Patent EP0339980A1 successfully refreshed from Google Patents.
EP0339980A1
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EP
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Magnetic micro-particles, method and apparatus for collecting specimens for use in labelling immune reactions, and method and device for preparing specimens
Assignee: NTT Inc
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Filing: 1989-04-26
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Granted: —
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Expires: 2009-04-26
Abstract
Abstract A laser magnetic immunoassay method, in which a gradient magnetic field is applied to a solution containing a mixture of unreacted magnetic-labeled body, a specimen and a magnetic-labeled immunocomplex which is a complex between the specimen and the magnetic-labeled body after antigen-antibody reaction, and the magnetic-labeled immunocomplex in the solution is distinguished and measured in situ by means of difference in magnetophoretic ability between the unreacted magnetic-labeled body and immunocomplex. measured by radiating laser beam to the solution and receiving and analyzing output light. therefrom. Also, described are a laser magnetic immunoassay apparatus, comprising agradient magnetic field generating device for generating gradient magnetic field, and a magnetophoretic tube placed inside the slanting magnetic field generating device adapted to practice the laser magnetic immunoassay above., and a superparamagnetic-labeled body and a method for the manufacture thereof.
Inventors
- 👤 Koichi Fujiwara
- 👤 Hiroko Mizutani
- 👤 Hiromichi Mizutani
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 17:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N35/00; G01N2035/00465; G01N2035/00534; G01N35/10; G01N2035/1027; G01N2035/1048; G01N2035/1055; G01N2474/00; G01N2474/20; G01N35/0098; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/971; Y10S436/00; Y10S436/806; Y10S436/824
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
74 words
A method for collecting a specimen, comprising subjecting a specimen and a magnetic-labeled antibody composed of a magnetic micro-particle and an antibody fixed to the micro-particle to antigen-antibody reaction to form an immunocomplex which is a complex of the specimen and the magnetic micro-particle, performing local concentration of the immunocomplex to a predetermined position by applying an external magnetic field, preferably gradient magnetic field, and collecting the immunocomplex at the position of local concentration.
Claim 2
Independent Claim
74 words
A method for collecting a specimen, comprising subjecting a specimen and a magnetic-labeled antibody composed of a magnetic micro-particle and an antibody fixed to the micro-particle to antigen-antibody reaction to form an immunocomplex which is a complex of the specimen and the magnetic micro-particle, performing local concentration of the immunocomplex to a predetermined position by applying an external magnetic field, preferably gradient magnetic field, and collecting the immunocomplex at the position of local concentration.
Claim 3
Dependent Claim
46 words
The method as claimed in Claim 1, wherein the specimen is fixed to a non-magnetic particle having a mass greater than the magnetic-labeled antibody prior to subjecting the specimen and the magnetic-labeled antibody to antigen-antibody reaction, and unreacted magnetic-labeled antibody is separated and removed by centrifugation.
Claim 4
Independent Claim
111 words
A device for collecting a specimen, comprising a test vessel for containing a solution containing an immunocomplex, which is a complex as a result of antigen-antibody reaction between a specimen and a magnetic-labeled antibody composed of a magnetic micro-particle and an antibody fixed to the micro-particle, an external magnetic field generating device for generating generating magnetic field, preferably gradient magnetic field and applying it to the solution in the test vessel to effect local concentration of the immunocomplex to a predetermined position, a magnetic member of collecting the immunocomplex at the position of local concentration, and a moving mechanism for achieving relative movement between the magnetic member and the test vessel.
Claim 5
Independent Claim
111 words
A device for collecting a specimen, comprising a test vessel for containing a solution containing an immunocomplex, which is a complex as a result of antigen-antibody reaction between a specimen and a magnetic-labeled antibody composed of a magnetic micro-particle and an antibody fixed to the micro-particle, an external magnetic field generating device for generating generating magnetic field, preferably gradient magnetic field and applying it to the solution in the test vessel to effect local concentration of the immunocomplex to a predetermined position, a magnetic member of collecting the immunocomplex at the position of local concentration, and a moving mechanism for achieving relative movement between the magnetic member and the test vessel.
Claim 6
Independent Claim
75 words
A method for preparing a specimen, comprising a first step of magnetically labeling a specimen to form a magnetic-labeled specimen, a second step of applying gradient magnetic field to the magnetic-labeled specimen to guide the specimen onto the surface of a grid for use in electron microscopic observation, and fixing the specimen on the grid, and a third step of subjecting the magnetic-labeled specimen on the grid to negative staining in the gradient magnetic field.
Claim 7
Independent Claim
75 words
A method for preparing a specimen, comprising a first step of magnetically labeling a specimen to form a magnetic-labeled specimen, a second step of applying gradient magnetic field to the magnetic-labeled specimen to guide the specimen onto the surface of a grid for use in electron microscopic observation, and fixing the specimen on the grid, and a third step of subjecting the magnetic-labeled specimen on the grid to negative staining in the gradient magnetic field.
Claim 8
Dependent Claim
34 words
The method as claimed in Claim 4, further comprising adding an antibody capable of reacting specifically with the specimen to a suspension containing the specimen to coagulate the specimen, prior to the magnetic labeling.
Claim 9
Dependent Claim
53 words
The method as claimed in Claim 4, further comprising applying gradient magnetic field to a suspension containing the magnetic-labeled specimen to locally concentrate the magnetic-labeled specimen to a position of local concentration on the surface of the suspension, inserting a thin tube to the position of local concentration, and recovering the magnetic-labeled specimen.
Claim 10
Dependent Claim
46 words
The method as claimed in Claim 4, further comprising holding the grid on a detachable film, and applying gradient magnetic field through the film from the side opposite to that on which the grid is held such that the position of the grid is the highest.
Claim 11
Independent Claim
79 words
A device for preparing a specimen, comprising a vessel containing a liquid containing a magnetic-labeled immunocomplex composed of a specimen and a magnetic-micro-particle obtainable by subjecting the specimen and the magnetic micro-particle bound thereto, a local concentration mechanism for locally concentrating the immunocomplex to a predetermined position, a recovery mechanism for recovering the specimen, and a guide mechanism for guiding the specimen onto the surface of a grid for use in elecrron microscopic observation and fixing the specimen thereon.
Claim 12
Independent Claim
79 words
A device for preparing a specimen, comprising a vessel containing a liquid containing a magnetic-labeled immunocomplex composed of a specimen and a magnetic-micro-particle obtainable by subjecting the specimen and the magnetic micro-particle bound thereto, a local concentration mechanism for locally concentrating the immunocomplex to a predetermined position, a recovery mechanism for recovering the specimen, and a guide mechanism for guiding the specimen onto the surface of a grid for use in elecrron microscopic observation and fixing the specimen thereon.
Claim 13
Independent Claim
110 words
A method for preparing a specimen, comprising a step of injecting a specimen to a magnetic-labeled body holding position at which a magnetic-labeled antibody composed of a magnetic micro-particle and an antibody or antigen bound to the magnetic micro-particle is held localized by means of external magnetic force, to subject the specimen and the magnetic-labeled body to antigen-antibody reaction, a step of recovering the magnetic-labeled body by releasing the application of the external magnetic force, wherein the holding of the magnetic-labeled body is performed by magnetizing a magnetic member inserted in the inside the reactor, and the recovery of the magnetic-labeled specimen is performed by demagnetization of the magnetic member.
Claim 14
Independent Claim
110 words
A method for preparing a specimen, comprising a step of injecting a specimen to a magnetic-labeled body holding position at which a magnetic-labeled antibody composed of a magnetic micro-particle and an antibody or antigen bound to the magnetic micro-particle is held localized by means of external magnetic force, to subject the specimen and the magnetic-labeled body to antigen-antibody reaction, a step of recovering the magnetic-labeled body by releasing the application of the external magnetic force, wherein the holding of the magnetic-labeled body is performed by magnetizing a magnetic member inserted in the inside the reactor, and the recovery of the magnetic-labeled specimen is performed by demagnetization of the magnetic member.
Claim 15
Dependent Claim
45 words
The method as claimed in Claim 9, wherein the magnetization of the magnetic member is performed in gradient magnetic field, and wherein the recovery of the magnetic-labeled body is performed by the demagnetization of the magnetic member in combination with vibration of th magnetic member.
Claim 16
Dependent Claim
35 words
The method as claimed in Claim 9, wherein the antigen-antibody reaction between the specimen and the magnetic-labeled body is performed with circulating the specimen through the magnetic-labeled body holding portion or vibrating the magnetic member.
Claim 17
Dependent Claim
35 words
The method as claimed in Claim 10, wherein the antigen-antibody reaction between the specimen and the magnetic-labeled body is performed with circulating the specimen through the magnetic-labeled body holding portion or vibrating the magnetic member.
Claim 18
Independent Claim
115 words
A device for preparing a specimen, comprising a reactor, a magnetic-labeled body holder provided with the reactor for holding a magnetic-labeled body composed of a specimen and a magnetic micro-particle bound to the specimen, a magnet provided with the magnetic labeled body holder and placed outside the reactor, a gradient magnetic field generating mechanism provided with the magnet for generating gradient magnetic field a magnetic member placed inside the reactor a specimen circulation mechanism for circulating the specimen, and a recovery mechanism for recovering the magnetic-labeled specimen, the recovery mechanism comprising a mechanism for giving relative movement to the magnet and the magnetic-labeled body holder to release the magnetic force applied to the magnetic-labeled specimen.
Claim 19
Independent Claim
115 words
A device for preparing a specimen, comprising a reactor, a magnetic-labeled body holder provided with the reactor for holding a magnetic-labeled body composed of a specimen and a magnetic micro-particle bound to the specimen, a magnet provided with the magnetic labeled body holder and placed outside the reactor, a gradient magnetic field generating mechanism provided with the magnet for generating gradient magnetic field a magnetic member placed inside the reactor a specimen circulation mechanism for circulating the specimen, and a recovery mechanism for recovering the magnetic-labeled specimen, the recovery mechanism comprising a mechanism for giving relative movement to the magnet and the magnetic-labeled body holder to release the magnetic force applied to the magnetic-labeled specimen.
Claim 20
Dependent Claim
84 words
14, A method for preparing a specimen, comprising a first step of magnetically labeling a specimen in a suspension containing the specimen a second step of performing local concentration of the specimen by applying gradient magnetic field to the suspension, wherein the specimen is concentrated to a predetermined position on or in the vicinity of the surface of the suspension, and a grid for use in electron microscopic observation is inserted to the position of local concentration to recover the specimen on the grid.
Claim 21
Dependent Claim
84 words
14, A method for preparing a specimen, comprising a first step of magnetically labeling a specimen in a suspension containing the specimen a second step of performing local concentration of the specimen by applying gradient magnetic field to the suspension, wherein the specimen is concentrated to a predetermined position on or in the vicinity of the surface of the suspension, and a grid for use in electron microscopic observation is inserted to the position of local concentration to recover the specimen on the grid.
Claim 22
Dependent Claim
58 words
15, The method as claimed in Claim 14, wherein gradient magnetic field is applied to the suspension from the side of the grid opposite to the side on which the magnetic-labeled specimen is recovered such that the magnetic field is the highest at the position of the grid to guide and fix the magnetic-labeled specimen to the grid.
Claim 23
Independent Claim
59 words
A device for preparing a specimen, comprising a mechanism for generating gradient magnetic field, a grid holder for holding a grid for use in electron microscopic observation at a position where gradient magnetic field is concentrated, and a guide mechanism for guiding the grid holder to a predetermined position of the surface of the suspension contained in a vessel.
Claim 24
Independent Claim
59 words
A device for preparing a specimen, comprising a mechanism for generating gradient magnetic field, a grid holder for holding a grid for use in electron microscopic observation at a position where gradient magnetic field is concentrated, and a guide mechanism for guiding the grid holder to a predetermined position of the surface of the suspension contained in a vessel.
References Cited (Backward Citations)
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