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EP0273125A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

DNA fragments encoding alkaline cellulase, and plasmids containing them

Assignee: Kao Corp
•
Filing: 1987-10-21
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Granted: —
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Expires: 2007-10-21
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Abstract

Abstract DNA fragments containing an alkaline cellulase K gene, which comprise about 4.0, about 2.4 and about l.9 kilo base pairs, respectively, each having a specific restriction map, recombinant plasmids containing any of these DNA fragments, and recombinant microorganisms harboring any of said plasmids. The alkaline cellulase K gene is derived from a bacterial strain which belongs to the genus Bacillus and is alkalophilic, that is, capable of revealing an optimum growth in an alkaline pH region.

Inventors

  • 👤 Katsuya Ozaki
  • 👤 Kazushi Oshino
  • 👤 Kenzo Koike
  • 👤 Susumu Ito
  • 👤 Kikuhiko Okamoto

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-05 02:46
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004

Claims Analysis

Total Claims: 15
Claim 1
Independent Claim 8 words
A DNA fragment coding for alkaline cellulase K.
Claim 2
Dependent Claim 8 words
A DNA fragment coding for alkaline cellulase K.
Claim 3
Dependent Claim 22 words
A DNA fragment of Claim l, which comprises about 4.0 kilo base pairs and has the restriction map shown in Fig. l.
Claim 4
Dependent Claim 22 words
A DNA fragment of Claim l, which comprises about 2.4 kilo base pairs and has the restriction map shown in Fig. 2.
Claim 5
Dependent Claim 22 words
A DNA fragment of Claim l, which comprises about l.9 kilo base pairs and has the restriction map shown in Fig. 3.
Claim 6
Dependent Claim 13 words
A recombinant plasmid which contains a DNA fragment coding for alkaline cellulase K.
Claim 7
Dependent Claim 13 words
A recombinant plasmid which contains a DNA fragment coding for alkaline cellulase K.
Claim 8
Dependent Claim 25 words
A recombinant plasmid of Claim 5, wherein said DNA fragment comprises about 4.0 kilo base pairs and has the restriction map shown in Fig. l.
Claim 9
Dependent Claim 25 words
A recombinant plasmid of Claim 5, wherein said DNA fragment comprises about 2.4 kilo base pairs and has the restriction map shown in Fig. 2.
Claim 10
Dependent Claim 24 words
A recombinant plasmid of Claim 5, wherein said DNA fragment comprises about l.9 kilo base pairs has the restriction map shown in Fig. 3.
Claim 11
Dependent Claim 17 words
A recombinant microorganism carrying a recombinant plasmid which contains a DNA fragment coding for alkaline cellulase K.
Claim 12
Dependent Claim 17 words
A recombinant microorganism carrying a recombinant plasmid which contains a DNA fragment coding for alkaline cellulase K.
Claim 13
Dependent Claim 26 words
l0. A recombinant microorganism of Claim 9, wherein said DNA fragment comprises about 4.0 kilo base pairs and has the restriction map shown in Fig. l.
Claim 14
Dependent Claim 25 words
A recombinant microorganism of Claim 9, wherein said DNA fragment comprises about 2.4 kilo base pairs and has the restriction map shown in Fig. 2.
Claim 15
Dependent Claim 25 words
A recombinant microorganism of Claim 9, wherein said DNA fragment comprises about l.9 kilo base pairs and has the restriction map shown in Fig. 3.

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