Patent EP0389063A2 successfully refreshed from Google Patents.
EP0389063A2
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EP
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Process for isolating nucleic acid
Assignee: Biomerieux BV
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Filing: 1990-03-22
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Granted: —
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Expires: 2010-03-22
Abstract
Abstract The invention relates to a process, a combination of means for isolating nucleic acid from a nucleic acid-containing starting material and a testkit in order to amplify the nucleic acid obtained by said process. More in particular, the invention relates to a process and a kit for isolating nucleic acid from a nucleic acid-containing biological material such as whole blood, blood serum, urine, feces, cell cultures and the like.
Inventors
- 👤 Willem René Boom
- 👤 Henriette Maria Aleida Adriaanse
- 👤 Tim Kievits
- 👤 Peter Franklin Lens
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 15:15
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
61 words
A process for isolating nucleic acid from a nucleic acid-containing starting material, characterized by mixing the starting material, a chaotropic substance and a nucleic acid binding solid phase, separating the solid phase with the nucleic acid bound thereto from the liquid, whereafter thus obtained solid phase-nucleic acid complexes were washed and if required the nucleic acid was eluted from said complexes.
Claim 2
Dependent Claim
61 words
A process for isolating nucleic acid from a nucleic acid-containing starting material, characterized by mixing the starting material, a chaotropic substance and a nucleic acid binding solid phase, separating the solid phase with the nucleic acid bound thereto from the liquid, whereafter thus obtained solid phase-nucleic acid complexes were washed and if required the nucleic acid was eluted from said complexes.
Claim 3
Dependent Claim
37 words
A process according to claim 1, characterized in that the starting material employed is a nucleic acid-containing biological material, such as whole blood, blood serum, buffy coat, urine, feces, liquor cerebrospinales, sperm, saliva, tissues and cell cultures.
Claim 4
Dependent Claim
34 words
A process according to claim 1 or 2, characterized in that the chaotropic substance employed is selected from the group comprising guanidinium salt, sodium iodide, potassium iodide, sodium (iso)thiocyanate, urea or mutual combinations therewith.
Claim 5
Dependent Claim
16 words
A process according to claim 3 characterized in that the guanidinium salt employed is guanidinium (iso)thiocyanate.
Claim 6
Dependent Claim
33 words
A process according to claim 1 characterized in that the nucleic acid binding solid phase employed is selected from the group comprising silica particles, polymeric material, filter material, polystyrene beads or nitrocellulose paper.
Claim 7
Dependent Claim
14 words
A process according to any of claims 1-5, characterized by isolating DNA and/or RNA.
Claim 8
Dependent Claim
24 words
A process according to any of claims 1-6, characterized by employing silica particles having a particle size ranging substantially between 0.05 and 500 µm.
Claim 9
Dependent Claim
24 words
A process according to any of claims 1-6, characterized by employing silica particles having a particle size ranging substantially between 0.1 and 200 µm.
Claim 10
Dependent Claim
24 words
A process according to any of claims 1-8, characterized by employing silica particles having a particle size ranging substantially between 1 and 200 µm.
Claim 11
Dependent Claim
38 words
A process according to any of claims 1-9, characterized by separating the resulting solid phase-nucleic acid complexes from the liquid by sedimentation and disposal of the supernatant, and then washing the complexes with a chaotropic substance-containing washing buffer.
Claim 12
Dependent Claim
31 words
A process according to claim 10, characterized in that the solid phase-nucleic acid complexes washed with washing buffer are further washed successively with one or more organic solvens, followed by drying.
Claim 13
Dependent Claim
30 words
A process according to claim 11, characterized in that the nucleic acid present in the washed and dried solid phase-nucleic acid complexes is eluted by means of an elution buffer.
Claim 14
Dependent Claim
43 words
A process according to claim 1 characterized in that said thus obtained solid phase-nucleic acid complexes were brought into contact with a mixture wherein components are present in order to amplify the nucleic acid either bound to said solid phase or eluted therefrom.
Claim 15
Dependent Claim
13 words
A combination of means for carrying out the process according to claim 1.
Claim 16
Dependent Claim
12 words
A test kit for carrying out the process according to claim 13.
References Cited (Backward Citations)
No citation records indexed.
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