Patent EP0303309A2 successfully refreshed from Google Patents.
EP0303309A2
Expired / Lapsed
EP
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A genus-specific Listeria antigen identified by monoclonal antibodies
Assignee: Akzo Nobel NV
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Filing: 1988-07-15
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Granted: —
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Expires: 2008-07-15
Abstract
Abstract Antigens characteristic of all species of Listeria except L. denitrificans comprising proteins found in Listeria heat extracts, the major antigen having a molecular weight of from about 30 to about 38 kD and comprising three immunogenically different epitopes and others comprising proteins having a molecular weight range of approximately 17 kD to the major antigen and comprising an epitope immunoreactive with antibodies that are also reactive with one of the three epitopes on the 30 to about 38 kD protein. The invention also comprises mouse monoclonal antibodies specifically reactive with the identified epitopes on these antigens.
Inventors
- 👤 Bryan Timothy Butman
- 👤 Jerome Arnold Mattingly
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 11:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C07; C07K; C07K16/00; C07K16/12; C07K16/1267; C07K16/1296; C07K14/00; C07K14/195; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S530/00; Y10S530/806; Y10S530/808; Y10S530/809; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
30 words
Antigen characteristic for Listeria species with the exception of L. denitrificans reactive with at least one of the monoclonal antibodies selected from the group consisting of 9-5E, 10-12C and 10-2A.
Claim 2
Dependent Claim
30 words
Antigen characteristic for Listeria species with the exception of L. denitrificans reactive with at least one of the monoclonal antibodies selected from the group consisting of 9-5E, 10-12C and 10-2A.
Claim 3
Dependent Claim
66 words
Antigen according to claim 1 comprising a protein found in Listeria heat extracts having a molecular weight of from about 30 to about 38 kD, the antigen comprising three immunogenically different epitopes, to which antibodies produced by spleen cells of mice immunized with said Listeria heat extracts are specifically reactive, said antigen being found in heat extracts of all species of Listeria except L. denitrificans .
Claim 4
Dependent Claim
42 words
A Listeria antigen of claim 2, wherein a Group I IgG₁ antibody is reactive with the first epitope, a Group II IgG₁ antibody is reactive with a second epitope, and a Group III IgG 2a antibody is reactive with a third epitope.
Claim 5
Dependent Claim
51 words
4 A Listeria antigen comprising a protein found in Listeria heat extracts having a molecular weight less than the major antigen but at least 17 kD, said antigen comprising an epitope to which a monoclonal antibody is immunoreactive that is also immunoreactive with an epitope on the antigen of claim 1.
Claim 6
Dependent Claim
10 words
5 Antibody immunoreactive with the Listeria antigen of claim 1.
Claim 7
Dependent Claim
28 words
Monoclonal antibody against the antigen according to claim 1 characterized by an epitope specificity of a monoclonal antibody selected from the group consisting of 9-5E, 10-12C and 10-2A.
Claim 8
Dependent Claim
41 words
7 The monoclonal antibody of claim 6, produced by immunizing an animal with Listeria heat extracts, immortalizing antibody producing cells from said animal, culturing the immortalized cells to produce antibodies, and selecting immortalized cells that produce antibodies immunoreactive with the antigen.
Claim 9
Dependent Claim
32 words
Immuno-assay method for the detection of Listeria in a sample characterized in that said sample is incubated with at least one antibody which is immunoreactive with the antigen according to claim 1.
Claim 10
Dependent Claim
20 words
9 Immuno-assay test kit for detection of Listeria comprising at least one antibody immunoreactive with the antigen according to claim.
Claim 11
Dependent Claim
26 words
10 Immuno-reagent comprising an antigen according to claim 1 or an antibody immuno-reactive with said antigen bound to a solid carrier, a label or a particle.
References Cited (Backward Citations)
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