Patent EP0293274A1 successfully refreshed from Google Patents.
EP0293274A1
Expired / Lapsed
EP
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DNA molecules encoding non-A, non-B hepatitis antigens, and their use in producing said antigens
Assignee: Mitsubishi Chemical Corp
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Filing: 1988-03-31
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Granted: —
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Expires: 2008-03-31
Abstract
Abstract Provided herein are a DNA fragment which contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein occurring in cells of the liver affected with non-A non-B hepatitis, an expression vector in which said DNA fragment is inserted into a cloning site present downstream from a promoter thereof, a transformant obtained by introducing said expression vector into a host, and a process for producing said antigenic protein which comprises providing said expression vector, transforming a host with said expression vector, culturing the transformed host and collecing the protein produced therein.
Inventors
- 👤 Kazuhiro 303 Corpo Omodaka Takahashi
- 👤 Tatsurou Shibui
- 👤 Michiru Kamizono
- 👤 Rie Matsui
- 👤 Yutaka Teranishi
- 👤 Shigetada Nakanishi
- 👤 Naomi Kitamura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 08:14
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/005; C12N2770/00; C12N2770/00011; C12N2770/24011; C12N2770/24211; C12N2770/24222
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
27 words
A DNA fragment which contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein occurring in cells of the liver affected with non-A non-B hepatitis.
Claim 2
Dependent Claim
27 words
A DNA fragment which contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein occurring in cells of the liver affected with non-A non-B hepatitis.
Claim 3
Dependent Claim
23 words
The DNA fragment in accordance with claim 1, in which the cells of the liver are derived from a human or chimpanzee individual.
Claim 4
Dependent Claim
33 words
The DNA fragment in accordance with claim 1, in which the antigenic protein specific to non-A non-B hepatitis has the whole or a part of the amino acid sequence represented by the formula:
Claim 5
Dependent Claim
43 words
The DNA fragment in accordance with claim 1, in which the base sequence comprises the whole or a part of the base sequence represented by the formula: wherein the sign "-" represents a base complementary to the base shwon just above each sign.
Claim 6
Dependent Claim
33 words
An expression vector in which a DNA fragment containing a base sequence coding for a non-A non-B hepatitis-specific antigen is intoduced into a cloning site present downstream from a promoter of said vector.
Claim 7
Dependent Claim
33 words
An expression vector in which a DNA fragment containing a base sequence coding for a non-A non-B hepatitis-specific antigen is intoduced into a cloning site present downstream from a promoter of said vector.
Claim 8
Dependent Claim
18 words
The expression vector in accordance with claim 5, in which the promoter is controllable by a regulatory factor.
Claim 9
Dependent Claim
16 words
The expression vector in accordance with claim 5, in which the promoter operates in a microorganism.
Claim 10
Dependent Claim
16 words
The expression vector in accordance with claim 5, in which the promoter operates in an eukaryote.
Claim 11
Dependent Claim
41 words
A transformant obtained by transforming a host with an expression vector in which a DNA fragment containing a base sequence coding for a non-A non-B hepatitis-specific antigen is introduced into a cloning site present downstream from a promoter of said vector.
Claim 12
Dependent Claim
41 words
A transformant obtained by transforming a host with an expression vector in which a DNA fragment containing a base sequence coding for a non-A non-B hepatitis-specific antigen is introduced into a cloning site present downstream from a promoter of said vector.
Claim 13
Dependent Claim
17 words
The transformant in accordance with claim 9, in which the host is Escherichia coli or Bacillus subtilis.
Claim 14
Dependent Claim
61 words
A process for producing an antigen specific to non-A non-B hepatitis comprising introducing a DNA fragment containing a base sequence coding for said specific antigen into a cloning site present downstream from a promoter of a vector for expression, introducing the expression vector containing said DNA fragment into a host, culturing said transformed host, and collecting the produced and accumulated antigen.
Claim 15
Dependent Claim
61 words
A process for producing an antigen specific to non-A non-B hepatitis comprising introducing a DNA fragment containing a base sequence coding for said specific antigen into a cloning site present downstream from a promoter of a vector for expression, introducing the expression vector containing said DNA fragment into a host, culturing said transformed host, and collecting the produced and accumulated antigen.
References Cited (Backward Citations)
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