Patent EP0336412A2 successfully refreshed from Google Patents.
EP0336412A2
Expired / Lapsed
EP
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Synthetic oligonucleotides useful for the determination of Chlamydia trachomatis in a biological sample
Assignee: Sclavo SpA
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Filing: 1989-04-06
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Granted: —
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Expires: 2009-04-06
Abstract
Abstract The nucleotide sequence of the PCHL1 plasmid which is specific to Chlamydia trachomatis (CT) is reported. Synthetic oligonucleotides with sequences homologous or corresponding to a region of at least 10 consecutive nucleotide bases selected from the sequence of the plasmid are particularly useful for the determination of CT in a biological sample.
Inventors
- 👤 Giulio Ratti
- 👤 Maurizio Comanducci
- 👤 Stefano Ricci
- 👤 Giancarlo Garuti
- 👤 Egidio Cosco
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 09:05
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/295; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6841; C12Q1/6876; C12Q1/6888; C12Q1/689
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
45 words
Synthetic oligonucleotides useful for the determination of Chlamydia trachomatis in a biological sample characterised by a nucleotide sequence homologous to a region of at least 10 consecutive nucleotide bases selected from the sequence of the plasmid specific to Chlamydia trachomatis , given in Figure 1.
Claim 2
Dependent Claim
45 words
Synthetic oligonucleotides useful for the determination of Chlamydia trachomatis in a biological sample characterised by a nucleotide sequence homologous to a region of at least 10 consecutive nucleotide bases selected from the sequence of the plasmid specific to Chlamydia trachomatis , given in Figure 1.
Claim 3
Dependent Claim
19 words
Synthetic oligonucleotides according to claim 1, in which the number of consecutive nucleotide bases is between 20 and 100.
Claim 4
Dependent Claim
15 words
Synthetic oligonucleotides according to claim 1, in which the nucleotide sequence does not contain palindromes.
Claim 5
Dependent Claim
31 words
Synthetic oligonucleotides according to claim 1, in which the nucleotide sequence is homologous to at least one repeated region contained in the sequence of the plasmid specific to Chlamydia trachomatis .
Claim 6
Dependent Claim
34 words
Synthetic oligonucleotides according to claim 4, in which the region is contained in the fragment of approximately 200 base pairs between positions 6426 and 6611 of the sequence of the plasmid Chlamydia trachomatis .
Claim 7
Independent Claim
89 words
A method for the determination of Chlamydia trachomatis in a clinical sample, which comprises the denaturing of the double-stranded nucleic acids extracted from the disrupted cells contained in the sample under test, the placing of the single-stranded nucleic acids thus obtained in contact with one or more probes having nucleotide sequences homologous to at least one sequence contained in the strands and the detection of the hybridised probe, the method being characterised in that the probes are selected from the group of oligonucleotides according to claims 1 to 5.
Claim 8
Dependent Claim
43 words
A method according to claim 6, in which the single-stranded nucleic acids are ligated to an insoluble solid substrate and then placed in contact, in a hybridisation mixture, with one or more of the oligonucleotides according to claims 1 to 5, suitably marked.
Claim 9
Dependent Claim
19 words
A method according to claim 7, in which the marker substances are selected from the group of radioactive isotopes.
Claim 10
Dependent Claim
26 words
A method according to claim 7, in which the marker substances are selected from the group of haptens, antigens, antibodies, biotin, fluorescent substances and chemiluminescent substances.
Claim 11
Dependent Claim
29 words
A method according to claim 6, characterised in that the determination of Chlamydia trachomatis is carried out by means of the hybridisation in situ of the samples under test.
Claim 12
Dependent Claim
71 words
A method according to claim 6, in which a fragment of the single-stranded DNA of the plasmid Chlamydia trachomatis which may be present in the sample under test is amplified in vitro by means of the use of synthetic oligonucleotides according to claim 1, where the oligonucleotides are characterised by sequences capable of ligating to the fragment of plasmid DNA to be amplified at a predetermined distance and on opposite strands.
Claim 13
Dependent Claim
23 words
A method according to claim 11, in which the amplified plasmid DNA fragment contains the presumed origin of replication of the pCHL1 plasmid.
Claim 14
Dependent Claim
24 words
A method according to claim 12, in which the plasmid DNA fragment is constituted by 0.186 Kilobases and the oligonucleotides are P1 and P2.
Claim 15
Dependent Claim
17 words
A method according to claim 11, in which the amplified plasmid DNA fragment is displayed by electrophoresis.
Claim 16
Dependent Claim
33 words
A method according to claim 11, in which the amplified plasmid DNA fragment is displayed by hybridisation with a marked oligonucleotide having a sequence complementary to a sequence contained in the amplified fragment.
Claim 17
Dependent Claim
12 words
A method according to claim 15, in which the oligonucleotide is P3.
Claim 18
Dependent Claim
19 words
A plasmid specific to Chlamydia trachomatis characterised by the nucleotide sequence of 7499 base pairs given in Figure 1.
Claim 19
Dependent Claim
19 words
A plasmid specific to Chlamydia trachomatis characterised by the nucleotide sequence of 7499 base pairs given in Figure 1.
Claim 20
Dependent Claim
28 words
A diagnostic kit for the determination of Chlamydia trachomatis in a biological sample, characterised in that it contains one or more oligonucleotides according to claims 1 to 5.
Claim 21
Dependent Claim
13 words
A diagnostic kit according to claim 18, in which the oligonucleotides are marked.
Claim 22
Dependent Claim
53 words
A diagnostic kit according to claim 18, which includes two oligonucleotides useful for the amplification of a fragment contained in the DNA sequence of the plasmid of Chlamydia trachomatis which may be present in the sample under test and a marked oligonucleotide which is complementary to a sequence contained in the amplified fragment.
Claim 23
Dependent Claim
25 words
A diagnostic kit according to claim 20, in which the oligonucleotides useful for the amplification are P1 and P2 and the marked oligonucleotide is P3.
References Cited (Backward Citations)
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