Patent EP0288029A2 successfully refreshed from Google Patents.
EP0288029A2
Expired / Lapsed
EP
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Flow-cell device
Assignee: Hitachi Ltd
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Filing: 1988-04-20
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Granted: —
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Expires: 2008-04-20
Abstract
Abstract A sheath flow type flow-cell device for Flow-Cytometer comprises a first inlet (6) for sheath fluid, a flow passage (9) communicated with the first inlet and contracted toward downstream, the flow passage having a substantially rectangular cross section, a straight capillary flow passage (8) connected to the flow passage at downstream thereof, the capillary flow passage having a substantially rectangular cross section, a second inlet (7) for sample fluid, a nozzle (10) communicated with the second inlet and opened within the flow pasage in the same direction as the flow direction of the straight capillary flow passage, a discharge port (3) provided at a terminal end of the straight capillary flow passage, and flow regulating means (8a, 8b; 19; 20) for regulating the flow of the sheath fluid in the straight capillary flow passage to be a laminar flow having a gradient of flow velocity.
Inventors
- 👤 Hiroshi Ohki
- 👤 Hideaki Kamohara
- 👤 Ryo Miyake
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-27 21:04
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1404
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
146 words
A sheath flow type flow-cell device for Flow-Cytometer comprising a first inlet (6) for sheath fluid, a flow passage (9) communicated with said first inlet and contracted toward downstream, said flow passage having a substantially rectangular cross section, a straight capillary flow passage (8) connected to said flow passage at downstream thereof, said capillary flow passage having a substantially rectangular cross section, a second inlet (7) for sample fluid, a nozzle (10) communicated with said second inlet and opened within said flow passage in the same direction as the flow direction of said straight capillary flow passage, a discharge port (3) provided at a terminal end of said straight capillary flow passage, characterized by flow regulating means (8a, 8b; 19; 20) for regulating the flow of said sheath fluid in said straight capillary flow passage to be a laminar flow having a gradient of flow velocity.
Claim 2
Dependent Claim
146 words
A sheath flow type flow-cell device for Flow-Cytometer comprising a first inlet (6) for sheath fluid, a flow passage (9) communicated with said first inlet and contracted toward downstream, said flow passage having a substantially rectangular cross section, a straight capillary flow passage (8) connected to said flow passage at downstream thereof, said capillary flow passage having a substantially rectangular cross section, a second inlet (7) for sample fluid, a nozzle (10) communicated with said second inlet and opened within said flow passage in the same direction as the flow direction of said straight capillary flow passage, a discharge port (3) provided at a terminal end of said straight capillary flow passage, characterized by flow regulating means (8a, 8b; 19; 20) for regulating the flow of said sheath fluid in said straight capillary flow passage to be a laminar flow having a gradient of flow velocity.
Claim 3
Dependent Claim
39 words
A sheath flow type flow-cell device as claimed in claim 1, wherein said flow regulating means comprises a side wall (8a) of said capillary flow passage having a smooth surface and another side wall (8b) having a rough surface.
Claim 4
Dependent Claim
54 words
A sheath flow type flow-cell device as claimed in claim 2, wherein said flow regulating means further includes a part (17) of a top wall (15) and a part (18) of a bottom wall (16) of said capillary flow passage having rough surface portions adjacent to said another side wall having a rough surface.
Claim 5
Dependent Claim
52 words
A sheath flow type flow-cell device as claimed in claim 2, wherein the surface roughness of said smooth surface is 1/500 and under of a distance between said side wall (8a) and said another side wall (8b) and the surface roughness of said rough surface is 1/20 and over of said distance.
Claim 6
Dependent Claim
37 words
A sheath flow type flow-cell device as claimed in claim 2, wherein the surface roughness of said smooth surface exists from 1S to 10S and the surface roughness of said rough surface exists from 100S to 1000S.
Claim 7
Dependent Claim
52 words
A sheath flow type flow-cell device as claimed in claim 3, wherein the width of said rough surfaces (17, 18) of said top and bottom walls (15, 16) from said another side wall (8b) is 1/3 and under of a distance between said side wall (8a) and said another side wall (8b).
Claim 8
Dependent Claim
60 words
A sheath flow type flow-cell device as claimed in claim 1, wherein said flow regulating means comprises a net member (19) stretched across said flow passage at upstream from the opening of said nozzle, the mesh of said net being minuter from a side wall (21) of said flow passage toward another side wall (22) opposite to said side wall.
Claim 9
Dependent Claim
74 words
A sheath flow type flow-cell device as claimed in claim 1, wherein said flow regulating means comprises a plurality of partition walls (20) extended in said flow passage in the flow direction and dividing said flow passage into a plurality of divided flow passages (23a, 23b, 23c, 23d, 23e, 23f) of which flow resistance are made larger from a side wall of said flow passage toward another side wall opposite to said side wall.
References Cited (Backward Citations)
No citation records indexed.
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