Patent US2988052A successfully refreshed from Google Patents.
Patent EP0330221A2 successfully refreshed from Google Patents.
EP0330221A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
End labeled nuceotide probe
Assignee: Enzo Biochem Inc
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Filing: 1989-02-24
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Granted: —
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Expires: 2009-02-24
Abstract
Abstract An oligo- or polynucleotide having at least one biotin directly or indirectly attached to each of the 5' and 3' end nucleotides thereof and a nucleic acid hybridization assay system for use therewith. The biotins are optionally attached through linkage groups that do not interfer with hybridization. The biotins can further serve to attach a biotinylated polymer.
Inventors
- 👤 Christine L. Brakel
- 👤 Alan F. Cook
- 👤 Edmund Vuocolo
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 20:58
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6832; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
24 words
) An oligo- or polynucleotide having at least one biotin directly or indirectly attached to each of the 5 and 3 end nucleotides thereof.
Claim 2
Dependent Claim
24 words
) An oligo- or polynucleotide having at least one biotin directly or indirectly attached to each of the 5 and 3 end nucleotides thereof.
Claim 3
Dependent Claim
20 words
) The oligo- or polynucleotide of claim 1 comprising at least 2-5 biotins attached to at least one end thereof.
Claim 4
Dependent Claim
15 words
) The oligo- or polynucleotide of claim 2 comprising 2-5 biotins at each end thereof.
Claim 5
Dependent Claim
26 words
) The oligo- or polynucleotide of claim 1 wherein at least one of said biotins is attached to the terminal nucleotide through a non-interfering linkage group.
Claim 6
Dependent Claim
14 words
) The oligo- or polynucleotide of claim 4 wherein said biotin attachment comprises biotin-11-dUMP
Claim 7
Dependent Claim
22 words
) The oligo- or polynucleotide of claim 1 wherein at least one of said end nucleotides is attached to a polybiotinylated polymer.
Claim 8
Dependent Claim
15 words
) The oligo- or polynucleotide of claim 6 wherein said polybiotinylated polymer is polybiotinylated dextran.
Claim 9
Dependent Claim
33 words
8) An oligo- or polynucleotide having at least one biotin or modifiable group attached to each of the 5' and 3 terminal nucleotides external to target hybridization regions of said oligo- or polynucleotide.
Claim 10
Dependent Claim
33 words
8) An oligo- or polynucleotide having at least one biotin or modifiable group attached to each of the 5' and 3 terminal nucleotides external to target hybridization regions of said oligo- or polynucleotide.
Claim 11
Dependent Claim
26 words
9) A nucleic acid hybridization assay composition comprising an oligo- or polynucleotide of any of claims 1-8 and a preformed avidin or streptavidin detectable molecule complex.
Claim 12
Dependent Claim
16 words
10) The composition of claim 9 wherein the detectable molecule is a fluor, chromogen or enzyme.
Claim 13
Dependent Claim
19 words
11) The composition of claim 10 wherein the enzyme is selected from peroxidase, alkaline phosphatase, acid phosphatase and beta-galactosidase.
References Cited (Backward Citations)
No citation records indexed.
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