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EP0306610A1 Expired / Lapsed EP ⏱️ 0.0 years remaining

Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase

Assignee: GD Searle LLC
•
Filing: 1983-03-17
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Granted: —
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Expires: 2003-03-17
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Abstract

Abstract A method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminents by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.

Inventors

  • 👤 Stephen James Brewer
  • 👤 Helmut Max Sassenfeld

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-08 02:21
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/02; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/485; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/50; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S930/00; Y10S930/01; Y10S930/12

Claims Analysis

Total Claims: 4
Claim 1
Independent Claim 52 words
A method of quantitating the presence of a polypeptide with a covalently attached polymer of charged amino acids comprising: a) hydrolyzing the covelently attached polymer of charged amino acids with an exopeptidase; b) quantitating the number of amino acids released by the exopeptidase hydrolysis as a measure of the said polypeptide present.
Claim 2
Independent Claim 52 words
A method of quantitating the presence of a polypeptide with a covalently attached polymer of charged amino acids comprising: a) hydrolyzing the covelently attached polymer of charged amino acids with an exopeptidase; b) quantitating the number of amino acids released by the exopeptidase hydrolysis as a measure of the said polypeptide present.
Claim 3
Independent Claim 45 words
A method of quantitating the presence of a polypeptide with a covalent polymer of charged amino acids comprising: a) binding the covalently attached polymer of charged amino acids with a detectable ligand, b) quantitating the detectable ligand as a measure of the said polypeptide present.
Claim 4
Independent Claim 45 words
A method of quantitating the presence of a polypeptide with a covalent polymer of charged amino acids comprising: a) binding the covalently attached polymer of charged amino acids with a detectable ligand, b) quantitating the detectable ligand as a measure of the said polypeptide present.

References Cited (Backward Citations)

EP0001930A2 Cited
EP0020290A1 Cited
EP0035384A2 Cited

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