Patent EP0021571A1 successfully refreshed from Google Patents.
Patent EP0302715A1 successfully refreshed from Google Patents.
EP0302715A1
Expired / Lapsed
EP
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Avidin- and biotin-immobilized reagents, analytical elements and methods of use
Assignee: Eastman Kodak Co
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Filing: 1988-08-03
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Granted: —
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Expires: 2008-08-03
Abstract
Abstract Reagents have been prepared from water insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.
Inventors
- 👤 Richard Calvin C/O Eastman Kodak Co. Sutton
- 👤 Susan Jean C/O Eastman Kodak Co. Danielson
- 👤 Brent Arthur C/O Eastman Kodak Co. Burdick
- 👤 Harold Chester Iii C/O Eastman Kodak Co. Warren
- 👤 Brian Anthony C/O Eastman Kodak Co. Snyder
- 👤 Gregory Joseph c/o Eastman Kodak Co. McClune
- 👤 Annie Liu C/O Eastman Kodak Co. Wu
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 19:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54306; G01N33/544; G01N33/545
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
77 words
A water-insoluble reagent comprising: a polymeric particle comprising a polymer on at least its outer surface which is derived from one or more ethylenically unsaturated polymerizable monomers, the reagent characterized wherein at least one of the monomers has reactive groups which are activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom groups, and the particle is covalently attached through the reactive groups on the outer surface of the particle to avidin, biotin or an avidin or biotin derivative.
Claim 2
Dependent Claim
77 words
A water-insoluble reagent comprising: a polymeric particle comprising a polymer on at least its outer surface which is derived from one or more ethylenically unsaturated polymerizable monomers, the reagent characterized wherein at least one of the monomers has reactive groups which are activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom groups, and the particle is covalently attached through the reactive groups on the outer surface of the particle to avidin, biotin or an avidin or biotin derivative.
Claim 3
Dependent Claim
14 words
The reagent as claimed in claim 1 having a detectable tracer material associated therewith.
Claim 4
Dependent Claim
20 words
The reagent as claimed in either claim 1 or 2 having avidin or a derivative thereof attached to the particle.
Claim 5
Dependent Claim
109 words
The reagent as claimed in any one of claims 1 to 3 wherein the polymer is represented by the formula: wherein -A- represents recurring units derived from one or more hydrophobic ethylenically unsaturated polymerizable monomers, -B- represents recurring units derived from one or more ethylenically unsaturated polymerizable monomers having a reactive group which is an activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom, -D- represents recurring units derived from one or more ethylenically unsaturated polymerizable monomers other than those represented by -A- or -B-, x is from 0 to 99.9 mole percent, y is from 0.1 to 100 mole percent, and z is from 0 to 20 mole percent.
Claim 6
Dependent Claim
117 words
The reagent as claimed in any one of claims 1 to 4 wherein the activated 2-substituted ethylsulfonyl and vinylsulfonyl monomers are represented by the formula: wherein R is hydrogen or substituted or unsubstituted alkyl, R¹ is -CH=CHR² or -CH₂CH₂X wherein X is a leaving group which is displaced by a nucleophile or is eliminated in the form of HX by treatment with a base, and R² is hydrogen, substituted or unsubstituted alkyl, or substituted or unsubstituted aryl, and L is a substituted or unsubstituted alkylene, substituted or unsubstituted arylene, a combination of one or more of each of the alkylene and arylene groups, or such combination interrupted or terminated with one or more amide or ester groups.
Claim 7
Dependent Claim
26 words
The reagent as claimed in claim 5 wherein R is hydrogen or methyl, R¹ is -CH₂CH₂X and L is substituted or unsubstituted phenylenealkylene, carbonyliminoarylenealkylene, or carbonyliminoalkyleneiminocarbonylalkylene.
Claim 8
Dependent Claim
38 words
An element comprising an absorbent carrier material having one or more zones, the element characterized wherein it contains in one or more of the zones a water-insoluble reagent as claimed in any one of claims 1 to 6.
Claim 9
Independent Claim
64 words
A method for the determination of a compound of biological interest in an aqueous liquid comprising: A. contacting the liquid with a water-insoluble reagent as claimed in any one of claims 1 to 6, B. forming a reaction product of the reagent with the biological compound, and C. determining the amount of biological compound as a result of the presence of the reaction product.
Claim 10
Dependent Claim
43 words
The method as claimed in claim 8 for the determination of hCG wherein the immunoreactive species of the reagent is a first antibody against hCG, and wherein the method is carried out with a second antibody against hCG which is labeled for detection.
Claim 11
Dependent Claim
16 words
The method as claimed in claim 9 wherein the second antibody is labeled with an enzyme.
Claim 12
Dependent Claim
14 words
The method as claimed in claim 8 for the determination of a human retrovirus.
Claim 13
Independent Claim
263 words
A method for the determination of a compound of biological interest in an aqueous liquid comprising: A. contacting the liquid with a water-insoluble reagent comprising: a core/shell polymeric particle in which the shell comprises a polymer which is represented by the formula: wherein -A- represents recurring units derived from one or more hydrophobic ethylenically unsaturated monomers, -B- represents recurring units derived from one or more ethylenically unsaturated monomers having a reactive group which is an activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom, -D- represents recurring units derived from one or more ethylenically unsaturated monomers other than those represented by -A- or -B-, x is from 0 to 99.9 mole percent, y is from 0.1 to 100 mole percent, and z is from 0 to 20 mole percent, the particle being covalently attached through the reactive groups on the outer surface of the shell to avidin or a derivative thereof, B. forming a reaction product of the reagent with a biotinylated reagent comprised of biotin or a derivative thereof attached to a first receptor molecule for the biological compound so as to form an insoluble specific binding complex between avidin and the biotinylated reagent, C. prior to, simultaneously with or subsequent to contacting step A, contacting the biological compound with a second receptor molecule which is capable of participating in a specific binding reaction with the biological compound but which is not reactive with the first receptor molecule, the second receptor being labeled with a detectable tracer material, so as to form a labeled insoluble complex, and D. detecting the labeled insoluble complex.
Claim 14
Independent Claim
263 words
A method for the determination of a compound of biological interest in an aqueous liquid comprising: A. contacting the liquid with a water-insoluble reagent comprising: a core/shell polymeric particle in which the shell comprises a polymer which is represented by the formula: wherein -A- represents recurring units derived from one or more hydrophobic ethylenically unsaturated monomers, -B- represents recurring units derived from one or more ethylenically unsaturated monomers having a reactive group which is an activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom, -D- represents recurring units derived from one or more ethylenically unsaturated monomers other than those represented by -A- or -B-, x is from 0 to 99.9 mole percent, y is from 0.1 to 100 mole percent, and z is from 0 to 20 mole percent, the particle being covalently attached through the reactive groups on the outer surface of the shell to avidin or a derivative thereof, B. forming a reaction product of the reagent with a biotinylated reagent comprised of biotin or a derivative thereof attached to a first receptor molecule for the biological compound so as to form an insoluble specific binding complex between avidin and the biotinylated reagent, C. prior to, simultaneously with or subsequent to contacting step A, contacting the biological compound with a second receptor molecule which is capable of participating in a specific binding reaction with the biological compound but which is not reactive with the first receptor molecule, the second receptor being labeled with a detectable tracer material, so as to form a labeled insoluble complex, and D. detecting the labeled insoluble complex.
Claim 15
Dependent Claim
26 words
The method as claimed in claim 12 wherein the biological compound is an antigen and the first and second receptors are distinct antibodies to the antigen.
References Cited (Backward Citations)
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