Patent EP0412796A2 successfully refreshed from Google Patents.
Patent EP0360205A2 successfully refreshed from Google Patents.
EP0360205A2
Expired / Lapsed
EP
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Method for the induction and activation of cytotoxic T cells
Assignee: Ajinomoto Co Inc
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Filing: 1989-09-19
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Granted: —
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Expires: 2009-09-19
Abstract
Abstract The use of a serum-free medium for the induction of human self cancer-specific cytotoxic T cells (CTL) is disclosed.
Inventors
- 👤 Shigeyoshi Fujimoto
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 20:16
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0634; C12N5/0636; A; A61; A61K; A61K39/00; A61K2039/51; A61K2039/515; A61K2039/5158; C12N2500/00; C12N2500/05; C12N2500/10; C12N2500/20; C12N2500/24; C12N2500/25; C12N2500/90; C12N2500/92
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
30 words
In a method for the induction and activation of cytotoxic T cells, the improvement comprising using a serum-free medium to cause the induction of human self cancer-specific cytotoxic T cells.
Claim 2
Dependent Claim
30 words
In a method for the induction and activation of cytotoxic T cells, the improvement comprising using a serum-free medium to cause the induction of human self cancer-specific cytotoxic T cells.
Claim 3
Dependent Claim
13 words
The method of Claim 1, comprising adding an immunoactivator to said serum-free medium.
Claim 4
Dependent Claim
14 words
The method of Claim 1, comprising adding a growth factor to said serum-free medium.
Claim 5
Dependent Claim
30 words
The method of Claim 3, wherein said growth factor is at least one member selected from the group consisting of insulin, transferin, steroid hormones, ethanol amine, and human serum albumin.
Claim 6
Dependent Claim
18 words
The method of Claim 4, comprising using insulin of human, bovine or swine origin as the insulin component.
Claim 7
Dependent Claim
11 words
The method of Claim 4, comprising using transferin of human origin.
Claim 8
Dependent Claim
17 words
The method of Claim 4, comprising using recombinant human insulin, recombinant human transferin or recombinant human albumin.
Claim 9
Independent Claim
117 words
A method for the induction and activation of cytotoxic T cells, comprising: (i) combining (ia) self cancer cells unable to divide and proliferate with (ib) lymphocytes isolated from the peripheral blood of a patient, and (ic) a serum-free medium, to obtain a composition having a cell density of from 3 to 5 x 10⁶ ml⁻¹ and from 1 to 5 x 10⁴ ml⁻¹, respectively; (ii) culturing said composition for 2 to 4 days under an atmosphere of carbon dioxide; (iii) adding interleukin-2 to the medium at a concentration of 25 to 75 ng ml⁻¹; (iv) culturing said medium for an additional 2 to 4 days; and (v) obtaining induced inactivated CTL specific to said self cancer cells.
Claim 10
Independent Claim
117 words
A method for the induction and activation of cytotoxic T cells, comprising: (i) combining (ia) self cancer cells unable to divide and proliferate with (ib) lymphocytes isolated from the peripheral blood of a patient, and (ic) a serum-free medium, to obtain a composition having a cell density of from 3 to 5 x 10⁶ ml⁻¹ and from 1 to 5 x 10⁴ ml⁻¹, respectively; (ii) culturing said composition for 2 to 4 days under an atmosphere of carbon dioxide; (iii) adding interleukin-2 to the medium at a concentration of 25 to 75 ng ml⁻¹; (iv) culturing said medium for an additional 2 to 4 days; and (v) obtaining induced inactivated CTL specific to said self cancer cells.
Claim 11
Dependent Claim
30 words
The method of Claim 8, comprising using, instead of said self cancer cells, homologous cancer cells in which at least a part of the human leukocyte antigen type is identical.
Claim 12
Dependent Claim
25 words
A kit for the induction and activation of cytotoxic T cells comprising a serum-free medium and a means for culturing cells in a suspension culture.
Claim 13
Dependent Claim
25 words
A kit for the induction and activation of cytotoxic T cells comprising a serum-free medium and a means for culturing cells in a suspension culture.
Claim 14
Dependent Claim
13 words
The kit of Claim 10, wherein said serum-free medium comprises a growth factor.
Claim 15
Dependent Claim
30 words
The kit of Claim 11, wherein said growth factor is at least one member selected from the group consisting of insulin, transferin, steroid hormones, ethanol amine, and human serum albumin.
Claim 16
Dependent Claim
17 words
The kit of Claim 12, comprising insulin of human, bovine or swine origin as said insulin component.
Claim 17
Dependent Claim
10 words
The kit of Claim 12, comprising transferin of human origin.
Claim 18
Dependent Claim
9 words
The kit of Claim 12, comprising recombinant human insulin.
Claim 19
Dependent Claim
9 words
The kit of Claim 12, comprising recombinant human transferin.
Claim 20
Dependent Claim
9 words
The kit of Claim 12, comprising recombinant human albumin.
References Cited (Backward Citations)
No citation records indexed.
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