Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
147 words
A method for the determination of an amount of an antigen or antibody comprising loading an antibody or antigen on insoluble carrier particles, reacting the loaded antibody or antigen with the antigen, antibody or their mixture in a liquid medium and measuring the degree in change of reaction product associated with the progress of the said reaction, characterized in that the antigen or antibody in the liquid medium is qualitatively or quantitatively measured by carrying out the said reaction using both insoluble carrier particles containing a magnetic substance and those not containing any magnetic substance as the above-mentioned carrier particles, subsequently by applying a magnetic field to the reaction mixture in order to separate aggregated particles comprising the magnetic substance-containing insoluble carrier particles and unreacted magnetic substance-containing insoluble carrier particles from the reaction mixture, and by detecting the remaining insoluble carrier particles not containing the magnetic substance.
Claim 2
Independent Claim
147 words
A method for the determination of an amount of an antigen or antibody comprising loading an antibody or antigen on insoluble carrier particles, reacting the loaded antibody or antigen with the antigen, antibody or their mixture in a liquid medium and measuring the degree in change of reaction product associated with the progress of the said reaction, characterized in that the antigen or antibody in the liquid medium is qualitatively or quantitatively measured by carrying out the said reaction using both insoluble carrier particles containing a magnetic substance and those not containing any magnetic substance as the above-mentioned carrier particles, subsequently by applying a magnetic field to the reaction mixture in order to separate aggregated particles comprising the magnetic substance-containing insoluble carrier particles and unreacted magnetic substance-containing insoluble carrier particles from the reaction mixture, and by detecting the remaining insoluble carrier particles not containing the magnetic substance.
Claim 3
Independent Claim
208 words
A method for the determination of an amount of an antigen or antibody wherein the antigen or antibody is qualitatively or quantitatively measured by allowing a suspension of insoluble carrier particles containing a magnetic substance or of those not containing any magnetic substance, which particles have been sensitized with an antibody for the antigen to be measured or with an antigen for the antibody to be measured, to react with the antigen or antibody to be measured in an aqueous medium for a certain time insufficient to attain the equilibrium of an antigen-antibody reaction, then by adding to the reaction mixture a suspension of the insoluble carrier particles not containing the magnetic substance or of those containing the magnetic substance, which particles have been sensitized with the antibody for the antigen to be measured or with the antigen for the antibody to be measured, by incubating the reaction mixture until the antigen-antibody reaction reaches a state of equilibrium, whereupon by applying a magnetic field to the reaction mixture in order to separate unreacted particles containing the magnetic substance and aggregated particles comprising the magnetic substance from the insoluble carrier particles not containing the magnetic substance, and by detecting the remaining insoluble carrier particles not containing the magnetic substance.
Claim 4
Independent Claim
208 words
A method for the determination of an amount of an antigen or antibody wherein the antigen or antibody is qualitatively or quantitatively measured by allowing a suspension of insoluble carrier particles containing a magnetic substance or of those not containing any magnetic substance, which particles have been sensitized with an antibody for the antigen to be measured or with an antigen for the antibody to be measured, to react with the antigen or antibody to be measured in an aqueous medium for a certain time insufficient to attain the equilibrium of an antigen-antibody reaction, then by adding to the reaction mixture a suspension of the insoluble carrier particles not containing the magnetic substance or of those containing the magnetic substance, which particles have been sensitized with the antibody for the antigen to be measured or with the antigen for the antibody to be measured, by incubating the reaction mixture until the antigen-antibody reaction reaches a state of equilibrium, whereupon by applying a magnetic field to the reaction mixture in order to separate unreacted particles containing the magnetic substance and aggregated particles comprising the magnetic substance from the insoluble carrier particles not containing the magnetic substance, and by detecting the remaining insoluble carrier particles not containing the magnetic substance.
Claim 5
Dependent Claim
36 words
A method for the determination of an amount of an antigen or antibody according to Claim 1 or 2 wherein the insoluble carrier particles containing iron and/or magnetic iron oxide as the magnetic substance are used.
Claim 6
Dependent Claim
31 words
A method for the determination of an amount of an antigen or antibody according to Claim 1, 2 or 3 wherein organic polymer material is used for the insoluble carrier particles.
Claim 7
Dependent Claim
31 words
A method for the determination of an amount of an antigen or antibody according to Claim 1, 2 or 3 wherein inorganic fine powder is used for the insoluble carrier particles.
Claim 8
Dependent Claim
30 words
A method for the determination of an amount of an antigen or antibody according to Claim 4 wherein polystyrene latex or styrene-butadiene copolymer is used for the insoluble carrier particles.
Claim 9
Dependent Claim
37 words
A method for the determination of an amount of an antigen or antibody according to Claim 5 wherein ceramic fine particles, silica, alumina, silica-alumina charcoal powder or metal fine powder is used for the insoluble carrier particles.
Claim 10
Dependent Claim
45 words
A method for the determination of an amount of an antigen or antibody according to Claim 1 or 2 wherein the insoluble carrier particles containing the magnetic substance and those not containing the magnetic substance are combined at a ratio of between 1:20 and 20:1.
Claim 11
Dependent Claim
49 words
A method for the determination of an amount of an antigen or antibody according to Claim 1 or 2 wherein the amount of the antigen or antibody in a specimen is qualitatively decided by.visual detection of the amount of the remaining insoluble carrier particles not containing the magnetic substance.
Claim 12
Dependent Claim
37 words
A method for the determination of an amount of an antigen or antibody according to Claim 1 or 2 wherein organic polymer particles comprising dye are used as the insoluble carrier particles not containing the magnetic substance.
Claim 13
Dependent Claim
31 words
A method for the determination of an amount of an antigen or antibody according to Claim 10 wherein two or more kinds of the organic polymer particles comprising dye are used.