Patent EP0295808A2 successfully refreshed from Google Patents.
EP0295808A2
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EP
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Liquid chromatography packing materials and method for making and using same
Assignee: Dow Silicones Corp
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Filing: 1988-06-01
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Granted: —
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Expires: 2008-06-01
Abstract
Abstract Packing materials for use in liquid chromatographic analysis are prepared by contacting a porous support, such as porous silica, with a ketal silane to form ketal blocked diol groups on the surfaces thereof. An end-capping reagent such as a trimethylsilyl reagent may be added. In addition, the ketal blocked diol groups may be further hydrolyzed to diol groups.
Inventors
- 👤 Dwight Edward Williams
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 04:41
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/287; B01J20/30; B01J20/32; B01J20/3214; B01J20/3225; B01J20/3227; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3263; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/32; B01D15/325; B01D15/34; B01J2220/00; B01J2220/50; B01J2220/54; G; G01; G01N; G01N30/00; G01N30/02
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
23 words
A reverse phase packing material for liquid chromatographic columns which comprises a porous support having ketal blocked diol groups on the surfaces thereof.
Claim 2
Dependent Claim
23 words
A reverse phase packing material for liquid chromatographic columns which comprises a porous support having ketal blocked diol groups on the surfaces thereof.
Claim 3
Dependent Claim
13 words
The packing material of claim 1 wherein said porous support is porous silica.
Claim 4
Dependent Claim
15 words
The packing material of claim 2 wherein said ketal blocked diol groups have the formula
Claim 5
Dependent Claim
20 words
The packing material of claim 3 wherein said porous silica support also has trimethylsilyl end-capping groups on the surfaces thereof.
Claim 6
Dependent Claim
19 words
The packing material of claim 3 wherein said porous silica support also has octadecylsilyl groups on the surfaces thereof.
Claim 7
Dependent Claim
26 words
A size exclusion chromatography packing material for liquid chromatographic columns which comprises a porous support having on the surfaces thereof diol groups of the following formula
Claim 8
Dependent Claim
26 words
A size exclusion chromatography packing material for liquid chromatographic columns which comprises a porous support having on the surfaces thereof diol groups of the following formula
Claim 9
Dependent Claim
13 words
The packing material of claim 6 wherein said porous support is porous silica.
Claim 10
Dependent Claim
20 words
The packing material of claim 7 wherein said porous silica support also has trimethylsilyl end-capping groups on the surfaces thereof.
Claim 11
Independent Claim
86 words
A method for making a packing material for liquid chromatographic columns which comprises: a) providing a hydroxyl-bearing porous support selected from the group consisting of porous metalloid oxides, porous metallic oxides and porous mixed metallic oxides, and b) contacting said porous support with a ketal silane having the formula: wherein L is chloro, NR₂, or N-methylacetamido, Me is methyl, and m is 1-3, in sufficient amounts and for a sufficient period of time to form ketal blocked diol groups on the surfaces of said porous support.
Claim 12
Independent Claim
86 words
A method for making a packing material for liquid chromatographic columns which comprises: a) providing a hydroxyl-bearing porous support selected from the group consisting of porous metalloid oxides, porous metallic oxides and porous mixed metallic oxides, and b) contacting said porous support with a ketal silane having the formula: wherein L is chloro, NR₂, or N-methylacetamido, Me is methyl, and m is 1-3, in sufficient amounts and for a sufficient period of time to form ketal blocked diol groups on the surfaces of said porous support.
Claim 13
Dependent Claim
15 words
The method of claim 9 wherein said porous support is porous silica containing silanol groups.
Claim 14
Dependent Claim
19 words
The method of claim 10 further including the step of hydrolyzing said ketal blocked diol groups to diol groups.
Claim 15
Dependent Claim
33 words
The method of claim 10 further including the step of contacting said porous silica with an excess of a trimethylsilyl end-capping reagent to cap remaining silanols on the surfaces of said porous silica.
Claim 16
Dependent Claim
11 words
The method of claim 12 wherein said end-capping reagent is ((CH₃)₃Si)₂NH.
Claim 17
Dependent Claim
9 words
The method of claim 9 wherein said ketal silane
Claim 18
Independent Claim
54 words
A method for analysis by reverse phase liquid chromatography, comprising: (a) preparing an analyte containing blood serum, blood plasma, or other biological fluid to be analyzed, and (b) contacting said analyte with a liquid chromatography column packed with a packing material comprising a porous support having ketal blocked diol groups on the surfaces thereof.
Claim 19
Independent Claim
54 words
A method for analysis by reverse phase liquid chromatography, comprising: (a) preparing an analyte containing blood serum, blood plasma, or other biological fluid to be analyzed, and (b) contacting said analyte with a liquid chromatography column packed with a packing material comprising a porous support having ketal blocked diol groups on the surfaces thereof.
Claim 20
Dependent Claim
12 words
The method of claim 15 wherein said porous support is porous silica.
Claim 21
Dependent Claim
14 words
The method of claim 16 wherein said ketal blocked diol groups have the formula
Claim 22
Dependent Claim
19 words
The method of claim 17 wherein said porous silica support also has trimethylsilyl end-capping groups on the surfaces thereof.
Claim 23
Independent Claim
45 words
A method for analysis by size exclusion chromatography, comprising: (a) preparing an analyte for analysis, and (b) contacting said analyte with a liquid chromatography column packed with a packing material comprising a porous support having on the surfaces thereof diol groups of the following formula:
Claim 24
Independent Claim
45 words
A method for analysis by size exclusion chromatography, comprising: (a) preparing an analyte for analysis, and (b) contacting said analyte with a liquid chromatography column packed with a packing material comprising a porous support having on the surfaces thereof diol groups of the following formula:
Claim 25
Dependent Claim
12 words
The method of claim 19 wherein said porous support is porous silica.
Claim 26
Dependent Claim
19 words
The method of claim 20 wherein said porous silica support also has trimethylsilyl end-capping groups on the surfaces thereof.
References Cited (Backward Citations)
No citation records indexed.
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