Patent EP0375406A2 successfully refreshed from Google Patents.
EP0375406A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Transgenic organisms and cells and methods of producing transgenic organisms and cells
Assignee: University of Pennsylvania
•
Filing: 1989-12-20
•
Granted: —
•
Expires: 2009-12-20
Abstract
Abstract Transgenic or transomic organisms and cells and methods of producing transgenic or transomic organisms and cells are disclosed. A chromosome or chromosome fragment of a donor organism is isolated and a substantial portion of the retrieved chromosome or chromosome fragment is inserted into the heritable genetic material of a recipient organism. Methods for the retrieval, manipulation, injection and subsequent insertion of large chromosome fragments into the heritable genetic material of recipient cells are disclosed.
Inventors
- 👤 Cecilia Lo
- 👤 Jean Richa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 05:43
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/10; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; C12N15/00; C12N15/09; C12N15/87; C12N15/90; C12N5/12; C12N5/16; A01K2217/00; A01K2217/05
Claims Analysis
Total Claims: 59
Claim 1
Independent Claim
49 words
A transgenic non-human organism, wherein said organism is an animal or a microbe, comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids.
Claim 2
Dependent Claim
49 words
A transgenic non-human organism, wherein said organism is an animal or a microbe, comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids.
Claim 3
Dependent Claim
23 words
An organism according to claim 1, wherein said exogenous chromosome or said exogenous chromosome fragment contains at least 250 kilobases of nucleic acids.
Claim 4
Dependent Claim
23 words
An organism according to claim 2, wherein said exogenous chromosome or said exogenous chromosome fragment contains at least 1 megabases of nucleic acids.
Claim 5
Dependent Claim
24 words
An organism according to claim 3, wherein said exogenous chromosome or said exogenous chromosome fragment contains from 10 to 30 megabases of nucleic acids.
Claim 6
Dependent Claim
16 words
An organism according to any one of the preceding claims, wherein said organism is a mammal.
Claim 7
Dependent Claim
26 words
An organism according to any one of the preceding claims, wherein said exogenous chromosome and said exogenous chromosome fragment is of animal, plant or microbial origin.
Claim 8
Dependent Claim
40 words
An organism according to any one of the preceding claims, wherein said exogenous chromosome or said exogenous chromosome fragment comprises genetic material which imparts to said organism characteristics useful for modeling pathological conditions arising from infectious agents or environmental agents.
Claim 9
Dependent Claim
18 words
An organism according to claim 7, wherein said infectious agent is an AIDS virus or a hepatitis virus.
Claim 10
Dependent Claim
39 words
A transgenic animal cell comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment contains at least 100 kilobases of nucleic acids.
Claim 11
Dependent Claim
39 words
A transgenic animal cell comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment contains at least 100 kilobases of nucleic acids.
Claim 12
Dependent Claim
15 words
A cell according to claim 9, wherein said cell is a pluripotential embryonic stem cell.
Claim 13
Dependent Claim
40 words
A transgenic animal cell obtained from a transgenic multicellular animal, said multicellular animal comprising a plurality of cells containing at least one exogenous chromosome or at least one exogenous chromosome fragment, each containing at least 100 kilobases of nucleic acids.
Claim 14
Dependent Claim
40 words
A transgenic animal cell obtained from a transgenic multicellular animal, said multicellular animal comprising a plurality of cells containing at least one exogenous chromosome or at least one exogenous chromosome fragment, each containing at least 100 kilobases of nucleic acids.
Claim 15
Dependent Claim
60 words
A cell according to claim 11, wherein said exogenous chromosome or said exogenous chromosome fragment comprises genetic material encoding (i) a human immunoglobulin complex, or a portion thereof, (ii) the human histocompatibility complex, or a portion thereof, (iii) a hormone, a growth factor, a clotting factor or an enzyme, (iv) a heritable disease in humans or (v) an infectious disease.
Claim 16
Dependent Claim
52 words
A transgenic organism, wherein said organism is a plant, comprising at least one selected exogenous chromosome or at least one selectd exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of plant origin.
Claim 17
Dependent Claim
52 words
A transgenic organism, wherein said organism is a plant, comprising at least one selected exogenous chromosome or at least one selectd exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of plant origin.
Claim 18
Dependent Claim
52 words
A transgenic organism, wherein said organism is a plant, comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of animal or microbial origin.
Claim 19
Dependent Claim
52 words
A transgenic organism, wherein said organism is a plant, comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of animal or microbial origin.
Claim 20
Dependent Claim
47 words
A transgenic plant cell, comprising at least one selected exogenous chromosome or at least one selected exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of plant origin.
Claim 21
Dependent Claim
47 words
A transgenic plant cell, comprising at least one selected exogenous chromosome or at least one selected exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of plant origin.
Claim 22
Dependent Claim
47 words
A transgenic plant cell, comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of animal or microbial origin.
Claim 23
Dependent Claim
47 words
A transgenic plant cell, comprising at least one exogenous chromosome or at least one exogenous chromosome fragment in its heritable genetic material, wherein said exogenous chromosome and said exogenous chromosome fragment each contains at least 100 kilobases of nucleic acids and is of animal or microbial origin.
Claim 24
Independent Claim
64 words
A method for transferring at least one chromosome or chromosome fragment from a donor organism to a recipient organism, comprising: (a) isolating a selected chromosome or a selected chromosome fragment of a donor organism; and (b) inserting at least a portion of said selected chromosome or of said selected chromosome fragment of said donor organism into the heritable genetic material of a recipient organism.
Claim 25
Independent Claim
64 words
A method for transferring at least one chromosome or chromosome fragment from a donor organism to a recipient organism, comprising: (a) isolating a selected chromosome or a selected chromosome fragment of a donor organism; and (b) inserting at least a portion of said selected chromosome or of said selected chromosome fragment of said donor organism into the heritable genetic material of a recipient organism.
Claim 26
Dependent Claim
35 words
A method according to claim 17, wherein step (b) comprises inserting said chromosome or said chromosome fragment into a cell of said recipient organism for incorporation into the heritable genetic material of said recipient organism.
Claim 27
Dependent Claim
28 words
A method according to claim 18, wherein said chromosome or said chromosome fragment is inserted into the cytoplasm, pronucleus or nucleus of a cell of said recipient organism.
Claim 28
Independent Claim
19 words
A method according to any one of claims 17 to 19, wherein said recipient organism is a non-human mammal.
Claim 29
Independent Claim
22 words
A method according to any one of claims 17 to 19, wherein said recipient organism is an animal cell or plant cell.
Claim 30
Dependent Claim
17 words
A method according to claim 21, wherein said animal cell is a rodent cell or an oocyte.
Claim 31
Independent Claim
93 words
A method according to any one of claims 17 to 22, wherein step (a) comprises: (a′) preparing the chromosome or chromosomes of said donor organism for retrieval of said chromosome or of said chromosome fragment; (b′) selecting a chromosome or chromosome fragment to be retrieved; (c′) retrieving said chromosome or said chromosome fragment; and (d′) manipulating said retrieved chromosome or said chromosome fragment into a pipette, a microelectrode or a syringe, or encapsulating said chromosome or said chromosome fragment into a vesicle, or loading said chromosomme or said chromosome fragment onto a microprojectile.
Claim 32
Dependent Claim
30 words
A method according to claim 23, wherein step (a′) comprises spreading said chromosome or said chromosomes in a fixative solution onto a surface and said fixative solution is subsequently removed.
Claim 33
Independent Claim
48 words
A method according to any one of claims 17 to 24 wherein said chromosome or chromosome fragment which is inserted into the heritable genetic material of said recipient organism comprises genetic material encoding a human immunoglobulin commplex or portion thereof or a human histocompatability complex or portion thereof.
Claim 34
Independent Claim
45 words
A method according to any one of claims 17 to 24, wherein said chromosome(s) or chromosome fragment(s) which is (are) inserted into the heritable genetic material of said recipient organism comprise(s) genetic material encoding a hormone, a growth factor, a clotting factor or an enzyme.
Claim 35
Independent Claim
36 words
A method accoridng to any one of claims 17 to 24, wherein said chromosome(s) or chromosome fragment(s) which is inserted into the heritable genetic material of said recipient organism is a human chromosome or chromosome frragment.
Claim 36
Independent Claim
39 words
A method according to any one of claims 17 to 24, wherein said chromosome(s) or chromosome fragment(s) which is inserted into the heritable genetic material of said recipient organism comprises human genetic material associated with a disease in humans.
Claim 37
Dependent Claim
30 words
A method according to claim 28, wherein said disease in humans is selected from Huntington's disease, Alzheimer's disease, adult polycystic kidney disease, Down's syndrome, polysyndactyly autoimmune, cardiovascular and metabolic diseases.
Claim 38
Independent Claim
46 words
A method according to any one of claims 17 to 24, wherien said chromosome(s) or chromosome fragment(s) which is inserted into the heritable genetic material of said recipient organism comprises human genetic material associated with an enhanced risk of developing cutaneous malignant melanoma or other cancers.
Claim 39
Dependent Claim
118 words
A transgenic cell containing at least one exogenous chromosome or exogenous chromosome fragment introduced into said transgenic cell, or an antecedent generation of said transgenic cell, by a method for transferring a chromosome or chromosome fragment from a donor organism to a recipient cell, which method comprises: (a) isolating a chromosome or chromosome fragment of a donor organism; and (b) inserting a portion, comprising more than 100 kilobases of nucleic acids, of said chromosome or of said chromosome fragment of said donor organism into the heritable genetic material of a recipient cell (b1) thereby obtaining said transgenic cell or (b2) obtaining a transgenic animal from said recipient cell and subsequently obtaining said transgenic cell from said transgenic animal.
Claim 40
Dependent Claim
118 words
A transgenic cell containing at least one exogenous chromosome or exogenous chromosome fragment introduced into said transgenic cell, or an antecedent generation of said transgenic cell, by a method for transferring a chromosome or chromosome fragment from a donor organism to a recipient cell, which method comprises: (a) isolating a chromosome or chromosome fragment of a donor organism; and (b) inserting a portion, comprising more than 100 kilobases of nucleic acids, of said chromosome or of said chromosome fragment of said donor organism into the heritable genetic material of a recipient cell (b1) thereby obtaining said transgenic cell or (b2) obtaining a transgenic animal from said recipient cell and subsequently obtaining said transgenic cell from said transgenic animal.
Claim 41
Dependent Claim
109 words
A non-human transgenic animal whose germ cells and somatic cells contain at least one exogenous chromosome or exogenous chromosome fragment introduced into an embryonic stage of said animal, or an ancestor of said animal, by a method for transferring a chromosome or chromosome fragment from a donor organism to a recipient organism, which method comprises: (a) isolating a chromosome or chromosome fragment of a donor organism; (b) inserting a portion, comprising more than 100 kilobases of nucleic acids, of said chromosome or said chromosome fragment of said donor organism into the heritable genetic material of a recipient cell; and (c) obtaining said non-human transgenic animal from said recipient cell.
Claim 42
Dependent Claim
109 words
A non-human transgenic animal whose germ cells and somatic cells contain at least one exogenous chromosome or exogenous chromosome fragment introduced into an embryonic stage of said animal, or an ancestor of said animal, by a method for transferring a chromosome or chromosome fragment from a donor organism to a recipient organism, which method comprises: (a) isolating a chromosome or chromosome fragment of a donor organism; (b) inserting a portion, comprising more than 100 kilobases of nucleic acids, of said chromosome or said chromosome fragment of said donor organism into the heritable genetic material of a recipient cell; and (c) obtaining said non-human transgenic animal from said recipient cell.
Claim 43
Dependent Claim
11 words
An animal according to claim 32, which is a non-human mammal.
Claim 44
Dependent Claim
13 words
An animal according to claim 33, wherein said non-human mammal is a mouse.
Claim 45
Independent Claim
93 words
A method for obtaining a transgenic animal having a plurality of cells containing at least one exogenous chromosome or exogenous chromosome fragment, said method comprising: (a˝˝) introducing at least one selected exogenous chromosome or at least one selected exogenous chromosome fragment into the heritable genetic material of an embryonic animal recipient cell; (b˝˝) transplanting said embryonic animal recipient cell containing said exogenous chromosome or said exogenous chromosome fragment into the oviduct or uterus of a female; and (c˝˝) allowing said embryonic animal recipient cell to develop to term to obtain said transgenic animal.
Claim 46
Independent Claim
93 words
A method for obtaining a transgenic animal having a plurality of cells containing at least one exogenous chromosome or exogenous chromosome fragment, said method comprising: (a˝˝) introducing at least one selected exogenous chromosome or at least one selected exogenous chromosome fragment into the heritable genetic material of an embryonic animal recipient cell; (b˝˝) transplanting said embryonic animal recipient cell containing said exogenous chromosome or said exogenous chromosome fragment into the oviduct or uterus of a female; and (c˝˝) allowing said embryonic animal recipient cell to develop to term to obtain said transgenic animal.
Claim 47
Independent Claim
97 words
A method for obtaining a transgenic animal having a plurality of cells containing at least one exogenous chromosome or exogenous chromosome fragment, said method comprising: (a) introducing at least one exogenous chromosome or exogenous chromosome fragment, each containing at least 100 kilobases of nucleic acids, into the heritable genetic material of an embryonic animal receipient cell; (b) generating a pluripotential embryonic stem cell line from said embryonic animal recipient cell; (c) transplanting said pluripotential embryonic stem cell line into a developing embryo to make a chimeric embryo; and (d) allowing said chimeric embryo to develop to term.
Claim 48
Independent Claim
97 words
A method for obtaining a transgenic animal having a plurality of cells containing at least one exogenous chromosome or exogenous chromosome fragment, said method comprising: (a) introducing at least one exogenous chromosome or exogenous chromosome fragment, each containing at least 100 kilobases of nucleic acids, into the heritable genetic material of an embryonic animal receipient cell; (b) generating a pluripotential embryonic stem cell line from said embryonic animal recipient cell; (c) transplanting said pluripotential embryonic stem cell line into a developing embryo to make a chimeric embryo; and (d) allowing said chimeric embryo to develop to term.
Claim 49
Independent Claim
83 words
A method for obtaining a transgenic animal having a plurality of cells containing at least one exogenous chromosome or exogenous chromosome fragment, said method comprising: (a) introducing at least one exogeneous chromosome or exogenous chromosome fragment, each containing at least 100 kilobases of nucleic acids, into the heritable genetic material of a pluripotential embryonic stem cell; (b) transplanting said pluripotential embryonic stem cell line into a developing embryo to make a chimeric embryo; and (c) allowing said chimeric embryo to develop to term.
Claim 50
Independent Claim
83 words
A method for obtaining a transgenic animal having a plurality of cells containing at least one exogenous chromosome or exogenous chromosome fragment, said method comprising: (a) introducing at least one exogeneous chromosome or exogenous chromosome fragment, each containing at least 100 kilobases of nucleic acids, into the heritable genetic material of a pluripotential embryonic stem cell; (b) transplanting said pluripotential embryonic stem cell line into a developing embryo to make a chimeric embryo; and (c) allowing said chimeric embryo to develop to term.
Claim 51
Independent Claim
82 words
A method for producing a human monoclonal antibody against an antigen, comprising: harvesting antibodies from a hybridoma cell line formed by fusing, with a myeloma cell, a B cell lymphocyte capable of producing a human antibody against an antigen, wherein said B cell lymphocyte is obtained from a transgenic animal; where said transgenic animal is an animal which comprises at least some cells containing at least one exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids.
Claim 52
Independent Claim
82 words
A method for producing a human monoclonal antibody against an antigen, comprising: harvesting antibodies from a hybridoma cell line formed by fusing, with a myeloma cell, a B cell lymphocyte capable of producing a human antibody against an antigen, wherein said B cell lymphocyte is obtained from a transgenic animal; where said transgenic animal is an animal which comprises at least some cells containing at least one exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids.
Claim 53
Independent Claim
98 words
A method for producing a hybridoma cell line which produces a human monoclonal antibody against an antigen, comprising: (a˝) immunizing a transgenic animal comprising at least some cells which contain at least one exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids; (b˝) harvesting B cell lymphocytes capable of producing antibody against said antigen from the lymph node, spleen or peripheral blood of said immunized transgenic animal; and (c˝) fusing said harvested B cell lymphocytes with a myeloma cell line to produce a hybridoma cell line which produces antibodies against said antigen.
Claim 54
Independent Claim
98 words
A method for producing a hybridoma cell line which produces a human monoclonal antibody against an antigen, comprising: (a˝) immunizing a transgenic animal comprising at least some cells which contain at least one exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids; (b˝) harvesting B cell lymphocytes capable of producing antibody against said antigen from the lymph node, spleen or peripheral blood of said immunized transgenic animal; and (c˝) fusing said harvested B cell lymphocytes with a myeloma cell line to produce a hybridoma cell line which produces antibodies against said antigen.
Claim 55
Dependent Claim
43 words
A method according to claim 38 or 39, in which said transgenic animal is a mouse and said myeloma cell is a murine myeloma cell or said transgenic animal is a rat and said myeloma cell is a murine or rat myeloma cell.
Claim 56
Dependent Claim
68 words
A monoclonal antibody produced by a method comprising: harvesting antibodies from a hybridoma cell line formed by fusing, with a myeloma cell, a B cell lymphocyte capable of producing an antibody against an antigen, wherein said B cell lymphocyte is obtained from a transgenic animal comprising at least some cells which contain an exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids.
Claim 57
Dependent Claim
68 words
A monoclonal antibody produced by a method comprising: harvesting antibodies from a hybridoma cell line formed by fusing, with a myeloma cell, a B cell lymphocyte capable of producing an antibody against an antigen, wherein said B cell lymphocyte is obtained from a transgenic animal comprising at least some cells which contain an exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids.
Claim 58
Dependent Claim
105 words
A hybridoma cell line produced by a method comprising: (a‴) immunizing, with an antigen, a transgenic animal comprising at least some cells which contain at least one exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids, and comprising genetic material encoding a human immunoglobulin complex or a portion thereof; (b‴) harvesting B cell lymphocytes capable of producing antibody against said antigen from the lymph node, spleen or peripheral blood of said immunized transgenic animal; and (c‴) fusing said harvested B cell lymphocytes with a myeloma cell line to produce a hybridoma cell line which produces antibodies against said antigen.
Claim 59
Dependent Claim
105 words
A hybridoma cell line produced by a method comprising: (a‴) immunizing, with an antigen, a transgenic animal comprising at least some cells which contain at least one exogenous chromosome or exogenous chromosome fragment, each comprising more than 100 kilobases of nucleic acids, and comprising genetic material encoding a human immunoglobulin complex or a portion thereof; (b‴) harvesting B cell lymphocytes capable of producing antibody against said antigen from the lymph node, spleen or peripheral blood of said immunized transgenic animal; and (c‴) fusing said harvested B cell lymphocytes with a myeloma cell line to produce a hybridoma cell line which produces antibodies against said antigen.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.