Patent EP0373203B1 successfully refreshed from Google Patents.
EP0373203B1
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Method and apparatus for analysing polynucleotide sequences
Assignee: Oxford Gene Technology Ltd
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Filing: 1989-05-02
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Granted: 1994-08-31
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Expires: 2009-05-02
Abstract
Abstract An apparatus is provided for analysing a polynucleotide. The apparatus includes: a support having an impermeable surface; porous material attached to the impermeable surface; and an array of oligonucleotides with predetermined sequences attached to the porous material. The array includes at least two defined cells, the sequence of the oligonucleotides of a first cell being different from the sequence of the oligonucleotides of a second cell, and the oligonucleotides being shorter than the polynucleotide.
Inventors
- 👤 Edwin Southern
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 15:40
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; B; B01; B01J; B01J19/00; B01J19/0046; C12Q1/6813; C12Q1/6827; C12Q1/6834; C12Q1/6837; C12Q1/6869; C12Q1/6874; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00317; B01J2219/00351; B01J2219/00364; B01J2219/00378; B01J2219/00385; B01J2219/00427; B01J2219/0043; B01J2219/00497; B01J2219/00527; B01J2219/00529; B01J2219/0054; B01J2219/00572; B01J2219/00574; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00608; B01J2219/00612; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00639; B01J2219/00644; B01J2219/00659; B01J2219/0068; B01J2219/00686; B01J2219/00689; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
89 words
A method of analyzing a polynucleotide sequence, by the use of a support to the surface of which is attached an array of the whole or a chosen part of a complete set of oligonucleotides of chosen lengths, the different oligonucleotides occupying separate cells of the array, which method comprises labelling the polynucleotide sequence or fragments thereof, applying the polynucleotide sequence or fragments thereof under hybridisation conditions to the array, and observing the location of the label on the surface associated with particular members of the set of oligonucleotides.
Claim 2
Dependent Claim
39 words
A method according to claim 1, applied to the study of differences between polynucleotide sequences, wherein the array is of the whole or a chosen part of the complete set of oligonucleotides of chosen lengths comprising the polynucleotide sequences.
Claim 3
Dependent Claim
20 words
A method as claimed in claim 2, wherein the array comprises one or more pairs of oligonucleotides of chosen lengths.
Claim 4
Dependent Claim
28 words
A method as claimed in claim 3, wherein the array comprises one or more pairs of oligonucleotides representing normal and mutant versions of a point mutation being studied.
Claim 5
Independent Claim
43 words
A method according to any one of claims 1 to 4, wherein the polynucleotide sequence is randomly degraded to form a mixture of oligomers of a chosen length, the mixture being thereafter labelled to form labelled material which is applied to the array.
Claim 6
Dependent Claim
14 words
A method as claimed in claim 5, wherein the oligomers are labelled with ³²P.
Claim 7
Independent Claim
25 words
A method as claimed in any one of claims 1 to 6, wherein the chosen length of the oligonucleotides is from 8 to 20 nucleotides.
Claim 8
Independent Claim
28 words
A method as claimed in any one of claims 1 to 7, wherein each oligonucleotide is bound to the support by a covalent link through a terminal nucleotide.
Claim 9
Independent Claim
22 words
A method as claimed in any one of claims 1 to 8, wherein the oligonucleotide at each cell has a defined sequence.
Claim 10
Dependent Claim
63 words
Apparatus suitable for analysing a polynucleotide sequence by the method of any one of claims 1 to 9 comprising a support and attached to a surface thereof an array of the whole or a chosen part of a complete set of oligonucleotides of chosen lengths, the different oligonucleotides occupying separate cells of the array and being capable of taking part in hybridisation reactions.
Claim 11
Dependent Claim
36 words
Apparatus as claimed in claim 10 for studying differences between polynucleotide sequences, wherein the array is of the whole or a chosen part of a complete set of oligonucleotides of chosen lengths comprising the polynucleotide sequences.
Claim 12
Dependent Claim
19 words
Apparatus as claimed in claim 11, wherein the array comprises one or more pairs of oligonucleotides of chosen lengths.
Claim 13
Dependent Claim
28 words
Apparatus as claimed in claim 12, wherein the array comprises one or more pairs of oligonucleotides representing normal and mutant versions of a point mutation to be studied.
Claim 14
Dependent Claim
66 words
Apparatus for determining the sequence of a polynucleotide comprising a support having attached to a surface thereof an array of different oligonucleotides with defined sequences, the oligonucleotides occupying cells of the array and being attached to the surface, wherein the defined sequence of an oligonucleotide of one cell of the array is different than the defined sequence of an oligonucleotide of another cell of the array.
Claim 15
Dependent Claim
48 words
Apparatus for analysing a polynucleotide, the apparatus comprising a support segregated into at least two defined cells, each cell having attached thereto oligonucleotides with known sequence, where the sequence of the oligonucleotides of a first cell is different than the sequence of the oligonucleotides of a different cell.
Claim 16
Dependent Claim
24 words
Apparatus as claimed in any one of claims 10 to 15, wherein the chosen length of the oligonucleotides is from 8 to 20 nucleotides.
Claim 17
Dependent Claim
26 words
Apparatus as claimed in any one of claims 10 to 16, wherein the surface of the support to which the oligonucleotides are attached is of glass.
Claim 18
Dependent Claim
27 words
Apparatus as claimed in any one of claims 10 to 17, wherein each oligonucleotide is bound to the support by a covalent link through a terminal nucleotide.
Claim 19
Dependent Claim
111 words
A method for generating, for the apparatus of claim 14, an array of oligonucleotides of chosen lengths within discrete cells of a support material comprising the steps of a) segregating a support material into discrete cell locations; b) coupling a nucleotide to a first set of cell locations; c) coupling a nucleotide to a second set of cell locations; d) coupling a nucleotide to a third set of cell locations; e) and continuing the sequence of coupling steps until the desired array has been generated, the coupling being effected at each location either to the surface of the support or to a nucleotide coupled in a previous step at that location.
Claim 20
Dependent Claim
19 words
The method of claim 19 wherein a microcomputer controlled plotter delivers the nucleotides to said sets of cell locations.
Claim 21
Dependent Claim
21 words
The method of claim 19 or claim 20 wherein the size of each discrete cell is between 10 and 100 µm.
Claim 22
Independent Claim
36 words
The method of any one of claims 19 to 21 further comprising the use of means for coupling said nucleotides to a particular set of discrete cell locations to the exclusion of other discrete cell locations.
Claim 23
Dependent Claim
11 words
The method of claim 22 wherein said means is a mask.
References Cited (Backward Citations)
No citation records indexed.
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