Patent EP0342447A2 successfully refreshed from Google Patents.
EP0342447A2
Expired / Lapsed
EP
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Direct measuring assay strip
Assignee: ChemTrak Inc
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Filing: 1989-05-05
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Granted: —
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Expires: 2009-05-05
Abstract
Abstract Methods and compositions are provided for measuring an analyte without instrumentation. The device employs a stripstick which may include as its components, a discontinuous flow path, which is made continuous after transfer of the sample, means for automatically metering the volume of a sample, and means for providing a sharply delineated color front. The method finds particular use where a limited amount of substrate is provided for an enzyme on a sample pad.
Inventors
- 👤 Michael P. Allen
- 👤 Robert B. Shibuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 05:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/52; G01N33/525; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/54; C12Q1/60; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
137 words
A dipstick measuring device comprising: in the direction of eluent flow, a first bibulous bridging strip extending from the immersion end of said device to a sample pad site; a sample pad; a second bibulous bridging strip extending from said sample pad site to a measurement region fluid receiving site, said second strip in fluid communication with said sample pad; and an extended bibulous measurement strip in fluid communication with said second bridging strip and impregnated with a first member of a signal producing system, which upon reaction with a second member of said signal producing system produces a detectable signal defining a boundary on said measurement strip; and means for inhibiting fluid communication between said sample pad and said first and second bridging members prior to said measuring and for permitting fluid communication during said measuring.
Claim 2
Dependent Claim
137 words
A dipstick measuring device comprising: in the direction of eluent flow, a first bibulous bridging strip extending from the immersion end of said device to a sample pad site; a sample pad; a second bibulous bridging strip extending from said sample pad site to a measurement region fluid receiving site, said second strip in fluid communication with said sample pad; and an extended bibulous measurement strip in fluid communication with said second bridging strip and impregnated with a first member of a signal producing system, which upon reaction with a second member of said signal producing system produces a detectable signal defining a boundary on said measurement strip; and means for inhibiting fluid communication between said sample pad and said first and second bridging members prior to said measuring and for permitting fluid communication during said measuring.
Claim 3
Dependent Claim
22 words
A device according to Claim 1, wherein said first and second bridging strips overlap said sample pad without contact between said strips.
Claim 4
Dependent Claim
21 words
A device according to Claim 1, wherein said signal producing system comprises at least one enzyme impregnated in said sample pad.
Claim 5
Dependent Claim
12 words
A device according to Claim 3, wherein said enzyme is an oxidase.
Claim 6
Dependent Claim
177 words
A dipstick measuring device comprising: in the direction of eluent flow, a first bibulous bridging strip extending from the immersion end of said device to a sample pad site; a sample pad at said sample pad site in fluid communication with said first bridging strip; a second bibulous bridging strip extending from said sample pad site to a measurement region fluid receiving site, said second strip in fluid communication with said sample pad; and an extended bibulous measurement strip in fluid communication with said second bridging strip and impregnated with a first member of a signal producing system, which upon reaction with a second member of said signal producing system produces a detectable signal defining a boundary on said measurement strip; and in addition an inert protective barrier separating said sample pad from fluid communication with said first and second bridging members; and a spanning bibulous strip extending from said immersion end to said measurement strip in fluid communication with said measurement strip; and wherein said second bridging strip has a region narrower than said spanning strip.
Claim 7
Dependent Claim
177 words
A dipstick measuring device comprising: in the direction of eluent flow, a first bibulous bridging strip extending from the immersion end of said device to a sample pad site; a sample pad at said sample pad site in fluid communication with said first bridging strip; a second bibulous bridging strip extending from said sample pad site to a measurement region fluid receiving site, said second strip in fluid communication with said sample pad; and an extended bibulous measurement strip in fluid communication with said second bridging strip and impregnated with a first member of a signal producing system, which upon reaction with a second member of said signal producing system produces a detectable signal defining a boundary on said measurement strip; and in addition an inert protective barrier separating said sample pad from fluid communication with said first and second bridging members; and a spanning bibulous strip extending from said immersion end to said measurement strip in fluid communication with said measurement strip; and wherein said second bridging strip has a region narrower than said spanning strip.
Claim 8
Dependent Claim
26 words
A device according to Claim 5, wherein said spanning bibulous strip intervenes between and provides fluid communication between said second bridging strip and said measurement strip.
Claim 9
Dependent Claim
174 words
A dipstick measuring device comprising: in the direction of eluent flow, a first bibulous bridging strip extending from the immersion end of said device to a sample pad site; a sample pad at said sample pad site in fluid communication with said first bridging strip; a second bibulous bridging strip extending from said sample pad site to a measurement region fluid receiving site, said second strip in fluid communication with said sample pad; and an extended bibulous measurement strip in fluid communication with said second bridging strip and impregnated with a first member of a signal producing system, which upon reaction with a second member of said signal producing system produces a detectable signal defining a boundary on said measurement strip; and a metering device comprising a porous non-wetting member in fluid receiving contact with said sample pad, an absorbant layer backing for receiving fluid from said sample pad, covered with an inert film and providing an inert protective barrier separating said sample pad from fluid communication with said first and second bridging members.
Claim 10
Dependent Claim
174 words
A dipstick measuring device comprising: in the direction of eluent flow, a first bibulous bridging strip extending from the immersion end of said device to a sample pad site; a sample pad at said sample pad site in fluid communication with said first bridging strip; a second bibulous bridging strip extending from said sample pad site to a measurement region fluid receiving site, said second strip in fluid communication with said sample pad; and an extended bibulous measurement strip in fluid communication with said second bridging strip and impregnated with a first member of a signal producing system, which upon reaction with a second member of said signal producing system produces a detectable signal defining a boundary on said measurement strip; and a metering device comprising a porous non-wetting member in fluid receiving contact with said sample pad, an absorbant layer backing for receiving fluid from said sample pad, covered with an inert film and providing an inert protective barrier separating said sample pad from fluid communication with said first and second bridging members.
Claim 11
Independent Claim
197 words
A method for measuring the presence of an analyte in a sample, employing a device comprising a first bridging bibulous member for transferring eluent from a fluid source to a sample pad, a second bridging member for transferring eluent fluid from said sample pad to a measuring extended bibulous strip impregnated with a second member of a signal producing system, said method comprising: impregnating a sample pad with an assay medium resulting in the presence of a first member of a signal producing system in said sample pad in an amount related to the amount of analyte in said sample, while preventing transport of said assay medium to said bridging bibulous members; directing an eluent stream from an eluent source to said sample pad to transfer said first member from said pad to said measuring strip through said second bridging member; directing said first member containing eluent through said measuring strip, whereby said first and second members of said signal producing system and any additional members of said signal producing system react to produce a detectable boundary on said measuring strip, wherein the extent of said boundary is related to the amount of analyte in said sample.
Claim 12
Independent Claim
197 words
A method for measuring the presence of an analyte in a sample, employing a device comprising a first bridging bibulous member for transferring eluent from a fluid source to a sample pad, a second bridging member for transferring eluent fluid from said sample pad to a measuring extended bibulous strip impregnated with a second member of a signal producing system, said method comprising: impregnating a sample pad with an assay medium resulting in the presence of a first member of a signal producing system in said sample pad in an amount related to the amount of analyte in said sample, while preventing transport of said assay medium to said bridging bibulous members; directing an eluent stream from an eluent source to said sample pad to transfer said first member from said pad to said measuring strip through said second bridging member; directing said first member containing eluent through said measuring strip, whereby said first and second members of said signal producing system and any additional members of said signal producing system react to produce a detectable boundary on said measuring strip, wherein the extent of said boundary is related to the amount of analyte in said sample.
Claim 13
Dependent Claim
58 words
A method according to Claim 8, further comprising; directing a diverted stream directly from said eluent source to said measuring member, wherein said diverted stream extends the width of said measuring member and said first member containing eluent stream is narrower than the width of said measuring member and is directed to the middle of said measuring member.
Claim 14
Dependent Claim
41 words
A method according to Claim 8, whereby said signal producing system comprises an oxidase covalently immobilized in said sample pad, a dialkylaniline covalently immobilized in said measuring strip, a hydrazine impregnated in said measuring strip and a peroxidase in said eluent.
Claim 15
Dependent Claim
47 words
A method according to Claim 16, wherein said oxidase is glucose oxidase, alcohol oxidase or cholesterol oxidase, and said analyte is glucose, ethanol or cholesterol, respectively, with the proviso that when said analyte is cholesterol, said signal producing system firther comprises cholesterol impregnated in said sample pad.
Claim 16
Dependent Claim
20 words
A kit comprising a device according to Claim 1, members of said signal producing system, and a sample metering device.
References Cited (Backward Citations)
No citation records indexed.
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