Patent US6180613B1 successfully refreshed from Google Patents.
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EP0312394A2
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Membrane-supported immunoassays
Assignee: Novelion Therapeutics Inc
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Filing: 1988-10-14
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Granted: —
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Expires: 2008-10-14
Abstract
Abstract Devices for immunoassays employing a reusable syringe or vacuum manifold to pass samples, by means of a pressure gradient through a membrane containing an affinity partner for analyte. The devices can also be adapted to direct blood sampling and to automated assays.
Inventors
- 👤 Howard Chandler
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 14:38
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/5436; G01N33/54366; G01N33/74; G01N33/76; G01N35/00; G01N35/10; G01N2035/1027; G01N2035/1048; G01N2035/1055; Y; Y10; Y10S; Y10S436/00; Y10S436/807
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
62 words
A method to conduct an assay for an analyte in a sample, wherein the analyte or derivative thereof binds to an affinity partner, which method comprises: passing a volume of said sample through a membrane by means of a pressure gradient wherein the membrane contains the affinity partner for the analyte or derivative thereof, and detecting the analyte bound in the membrane.
Claim 2
Independent Claim
62 words
A method to conduct an assay for an analyte in a sample, wherein the analyte or derivative thereof binds to an affinity partner, which method comprises: passing a volume of said sample through a membrane by means of a pressure gradient wherein the membrane contains the affinity partner for the analyte or derivative thereof, and detecting the analyte bound in the membrane.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the sample is passed through the membrane by withdrawing said sample into a receiving chamber.
Claim 4
Dependent Claim
19 words
The method of claim 2 wherein the receiving chamber is the body of a syringe or a vacuum manifold.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein the sample is passed through the membrane by applying pressure to the sample against the membrane.
Claim 6
Dependent Claim
21 words
The method of claim 1 wherein the affinity partner for the analyte or its derivative is an immunoglobulin or derivative thereof.
Claim 7
Independent Claim
74 words
A method to conduct an assay for analyte in two or more samples simultaneously wherein the analyte binds to an affinity partner, which method comprises: placing a volume of each sample in contact with a membrane in which the affinity partner for the analyte is contained, and withdrawing each sample by means of a pressure gradient through the membrane into a receiving chamber, and detecting the analyte found in each membrane from each sample.
Claim 8
Independent Claim
74 words
A method to conduct an assay for analyte in two or more samples simultaneously wherein the analyte binds to an affinity partner, which method comprises: placing a volume of each sample in contact with a membrane in which the affinity partner for the analyte is contained, and withdrawing each sample by means of a pressure gradient through the membrane into a receiving chamber, and detecting the analyte found in each membrane from each sample.
Claim 9
Dependent Claim
31 words
The method of claim 6 wherein each sample is placed in an outer chamber affixed to the corresponding membrane and withdrawn by means of a pressure gradient into a vacuum manifold.
Claim 10
Independent Claim
48 words
An apparatus for the detection of analyte in a sample wherein the detection comprises reaction with an affinity partner, which apparatus comprises: a supported membrane containing said affinity partner, said membrane supported in a housing having means to attach said membrane to the orifice of a receiving chamber.
Claim 11
Independent Claim
48 words
An apparatus for the detection of analyte in a sample wherein the detection comprises reaction with an affinity partner, which apparatus comprises: a supported membrane containing said affinity partner, said membrane supported in a housing having means to attach said membrane to the orifice of a receiving chamber.
Claim 12
Dependent Claim
17 words
The apparatus of claim 8 which further includes the body of a syringe attached to the housing.
Claim 13
Dependent Claim
17 words
The apparatus of claim 8 wherein said housing engages said membrane by means of a deformable lip.
Claim 14
Dependent Claim
23 words
The apparatus of claim 8 which further includes an outer chamber disposed at the top of the housing capable of accommodating the sample.
Claim 15
Independent Claim
86 words
A method to conduct an assay for analyte in a sample, wherein the analyte binds to an affinity partner, and wherein said binding is detected by reaction with a labeled second affinity partner, wherein one of said first and second affinity partners is specific for analyte, which method comprises: passing a volume of said sample, by means of a pressure gradient, through a porous support containing said labelled second affinity partner and through a membrane in which the first affinity partner for the analyte is contained.
Claim 16
Independent Claim
86 words
A method to conduct an assay for analyte in a sample, wherein the analyte binds to an affinity partner, and wherein said binding is detected by reaction with a labeled second affinity partner, wherein one of said first and second affinity partners is specific for analyte, which method comprises: passing a volume of said sample, by means of a pressure gradient, through a porous support containing said labelled second affinity partner and through a membrane in which the first affinity partner for the analyte is contained.
Claim 17
Independent Claim
59 words
An apparatus for conducting an assay for analyte in a sample which comprises: a needle; attached to a housing in which is supported a membrane containing an affinity partner for the analyte, which membrane is connected to a receiving chamber which includes a means of applying a pressure gradient through the needle and past the membrane into the chamber.
Claim 18
Independent Claim
59 words
An apparatus for conducting an assay for analyte in a sample which comprises: a needle; attached to a housing in which is supported a membrane containing an affinity partner for the analyte, which membrane is connected to a receiving chamber which includes a means of applying a pressure gradient through the needle and past the membrane into the chamber.
Claim 19
Independent Claim
71 words
An apparatus for conducting an assay for an analyte in a sample which comprises a means for applying a pressure gradient past a membrane in which a first affinity partner for the analyte is contained, into a receiving chamber and having upstream of said membrane a porous support containing a second affinity partner conjugated to label, wherein at least one of said first and second affinity partners is specific for analyte.
Claim 20
Independent Claim
71 words
An apparatus for conducting an assay for an analyte in a sample which comprises a means for applying a pressure gradient past a membrane in which a first affinity partner for the analyte is contained, into a receiving chamber and having upstream of said membrane a porous support containing a second affinity partner conjugated to label, wherein at least one of said first and second affinity partners is specific for analyte.
Claim 21
Dependent Claim
35 words
A kit suitable for conducting measurement of an analyte in a biological sample which reagent kit comprises: the supported membrane unit of claim 8, appropriate reagents in separate bottles, and instructions for conducting the assay.
References Cited (Backward Citations)
No citation records indexed.
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