Patent CA998246A successfully refreshed from Google Patents.
EP0336731B1
Expired / Lapsed
EP
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Method of amplifying and detecting nucleic acid sequences
Assignee: City of Hope
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Filing: 1989-04-05
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Granted: 1994-05-11
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Expires: 2009-04-05
Abstract
No abstract record available.
Inventors
- 👤 Bruce R. Wallace
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 00:36
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/6862; A; A61; A61P; A61P7/00; C12Q1/6858
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
111 words
A method for amplifying a nucleic acid comprising a target sequence comprising the steps of: a) generating a single-stranded template comprising the target sequence; b) hybridising a pair of oligonucleotides to the template such that the oligonucleotides are juxtaposed for ligation on the template and each oligonucleotide is positioned to flank the target sequence; c) ligating the juxtaposed oligonucleotides to produce a double-stranded ligation product; d) denaturing the double stranded ligation product to produce an initial template strand and an oligonucleotide ligation product strand; and e) repeating steps (b) to (d) such that both the initial template strand and the oligonucleotide ligation strand act as templates for a further ligation reaction.
Claim 2
Independent Claim
111 words
A method for amplifying a nucleic acid comprising a target sequence comprising the steps of: a) generating a single-stranded template comprising the target sequence; b) hybridising a pair of oligonucleotides to the template such that the oligonucleotides are juxtaposed for ligation on the template and each oligonucleotide is positioned to flank the target sequence; c) ligating the juxtaposed oligonucleotides to produce a double-stranded ligation product; d) denaturing the double stranded ligation product to produce an initial template strand and an oligonucleotide ligation product strand; and e) repeating steps (b) to (d) such that both the initial template strand and the oligonucleotide ligation strand act as templates for a further ligation reaction.
Claim 3
Dependent Claim
23 words
A method according to claim 1 wherein the single stranded template is generated by disassociating the complementary strands of a double-stranded nucleic acid.
Claim 4
Dependent Claim
14 words
A method according to claim 2 wherein both complementary strands are used as templates.
Claim 5
Independent Claim
17 words
A method according to any one of claims 1 to 3 wherein the nucleic acid is DNA.
Claim 6
Independent Claim
14 words
A method according to claims 1 to 3 wherein the nucleic acid is RNA.
Claim 7
Independent Claim
15 words
A method according to any preceding claim wherein the target sequence is a single nucleotide.
Claim 8
Independent Claim
42 words
A method according to any preceding claim further comprising producing an amplification product by repetition of step (e) and subsequently determining the presence or absence of a nucleic acid sequence complementary to or identical to the target sequence in the amplification product.
Claim 9
Independent Claim
27 words
A method for the diagnosis of a genetic disease comprising analysing the genotype of a patient by the method of any one of claims 1 to 7.
Claim 10
Dependent Claim
13 words
A method according to claim 8 wherein the disease is sickle cell anaemia.
References Cited (Backward Citations)
No citation records indexed.
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