a chimeric DNA sequence of claim 1, which method comprises: (a) preparing from a suitable source a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence; (b) preparing a DNA library from pathogen infected or from an infevted and an uninfected plant tissue, the said library thus comprising induced or both induced and uninduced DNA populations and isolating therefrom a second DNA sequence comprising a coding sequence of an inducible plant pathogenesis-related protein, or a coding sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein; and (c) operably linking the first DNA seqeuence of step (a) with the second DNA sequence of step (b) such that the said second DNA sequence is in either a sense or an antisense orientation with respect to said first DNA sequence.
Claim 66
Dependent Claim
22 words
A method according to claim 40, wherein the first DNA sequence is of heterologous origin with respect to the second DNA sequence.
Claim 67
Dependent Claim
26 words
A method according to claim 41, wherein the first DNA sequence is prepared from a gene encoding the small subunit of tobacco ribulose bis-phosphate carboxylase (RUBISCO).
Claim 68
Dependent Claim
22 words
A method according to claim 41, wherein the first DNA sequence, being prepared from the CaMV genome, comprises double CaMV 35S promoter.
Claim 69
Dependent Claim
23 words
A method according to claim 40, wherein the plant tissue is induced to systemic acquired resistance or localized acquired resistance using biological inducers.
Claim 70
Dependent Claim
49 words
A method according to claim 40, wherein the second DNA sequence encodes a plant pathogenesis-related protein selected from the group consisting of PR-1A, PR-1B, PR-1C, PR-R major, PR-R minor, PR-P, PR-Q, PR-2, PR-N, PR-O, PR-O′, SAR8.2a, SAR8.2b, cucumber chitinase/lysozyme, cucumber basic peroxidase, tobacco basic glucanase and tobacco basic chitinase.
Claim 71
Dependent Claim
53 words
A method according to claim 40, wherein linking the first and the second DNA sequence has been carried out in a way that the second DNA sequence is oriented with respect to the first DNA sequence such that the first DNA sequence promotes transcription of the sense strand of the second DNA sequence.
Claim 72
Dependent Claim
53 words
A method according to claim 40, wherein linking the first and the second DNA sequence has been carried out in a way that the second DNA sequence is oriented with respect to the first DNA sequence such that the first DNA sequence promotes transcription of the antisense strand of the second DNA sequence.
Claim 73
Dependent Claim
133 words
A method for producing a chimeric DNA sequence of claim 10, which method comprises: (a) preparing from a suitable source a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence; (b) preparing a DNA library from pathogen infected or from an infected and an uninfected plant tissue, the said library thus comprising induced or both induced and uninduced DNA populations and isolating therefrom a DNA sequence encoding an inducible plant pathogenesis-related protein, or encoding a sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein; and (c) linking the first DNA seqeuence of step (a) with the second DNA sequence of step (b) in an operable manner, such that the first DNA sequence promotes the transcription of the second DNA sequence.
Claim 74
Independent Claim
105 words
A method for producing a transgenic plant cell or plant tissue having the potential to regenerate into a plant exhibiting a disease-resistant phenotype, comprising transforming the plant cell or tissue with a chimeric DNA sequence comprising a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence, and a second DNA sequence comprising a coding sequence of an inducible plant pathogenesis-related protein, or a coding sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein, said second DNA sequence being in either a sense or antisense orientation with respect to said first DNA sequence.
Claim 75
Independent Claim
105 words
A method for producing a transgenic plant cell or plant tissue having the potential to regenerate into a plant exhibiting a disease-resistant phenotype, comprising transforming the plant cell or tissue with a chimeric DNA sequence comprising a first DNA sequence which promotes in a plant the constitutive transcription of a second DNA sequence, and a second DNA sequence comprising a coding sequence of an inducible plant pathogenesis-related protein, or a coding sequence having substantial sequence homology to a coding sequence of an inducible plant pathogenesis-related protein, said second DNA sequence being in either a sense or antisense orientation with respect to said first DNA sequence.
Claim 76
Dependent Claim
32 words
A method for producing a transgenic plant cell or plant tissue according to claim 49, wherein the plant cell or plant tissue being part of a plant embryo or a whole plant.
Claim 77
Dependent Claim
27 words
A method for producing a transgenic plant exhibiting a disease-resistant phenotype comprising regenerating a whole plant from the transformed plant tissue or plant cell of claim 49.
Claim 78
Independent Claim
28 words
A method according to anyone of claims 49 to 51, wherein the transformation is carried out using a chimeric DNA sequence of anyone of claims 1 to 11.
Claim 79
Dependent Claim
29 words
A method for producing a plant having a disease-resistant phenotype, comprising transforming plant tissue or cells with a chimeric DNA sequence comprising a first DNA sequence of claim 1.
Claim 80
Dependent Claim
22 words
A method of claim 53, wherein the chimeric DNA sequence further comprises a second DNA sequence which is a plant-selectable gene marker.
Claim 81
Dependent Claim
10 words
A disease-resistant plant produced by a method of claim 53.
Claim 82
Dependent Claim
14 words
A disease-resistant plant produced by a method of anyone of claims 49 to 52.
Claim 83
Independent Claim
89 words
A method for differential screening and enrichment of cDNA populations, comprising: (a) providing single-stranded cDNA from induced and uninduced populations, the single-stranded cDNA from the induced and uninduced populations having opposite DNA polarity, and the cDNA from the uninduced population having a biotin-affinity tag; (b) hybridizing the single-stranded cDNA populations of step (a) with each other, and (c) separating the hybridization mixture of step (b) by biotin-avidin chromatography to enrich for single stranded cDNAs from the induced population which are not hybridized to the cDNA from the uninduced population.
Claim 84
Independent Claim
89 words
A method for differential screening and enrichment of cDNA populations, comprising: (a) providing single-stranded cDNA from induced and uninduced populations, the single-stranded cDNA from the induced and uninduced populations having opposite DNA polarity, and the cDNA from the uninduced population having a biotin-affinity tag; (b) hybridizing the single-stranded cDNA populations of step (a) with each other, and (c) separating the hybridization mixture of step (b) by biotin-avidin chromatography to enrich for single stranded cDNAs from the induced population which are not hybridized to the cDNA from the uninduced population.
Claim 85
Independent Claim
32 words
A method for cloning cDNAs encoding disease-resistance proteins comprising (a) providing tissue induced to systemic acquired resistance or localized acquired resistance using biological inducers, and (b) isolating cDNA clones encoding disease-resistance proteins.
Claim 86
Independent Claim
32 words
A method for cloning cDNAs encoding disease-resistance proteins comprising (a) providing tissue induced to systemic acquired resistance or localized acquired resistance using biological inducers, and (b) isolating cDNA clones encoding disease-resistance proteins.
References Cited (Backward Citations)
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