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EP0356257A2
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EP
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Processes for the lyophilization of cells including lyophilization media, and methods of reconstituting lyophilized cells
Assignee: Terumo BCT Inc
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Filing: 1989-08-25
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Granted: —
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Expires: 2009-08-25
Abstract
Abstract A process and medium are disclosed for the lyophilization of cells which comprises the use of solutions including monosaccharide hexoses and pentoses, and/or bicompatible amphipathic polymers to permit the reconstitution of viable cells.
Inventors
- 👤 Raymond Paul Goodrich
- 👤 Christine Marie Williams
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 18:08
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
57 words
A process for the lyophilization of cells having a cell membrane, comprising immersing a plurality of cells in a water solution which includes a monosaccharide, which is capable of permeating the membrane of the cells, in a concentration of from about 7.0 to 37.5%; freezing the solution; and drying the frozen cells by sublimation of the water.
Claim 2
Dependent Claim
57 words
A process for the lyophilization of cells having a cell membrane, comprising immersing a plurality of cells in a water solution which includes a monosaccharide, which is capable of permeating the membrane of the cells, in a concentration of from about 7.0 to 37.5%; freezing the solution; and drying the frozen cells by sublimation of the water.
Claim 3
Dependent Claim
78 words
A process for the lyophilization of cells, comprising: immersing a plurality of cells in a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7% up to saturation in the solution; freezing the solution; and drying the cells by sublimation of the water.
Claim 4
Dependent Claim
78 words
A process for the lyophilization of cells, comprising: immersing a plurality of cells in a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7% up to saturation in the solution; freezing the solution; and drying the cells by sublimation of the water.
Claim 5
Dependent Claim
23 words
A process as claimed in claim 1 or claim 2 wherein the monosaccharide is selected from the group consisting of pentoses and hexoses.
Claim 6
Dependent Claim
28 words
A process as claimed in any one of claims 1 to 3 wherein the monosaccharide is selected from the group consisting of xylose, glucose, ribose, mannose and fructose.
Claim 7
Dependent Claim
26 words
A process as claimed in any one of claims 1 to 4, wherein the monosaccharide is present in the solution in a concentration of about 23%.
Claim 8
Dependent Claim
17 words
A process as claimed in any one of claims 2 to 5 wherein said polymer is amphipathic.
Claim 9
Dependent Claim
28 words
A process as claimed in any one of claims 2 to 6 wherein said polymer has a molecular weight in the range of about 5K to about 80K.
Claim 10
Dependent Claim
25 words
A process as claimed in any one of claims 2 to 7 wherein the polymer is selected from the group consisting of polyvinylpyrrolidone and dextran.
Claim 11
Dependent Claim
12 words
A process as claimed in claim 8 wherein the polymer is polyvinylpyrrolidone.
Claim 12
Independent Claim
54 words
A medium for the lyophilization of cells, comprising a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7%.
Claim 13
Independent Claim
54 words
A medium for the lyophilization of cells, comprising a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7%.
Claim 14
Dependent Claim
20 words
A medium as claimed in claim 10 modified by the specific feature of any one of claims 3 to 9.
Claim 15
Independent Claim
27 words
A method of reconstituting lyophilized cells comprising the step of contacting said cells with an aqueous solution containing a carbohydrate in a concentration of at least 1%.
Claim 16
Independent Claim
27 words
A method of reconstituting lyophilized cells comprising the step of contacting said cells with an aqueous solution containing a carbohydrate in a concentration of at least 1%.
Claim 17
Dependent Claim
18 words
A method according to claim 12 wherein said carbohydrate concentration is in the range of 1 to 30%.
Claim 18
Dependent Claim
19 words
A method according to claim 12 or claim 13 wherein said carbohydrate is a monosaccharide, disaccharide, or mixture thereof.
Claim 19
Dependent Claim
21 words
A method according to claim 14 wherein said carbohydrate is selected from the group consisting of glucose, mannose, trehalose and sucrose.
Claim 20
Dependent Claim
11 words
A method according to claim 15 wherein said carbohydrate comprises sucrose.
Claim 21
Independent Claim
39 words
A method according to any one of claims 12 to 16 wherein said solution additionally contains a polymer having a molecular weight of from about 1K to about 360K in a concentration of up to about 20% by weight.
Claim 22
Independent Claim
41 words
A method of reconstituting lyophilized cells comprising the step of contacting said cells with an aqueous solution containing a polymer having a molecular weight of from about 1K to about 360K in a concentration of up to about 20% by weight.
Claim 23
Independent Claim
41 words
A method of reconstituting lyophilized cells comprising the step of contacting said cells with an aqueous solution containing a polymer having a molecular weight of from about 1K to about 360K in a concentration of up to about 20% by weight.
Claim 24
Dependent Claim
24 words
A method as claimed in claim 17 or claim 18 modified by the specific features of any one of claims 6, 7 or 9.
Claim 25
Independent Claim
20 words
A method according to any one of claims 12 to 19 wherein said solution is from 20° to about 40°C.
Claim 26
Dependent Claim
42 words
A process as claimed in any one of claims 1 to 9, a medium as claimed in claim 10 or claim 11, or a method as claimed in any one of claims 12 to 20 wherein the cells are red blood cells.
References Cited (Backward Citations)
No citation records indexed.
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