Patent EP0369861A2 successfully refreshed from Google Patents.
EP0369861A2
Expired / Lapsed
EP
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Normal human growth regulatory receptor for TGF beta
Assignee: Oncogen LP
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Filing: 1989-11-08
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Granted: —
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Expires: 2009-11-08
Abstract
Abstract Type III TGF-β receptor is identified in and purified from normal human embryonic palatal mesenchyme (HEPM) cells and the purified product characterized structurally and functionally. HEPM cells were found to express high levels of the type III TGF-β receptor and were found to significantly down-regulate two classes of TGF-β receptor binding site. Purification of the type III TGF-β receptor from solubilized HEPM cell membranes by affinity chromatography yielded a biologically active protein of about 205 kd which specifically binds both the recombinant and natural forms of TGF-β1 and TGF-β2, with affinity dissociation constants in the picomolar range.
Inventors
- 👤 Mario N. Lioubin
- 👤 Thomas J. Brown
- 👤 Anthony F. Purchio
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 13:06
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/71
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
31 words
A purified protein that: (a) has an amino-terminal amino acid sequence comprising: NH₂-Lys-Tyr-Tyr-Asp-Lys-Asp-Tyr (b) has a molecular weight of about 100,000 to about 220,000 daltons (c) is a receptor for TGF-β
Claim 2
Dependent Claim
31 words
A purified protein that: (a) has an amino-terminal amino acid sequence comprising: NH₂-Lys-Tyr-Tyr-Asp-Lys-Asp-Tyr (b) has a molecular weight of about 100,000 to about 220,000 daltons (c) is a receptor for TGF-β
Claim 3
Dependent Claim
6 words
A glycosylated protein of claim 1.
Claim 4
Dependent Claim
6 words
An un-glycosylated protein of claim 1.
Claim 5
Dependent Claim
15 words
The protein of claim 1 or a peptide or fragment thereof that specifically binds TGF-β.
Claim 6
Dependent Claim
12 words
The protein of claim 2 in which the TGF-β is natural TGF-β1.
Claim 7
Dependent Claim
12 words
The protein of claim 2 in which the TGF-β is recombinant TGF-β1.
Claim 8
Dependent Claim
12 words
The protein of claim 2 in which the TGF-β is natural TGF-β2.
Claim 9
Dependent Claim
12 words
The protein of claim 2 in which the TGF-β is recombinant TGF-β2.
Claim 10
Dependent Claim
19 words
The protein of claim 6 having a dissociation constant of about 1 X 10⁻¹²M to about 1 X 10⁻⁹M.
Claim 11
Dependent Claim
31 words
Purified type III TGF-β receptor, obtained by the method comprising: (a) solubilizing membranes from cultured HEPM cells (b) affinity purifying type III TGF-β receptor there from using TGF-β as the ligand.
Claim 12
Dependent Claim
31 words
Purified type III TGF-β receptor, obtained by the method comprising: (a) solubilizing membranes from cultured HEPM cells (b) affinity purifying type III TGF-β receptor there from using TGF-β as the ligand.
Claim 13
Dependent Claim
20 words
A purified protein that: (a) has a molecular weight of about 100,000 to about 220,000 daltons (b) specifically binds TGF-β.
Claim 14
Dependent Claim
20 words
A purified protein that: (a) has a molecular weight of about 100,000 to about 220,000 daltons (b) specifically binds TGF-β.
Claim 15
Dependent Claim
12 words
The protein of claim 11 in which the TGF-β is natural TGF-β1.
Claim 16
Dependent Claim
12 words
The protein of claim 11 in which the TGF-β is recombinant TGF-β1.
Claim 17
Dependent Claim
12 words
The protein of claim 11 in which the TGF-β is natural TGF-β2.
Claim 18
Dependent Claim
12 words
The protein of claim 11 in which the TGF-β is recombinant TGF-β2.
Claim 19
Dependent Claim
19 words
The protein of claim 13 having a dissociation constant of about 1 X 10⁻¹²M to about 1 X 10⁻⁹M.
References Cited (Backward Citations)
No citation records indexed.
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