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EP0289121A2
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Mutagenesis testing using transgenic non-human animals carrying test DNA sequences
Assignee: Stratagene California
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Filing: 1988-03-11
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Granted: —
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Expires: 2008-03-11
Abstract
Abstract An assay for monitoring and assessing the mutagenic potential of agents which involves creating transgenic non-human animals carrying a test DNA sequence or sequences that can be quickly recovered and examined for mutations following exposure to one or more suspected mutagenic agents.
Inventors
- 👤 Joseph Anthony Sorge
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 18:16
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; C12Q; C12Q1/00; C12Q1/02; C12Q1/18; C12Q1/68; C12Q1/6897; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/5014; G01N33/5017; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2227/105; A01K2267/00; A01K2267/03; A01K2267/0393
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
35 words
A method for monitoring mutagenic agents comprising creating transgenic non-human animals containing a test DNA sequence, exposing such animals to possible mutagenic agents, and observing the effects of such agents on said test DNA sequence.
Claim 2
Independent Claim
35 words
A method for monitoring mutagenic agents comprising creating transgenic non-human animals containing a test DNA sequence, exposing such animals to possible mutagenic agents, and observing the effects of such agents on said test DNA sequence.
Claim 3
Dependent Claim
32 words
A method in accordance with claim 1 wherein the animal carries a test DNA sequence that may be excised from the animal tissue, inserted into a microorganism, and expressed by the microorganism.
Claim 4
Dependent Claim
66 words
A method in accordance with claim 2 wherein the test DNA sequence is inserted into the single-celled embryos of the animal, the test DNA sequence is carried on a vector containing the cos sites of bacteriophage lambda, the test DNA sequence is integrated into the genome of the host animal, and the test DNA sequence vector allows for expression of the test DNA sequence in microorganisms.
Claim 5
Dependent Claim
68 words
A method in accordance with claim 2 wherein the test DNA sequence is carried by a vector capable of autonomous replication in host animal cells, the test DNA sequence is inserted into the single-celled embryo of the animal, the test DNA sequence autonomously and heritably replicates in the host animal cells, and the test DNA sequence vector allows for expression of the test DNA sequence in a microorganism.
Claim 6
Dependent Claim
65 words
A method in accordance with claim 3 wherein the transgenic non-human animal is a mouse, the test DNA sequence is carried by a modified bacteriophage lambda, the test DNA sequence is integrated into the mouse genome, the test DNA sequence is excised from the mouse genome using a lambda packaging extract, and the test DNA sequence is introduced into and expressed by E. coli .
Claim 7
Dependent Claim
21 words
Transgenic non-human animals containing a test DNA sequence capable of being rescued from the animal cells and expressed by a microorganism.
Claim 8
Dependent Claim
21 words
Transgenic non-human animals containing a test DNA sequence capable of being rescued from the animal cells and expressed by a microorganism.
Claim 9
Dependent Claim
24 words
Transgenic non-human animals in accordance with claim 6 containing a test DNA sequence flanked by the cos ends of a bacteriophage lambda derivative genome.
Claim 10
Dependent Claim
21 words
Transgenic non-human animal embryos containing a test gene capable of being rescued from the animal embryo and expressed by a microorganism.
Claim 11
Dependent Claim
21 words
Transgenic non-human animal embryos containing a test gene capable of being rescued from the animal embryo and expressed by a microorganism.
Claim 12
Dependent Claim
22 words
The transgenic non-human animal resulting from the process of introducing a test gene into a single-celled embryo and differentiation of the embryo.
Claim 13
Dependent Claim
22 words
The transgenic non-human animal resulting from the process of introducing a test gene into a single-celled embryo and differentiation of the embryo.
Claim 14
Dependent Claim
35 words
The transgenic non-human animal of claim 9 wherein the process is comprised of microinjecting a test gene in a lambda vector genome into a single-celled embryo and providing for growth and differentiation of the embryo.
References Cited (Backward Citations)
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