Patent US6084468A successfully refreshed from Google Patents.
Patent EP0270355A2 successfully refreshed from Google Patents.
EP0270355A2
Expired / Lapsed
EP
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Genetic engineering of cotton plants and lines
Assignee: Monsanto Technology LLC
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Filing: 1987-12-02
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Granted: —
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Expires: 2007-12-02
Abstract
Abstract A method is disclosed to achieve genetic transformation of cotton plants and lines. Immature cotton tissues are genetically transformed in vitro, by Agrobacterium -mediated genetic transformation. The resultant cotton tissues are subjected to a selection agent or agents to screen for transformants. The transformed cultures are then induced to commence somatic embryogenesis. One possible regime for regenerating such somatic embryos into whole cotton plants is disclosed.
Inventors
- 👤 Paul F. Umbeck
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 14:21
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8201; C12N15/8202; C12N15/8205
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
52 words
A method of introducing genes into cotton plants and plant lines characterised in that it comprises exposing hypocotyl tissue of immature cotton plants to a culture of transformation competent non-oncogenic Agrobacterium tumefaciens harboring a Ti plasmid having a T-DNA region including both a foreign chimeric gene and a selection agent resistance gene.
Claim 2
Independent Claim
52 words
A method of introducing genes into cotton plants and plant lines characterised in that it comprises exposing hypocotyl tissue of immature cotton plants to a culture of transformation competent non-oncogenic Agrobacterium tumefaciens harboring a Ti plasmid having a T-DNA region including both a foreign chimeric gene and a selection agent resistance gene.
Claim 3
Dependent Claim
63 words
A method as claimed in claim 1, characterised in that it further comprises culturing the exposed tissue in the presence of a selection agent for which the resistance gene encodes for resistance so as to select for plant cells transformed with the T-DNA region; inducing somatic embryo formation in the exposed tissue in culture; and regenerating the somatic embryos into whole cotton plants.
Claim 4
Dependent Claim
38 words
A method as claimed in either claim 1 or claim 2, characterised in that said exposing step is preceded by surface sterilization of cotton seeds followed by germination of said cotton seeds to form said immature cotton plants.
Claim 5
Independent Claim
30 words
A method as claimed in any one of the preceding claims, characterised in that the hypocotyl tissue comprises pieces of hypocotyl explants which are removed from said immature cotton plants.
Claim 6
Independent Claim
43 words
A method as claimed in any one of the preceding claims, characterised in that the culture of Agrobacterium tumefaciens harbors a binary Ti plasmid system in which a virulence trait is carried on a plasmid separate from the plasmid carrying the T-DNA region.
Claim 7
Dependent Claim
29 words
A method as claimed in claim 5, characterised in that the T-DNA region includes only the T-DNA right and left borders from the T-DNA of a wild-type Ti plasmid.
Claim 8
Independent Claim
29 words
A method as claimed in any one of claims 2 to 6, characterised in that the selection agent is an antibiotic and the resistance gene codes for antibiotic resistance.
Claim 9
Dependent Claim
31 words
A method as claimed in claim 7, characterised in that the antibiotic resistance gene is the NPTII gene and the antibiotic is selected from the group consisting of Kanamycin and G418.
Claim 10
Dependent Claim
23 words
A method as claimed in claim 7, characterised in that the antibiotic resistance gene is the CAT gene and the antibiotic is Chloramphenicol.
Claim 11
Dependent Claim
38 words
A method as claimed in claim 7, characterised in that two antibiotics and two antibiotic resistance genes are used, said antibiotics being preferably selected from the group consisting of Hygromycin B, Chloramphenicol, and one of Kanamycin and G418.
Claim 12
Independent Claim
54 words
A method as claimed in any one of the preceding claims, characterised in that it further includes, after the step of exposing the tissue to said Agrobacterium tumefaciens , a step of culturing the tissue on a medium containing at least one antibiotic toxic to said Agrobacterium tumefaciens but not toxic to cotton cells.
Claim 13
Independent Claim
37 words
A method as claimed in any one of claims 2 to 11, characterised in that the step of inducing embryo formation includes culturing the plant tissue on a culture media containing at least one auxin or cytokinin.
Claim 14
Dependent Claim
20 words
Cotton plants characterised in that they are produced by a method as claimed in any one of the preceding claims.
Claim 15
Dependent Claim
22 words
Cotton somatic embryos characterised in that they are produced by a method as claimed in any one of claims 1 to 12.
Claim 16
Dependent Claim
15 words
Cotton seeds characterised in that they are produced by plants as claimed in claim 13.
Claim 17
Dependent Claim
52 words
Cotton seeds capable of germination into cotton plants comprising in their genome a chimeric gene construction including a foreign gene and promoter and control sequences operable in plant cells, the chimeric gene construction being effective in the cells of the cotton plant to express a cellular product coded by the foreign gene.
Claim 18
Dependent Claim
52 words
Cotton seeds capable of germination into cotton plants comprising in their genome a chimeric gene construction including a foreign gene and promoter and control sequences operable in plant cells, the chimeric gene construction being effective in the cells of the cotton plant to express a cellular product coded by the foreign gene.
Claim 19
Dependent Claim
30 words
Cotton seeds as claimed in claim 16, characterised in that the cellular product is an exogenous protein or an RNA capable of producing a somatic change to the cotton plant.
Claim 20
Dependent Claim
36 words
Cotton seeds as claimed in claim 16, characterised in that the foreign gene codes for the production of a negative RNA strand effective to condition a somatic change in the cotton plant grown from the seed.
Claim 21
Dependent Claim
33 words
Cotton seeds as claimed in any one of claims 16 to 18, characterised in that the promoter sequence is the nopaline synthase promoter from Agrobacterium tumefaciens or the cauliflower mosaic virus 35s promoter.
Claim 22
Dependent Claim
20 words
Cotton plants characterised in that they are germinated from seeds as claimed in any one of claims 16 to 19.
References Cited (Backward Citations)
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