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EP0286195A2
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Method and apparatus for detecting low concentrations of (bio)- chemical components in a test medium using surface plasmon resonance
Assignee: Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
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Filing: 1988-04-07
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Granted: —
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Expires: 2008-04-07
Abstract
Abstract A method and an apparatus for detecting low concentrations of at least one (bio-)chemical component present in a test medium in a test cell, having a metal layer as sub wall with an external glass prism, using the surface plasmon resonance effect. A light ray is coupled in and, after attenuated total reflection, is coupled out and the intensity thereof is measured. The incidence angle position of the resonance curve is determined under the influence of the change, caused by the component, in the dielectric constant of the test medium near the metal layer. An adjustable selector is applied to the metal layer, in order to influence the incidence angle position of the resonance curve, through which the concentrations or concentration changes of one or more components in the test medium can be simultanously determined through one or more differential measurements. A preferential association and therefor a higher concentration at the metal layer of one component above another is brought about.
Inventors
- 👤 Jacobus Johannes Frederick Van Veen
- 👤 Johan Willem König
- 👤 Willem Maurits Ter Kuile
- 👤 Cornelis Van Dijk
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 13:35
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/55; G01N21/552; G01N21/553; G01N30/00; G01N30/02; G01N30/62; G01N30/74; Y; Y10; Y10S; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
174 words
A method for detecting low concentrations of at least one (bio-)chemical component present in a test medium in a test cell, having a metal layer as sub wall with an external glass prism, using the surface plasmon resonance effect, in which a light ray polarized in the plane of incidence is coupled in via the prism and, after attenuated total reflection, is coupled out and the intensity thereof is measured, the incidence angle position of the resonance curve herein being determined under the influence of the change, caused by the component, in the dielectric constant of the test medium near the metal layer, characterized in that an adjustable selector is applied to the metal layer, in order to influence the incidence angle position of the resonance curve, through which the concentrations or concentration changes of one or more components in the test medium can be simultanously determined through one or more differential measurements because a preferential association and therefor a higher concentration at the metal layer of one component above another is brought about.
Claim 2
Independent Claim
174 words
A method for detecting low concentrations of at least one (bio-)chemical component present in a test medium in a test cell, having a metal layer as sub wall with an external glass prism, using the surface plasmon resonance effect, in which a light ray polarized in the plane of incidence is coupled in via the prism and, after attenuated total reflection, is coupled out and the intensity thereof is measured, the incidence angle position of the resonance curve herein being determined under the influence of the change, caused by the component, in the dielectric constant of the test medium near the metal layer, characterized in that an adjustable selector is applied to the metal layer, in order to influence the incidence angle position of the resonance curve, through which the concentrations or concentration changes of one or more components in the test medium can be simultanously determined through one or more differential measurements because a preferential association and therefor a higher concentration at the metal layer of one component above another is brought about.
Claim 3
Dependent Claim
36 words
A method according to claim 1, characterized in that the adjustable selector is a variable potential through which in the differential measurement(s) a potential-dependent preferential adsorption of the one component above the other is brought about.
Claim 4
Dependent Claim
66 words
A method according to claim 1, characterized in that various adjustable potentials are applied to an array of metal layer strips in the sub wall, in that several light rays corresponding with the strips are coupled in simultaneously, and that said light rays reflected by the separate metal layer strips are measured, as a result of which one or more differential measurements can be carried out.
Claim 5
Dependent Claim
81 words
A method according to claim 2, characterized in that a single beam of parallel light rays is coupled in under a fixed angle of incidence, the interface metal layer/test medium being provided with a plurality of parallel grooves, the longitudinal direction of which being transverse to the direction of the beam of light rays such that a fan of light rays, coupled out under different angles, comes about, the intensity of which rays is measured by an array of light detectors.
Claim 6
Dependent Claim
52 words
A method according to claim 1, characterized in that the adjustable selector is a plurality of different (bio-)chemical affinity ligands which are fixedly adsorbed in an array to the metal layer, through whih in the differential measurement(s) an affinity ligand-dependent preferential binding of the one component above the other is brought about.
Claim 7
Independent Claim
204 words
An apparatus for detecting low concentrations of at least one (bio-)chemical component present in a test medium using the surface plasmon resonance effect, which apparatus is provided with a test cell for the test medium having a metal layer as sub wall with an external prism, a monochromatic light source for coupling in via the prism a light ray polarized in the plane of incidence in order to excite a surface plasmon wave in the metal layer, and at least a detector for measuring the intensity occurring with attenuated total reflection in the light ray coupled out, the incidence angle position of the reference curve therein changing under the influence of the change, caused by the component, in the dielectric constant of the test medium near the metal layer, characterized in that the test cell is embodied with an adjustable selector at the metal layer in order to influence the incidence angle position of the resonance curve, through which the concentrations or concentration changes of one or more components in the test medium can be simultaneously determined via one or more differential measurements because a preferential association and thereby a higher concentration at the metal layer of one component above another is brought about.
Claim 8
Independent Claim
204 words
An apparatus for detecting low concentrations of at least one (bio-)chemical component present in a test medium using the surface plasmon resonance effect, which apparatus is provided with a test cell for the test medium having a metal layer as sub wall with an external prism, a monochromatic light source for coupling in via the prism a light ray polarized in the plane of incidence in order to excite a surface plasmon wave in the metal layer, and at least a detector for measuring the intensity occurring with attenuated total reflection in the light ray coupled out, the incidence angle position of the reference curve therein changing under the influence of the change, caused by the component, in the dielectric constant of the test medium near the metal layer, characterized in that the test cell is embodied with an adjustable selector at the metal layer in order to influence the incidence angle position of the resonance curve, through which the concentrations or concentration changes of one or more components in the test medium can be simultaneously determined via one or more differential measurements because a preferential association and thereby a higher concentration at the metal layer of one component above another is brought about.
Claim 9
Dependent Claim
37 words
An apparatus according to claim 6, characterized in that the adjustable selector is a variable potential which is applied between a reference electrode and the metal layer, embodied as a working electrode, with respect to a counterelectrode.
Claim 10
Dependent Claim
74 words
An apparatus according to claim 7, characterized in that the metal layer is constructed as an array of metal layer strips, various potentials being applied to the separate metal layer strips with respect to the reference electrode, and in that the light source and the detector are constructed respectively as a corresponding light source array and photo diode array, a plurality of light rays being coupled in simultaneously and being coupled out after reflection.
Claim 11
Dependent Claim
33 words
An apparatus according to claim 6, characterized in that the adjustable selector at the metal layer is formed by an array of (bio-)chemical affinity ligands which are fixedly adsorped to the metal layer.
Claim 12
Dependent Claim
37 words
An apparatus according to claim 9, characterized in that the array of (bio-)chemical affinity ligands are a number of different antibodies or antigenes, such that in the test medium various antigenes or antibodies respectively can be determined.
Claim 13
Dependent Claim
34 words
An apparatus according to claim 9, characterized in that the array of (bio-)chemical affinity ligands are a number of different DNA- or RNA-probes such that in the test medium homologous DNA can be determined.
Claim 14
Dependent Claim
96 words
An apparatus according to claim 7, characterized in that the light source emits a beam of parallel light rays to the prism, which beam is coupled in under a fixed angle, that in the test cell the interface metal layer/test medium is embodied with a plurality of parallel grooves, the longitudinal direction of which is transverse to the direction of the beam of light rays, such that a fan of light rays coupled out under different angles come about, and that an array of detectors is provided to measure the intensity of the coupled-out light rays.
Claim 15
Dependent Claim
28 words
An apparatus according to claim 12, characterized in that the angle range of the fan of light rays, coupled out under different angles, is between 60° and 70°.
Claim 16
Dependent Claim
46 words
An apparatus according to claim 12 or 13, characterized in that by simultaneous irradiation of two or more grooves the coupled-out light rays constitute a characteristic interference pattern, which can be used as internal calibration to assist in determining the shift of the plasmon resonance curve.
References Cited (Backward Citations)
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