Patent EP0350004A2 successfully refreshed from Google Patents.
EP0350004A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for determination of pyrogen content
Assignee: Tanabe Pharma Corp
•
Filing: 1989-07-05
•
Granted: —
•
Expires: 2009-07-05
Abstract
Abstract A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound bonded to a water-insoluble carrier directly or through a spacer, and after or without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by means of Limulus method.
Inventors
- 👤 Tetsuya Tosa
- 👤 Tadashi Sato
- 👤 Taizo Watanabe
- 👤 Satoshi Minobe
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 02:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/579
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
57 words
A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound bonded to a water-insoluble carrier directly or through a spacer, and after or without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by means of Limulus method.
Claim 2
Independent Claim
57 words
A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound bonded to a water-insoluble carrier directly or through a spacer, and after or without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by means of Limulus method.
Claim 3
Dependent Claim
31 words
A method according to claim 1, wherein the adsorbent is histidine, histamine, urocanic acid, uracil, orotic acid, cytosine, 5-methylcytosine, 2-amino-4,6-dimethylpyrimidine, adenine or 6,9-diamino-2-ethoxyacridine bonded to a water-insoluble polysaccharide through a spacer.
Claim 4
Dependent Claim
30 words
A method according to claim 2, wherein the water-insoluble polysaccharide is a member selected from the group consisting of celluloses, agarose, cross-linked dextran, their aminobenzylated derivatives and their carboxyalkylated derivatives.
Claim 5
Dependent Claim
36 words
A method according to claim 2, wherein the spacer is a compound of formula: NH₂(CH₂) n NH₂, NH₂(CH₂) n COOH, NH₂(CH₂) n OH, or HOOC(CH₂) n COOH wherein n is an integer of 1 to 12.
Claim 6
Dependent Claim
17 words
A method according to claim 1, wherein the specimen is a water-containing solution or an aqueous solution.
Claim 7
Dependent Claim
54 words
A method according to claim 1, wherein the adsorbent is used in an amount of 3 to 100 mg per 1 ml of a specimen and the adsorption is carried out under conditions of a temperature of 4 to 40°C, pH of 4 to 8 and an ionic strength of not more than 0.1.
Claim 8
Dependent Claim
33 words
A method according to claim 1, wherein the adsorbent is a nitrogen-containing heterocyclic compound bonded to a water-insoluble polysaccharide through an alkylenediamine by epichlorohydrin, and said nitrogen-containing heterocyclic compound being histidine or histamine.
Claim 9
Independent Claim
14 words
A method according to cliam 7, wherein said water-insoluble polysaccharide is cellulose or agarose.
Claim 10
Independent Claim
14 words
A method according to cliam 7, wherein said water-insoluble polysaccharide is cellulose or agarose.
Claim 11
Independent Claim
53 words
A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent (said adsorbent being histidine bonded to a water-insoluble polysaccharide through an alkylenediamine by epichlorohydrin), and without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by means of Limulus method.
Claim 12
Independent Claim
53 words
A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent (said adsorbent being histidine bonded to a water-insoluble polysaccharide through an alkylenediamine by epichlorohydrin), and without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by means of Limulus method.
Claim 13
Independent Claim
53 words
A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent (said adsorbent being histidine bonded to a water-insoluble polysaccharide through an alkylenediamine by epichlorohydrin), and after eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by means of Limulus method.
Claim 14
Independent Claim
53 words
A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent (said adsorbent being histidine bonded to a water-insoluble polysaccharide through an alkylenediamine by epichlorohydrin), and after eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by means of Limulus method.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.