Patent EP0276846A2 successfully refreshed from Google Patents.
EP0276846A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Colony-stimulating factor derivatives
Assignee: University of Washington
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Filing: 1988-01-28
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Granted: —
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Expires: 2008-01-28
Abstract
Abstract Novel proteins possessing substantially the same biological activity as human GM-CSF, as well as proteins having a higher specific activity than native human GM-CSF, are disclosed. The proteins may (a) be unglycosylated; (b) lack N-linked glycosylation but have O-linked glycosylation; or (c) lack one of the two N-linked carbohydrate chains characteristic of native human GM-CSF. The proteins are produced using recombinant DNA techniques in eucaryotic host cells, such as mammalian, yeast, and filamentous fungal host cells. Pharmaceutical compositions including an effective amount of one of the proteins of the invention and a physiologically acceptable carrier or diluent are also disclosed.
Inventors
- 👤 Frederick S. Hagen
- 👤 Kenneth Kaushansky
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 21:37
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; A; A61; A61P; A61P37/00; A61P37/02; A61P37/04; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/53; C07K14/535; A61K; A61K38/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
19 words
A protein lacking N-linked glycosylation but having O-linked glycosylation, said protein having higher specific activity than native human GM-CSF.
Claim 2
Dependent Claim
19 words
A protein lacking N-linked glycosylation but having O-linked glycosylation, said protein having higher specific activity than native human GM-CSF.
Claim 3
Dependent Claim
18 words
A protein having only one N-linked carbohydrate chain, said protein having higher specific activity than native human GM-CSF.
Claim 4
Dependent Claim
18 words
A protein having only one N-linked carbohydrate chain, said protein having higher specific activity than native human GM-CSF.
Claim 5
Dependent Claim
31 words
A protein having the amino acid sequence of Figure l, starting with alanine, number l8, and ending with glutamate, number l44, said protein having higher specific activity than native human GM-CSF.
Claim 6
Dependent Claim
31 words
A protein having the amino acid sequence of Figure l, starting with alanine, number l8, and ending with glutamate, number l44, said protein having higher specific activity than native human GM-CSF.
Claim 7
Dependent Claim
23 words
The protein of claim 3 wherein said protein is encoded by the DNA sequence of Figure l, from bp 52 to bp 432.
Claim 8
Dependent Claim
31 words
A protein having the amino acid sequence of Figure 2, starting with alanine, number l8, and ending with glutamate, number l44, said protein having higher specific activity than native human GM-CSF.
Claim 9
Dependent Claim
31 words
A protein having the amino acid sequence of Figure 2, starting with alanine, number l8, and ending with glutamate, number l44, said protein having higher specific activity than native human GM-CSF.
Claim 10
Dependent Claim
23 words
The protein of claim 5 wherein said protein is encoded by the DNA sequence of Figure 2, from bp 52 to bp 432.
Claim 11
Dependent Claim
31 words
A protein having the amino acid sequence of Figure 3, starting with alanine, number l8, and ending with glutamate, number l44, said protein having higher specific activity than native human GM-CSF.
Claim 12
Dependent Claim
31 words
A protein having the amino acid sequence of Figure 3, starting with alanine, number l8, and ending with glutamate, number l44, said protein having higher specific activity than native human GM-CSF.
Claim 13
Dependent Claim
23 words
The protein of claim 7 wherein said protein is encoded by the DNA sequence of Figure 3, from bp 52 to bp 432.
Claim 14
Independent Claim
78 words
A method for producing a protein having higher specific activity than native human GM-CSF, comprising: introducing into a eucaryotic host cell an expression unit comprising a promoter followed downstream by a DNA sequence encoding a protein according to any of claims l-8, said protein having higher specific activity than native human GM-CSF; growing said eucaryotic host cell in an appropriate medium; and isolating the protein product encoded by said DNA sequence and produced by said eucaryotic host cell.
Claim 15
Dependent Claim
24 words
l0. The method of claim 9 wherein said eucaryotic host cell is selected from the group consisting of mammalian, yeast, and Aspergillus host cells.
Claim 16
Dependent Claim
33 words
A pharmaceutical composition comprising an effective amount of a protein according to any of claims l-8, said protein having higher specific activity than native human GM-CSF, and a physiologically acceptable carrier or diluent.
Claim 17
Dependent Claim
15 words
A protein according to any of claim l-8, for use as an active therapeutic substance.
References Cited (Backward Citations)
No citation records indexed.
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