EP0259157A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Immunoseparating strip
Assignee: Abbott Laboratories
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Filing: 1987-09-03
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Granted: —
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Expires: 2007-09-03
Abstract
Abstract A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a piece of bibulous material capable of being traversed in at least one direction by the test solution through capillary action. The bibulous material contains a first receptor capable of binding to said conjugate. The first receptor is non-diffusively bound to a situs on the bibulous material separate from the contact portion. The bibulous material further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the bibulous material. At least a portion of the test solution is allowed to traverse the bibulous material by capillary action and thereby contact the situs. The situs is examined for the presence of the conjugate. To this end, the situs can be exposed to a signal producing means capable of interacting with the label to produce a signal in relation to the amount of analyte in the test solution. The signal produced at the situs is then detected.
Inventors
- 👤 John D. Olson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 05:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/973; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/816
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
184 words
A method for determining the presence of an analyte that is capable of binding specifically to an antibody in a sample suspected of containing said analyte, which comprises - (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a piece of bibulous material capable of being traversed in at least one direction by said test solution by capillary migration, said bibulous material containing non-diffusively bound to a situs on said bibulous material separate from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said bibulous material, said bibulous material further containing a second receptor capable of binding said antibodies to said analyte non-diffusively bound to said bibulous material at a portion thereof between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said bibulous material by capillary migration and thereby contact said situs, and (c) examining said situs for the presence of said conjugate.
Claim 2
Independent Claim
184 words
A method for determining the presence of an analyte that is capable of binding specifically to an antibody in a sample suspected of containing said analyte, which comprises - (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a piece of bibulous material capable of being traversed in at least one direction by said test solution by capillary migration, said bibulous material containing non-diffusively bound to a situs on said bibulous material separate from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said bibulous material, said bibulous material further containing a second receptor capable of binding said antibodies to said analyte non-diffusively bound to said bibulous material at a portion thereof between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said bibulous material by capillary migration and thereby contact said situs, and (c) examining said situs for the presence of said conjugate.
Claim 3
Independent Claim
196 words
A method for determining the presence of an analyte that is capable of binding specifically to an antibody in a sample suspected of containing said analyte, which comprises - (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing non-diffusively bound to a situs on said strip separate from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said strip, said strip further containing a second receptor capable of binding said antibodies to said analyte non-diffusively bound to said strip between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs, (c) exposing said strip to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution, and (d) detecting said signal at said situs.
Claim 4
Independent Claim
196 words
A method for determining the presence of an analyte that is capable of binding specifically to an antibody in a sample suspected of containing said analyte, which comprises - (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing non-diffusively bound to a situs on said strip separate from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said strip, said strip further containing a second receptor capable of binding said antibodies to said analyte non-diffusively bound to said strip between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs, (c) exposing said strip to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution, and (d) detecting said signal at said situs.
Claim 5
Dependent Claim
15 words
The method of Claim 1 or 2 wherein said bibulous material is a paper strip.
Claim 6
Dependent Claim
68 words
The method of Claim 1 wherein said situs is examined for the presence of a signal indicating the presence of said conjugate, and wherein either said situs is exposed to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution or wherein said situs is examined directly for the presence of a signal.
Claim 7
Dependent Claim
25 words
The method of Claim 4 wherein said signal at said situs is compared with signal at a portion of the strip other than said situs.
Claim 8
Dependent Claim
31 words
The method of Claim 2 or 4 wherein when said situs is exposed to a signal producing system, said signal producing means comprises a substrate and said label is a catalyst.
Claim 9
Dependent Claim
24 words
The method of Claim 1 or Claim 2 wherein said first receptor is an antibody, and said second receptor is antibody for said antibodies.
Claim 10
Dependent Claim
42 words
The method of Claim 1 or Claim 2 wherein said label is an enzyme, preferably wherein a second enzyme is bound to said bibulous material, the enzymes being related in that the product of one enzyme is the substrate of the other.
Claim 11
Dependent Claim
14 words
The method of Claim 1 or Claim 2 wherein said analyte is a drug.
Claim 12
Independent Claim
223 words
A method for determining the presence of one or more of a plurality of analytes, each capable of binding specifically to an antibody, in a sample suspected of containing one or more of said analytes, which comprises - (a) contacting, with a test solution containing said sample, one or more of a plurality of antibodies each respectively complementary to one of said analytes, and one or more of a plurality of conjugates of one of said analytes and a label, a contact portion of a piece of bibulous material capable of being traversed by said test solution by capillary migration, said bibulous material containing non-diffusively bound to a situs on said bibulous material separate from said contact portion one or more of a plurality of first receptors each respectively capable of binding to one of said conjugates, the surface area of said situs being less than that of said bibulous material, said bibulous material further containing one or more second receptors, capable of binding all of said antibodies to said analytes, non-diffusively bound to said bibulous material at least at a portion thereof between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs, and (c) examining said situs for the presence of said conjugate.
Claim 13
Independent Claim
223 words
A method for determining the presence of one or more of a plurality of analytes, each capable of binding specifically to an antibody, in a sample suspected of containing one or more of said analytes, which comprises - (a) contacting, with a test solution containing said sample, one or more of a plurality of antibodies each respectively complementary to one of said analytes, and one or more of a plurality of conjugates of one of said analytes and a label, a contact portion of a piece of bibulous material capable of being traversed by said test solution by capillary migration, said bibulous material containing non-diffusively bound to a situs on said bibulous material separate from said contact portion one or more of a plurality of first receptors each respectively capable of binding to one of said conjugates, the surface area of said situs being less than that of said bibulous material, said bibulous material further containing one or more second receptors, capable of binding all of said antibodies to said analytes, non-diffusively bound to said bibulous material at least at a portion thereof between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs, and (c) examining said situs for the presence of said conjugate.
Claim 14
Independent Claim
143 words
A device for determining the presence of an analyte that is capable of binding specifically to an antibody in a test solution comprised of an antibody for said analyte, a conjugate of said analyte and a label, and a sample suspected of containing the analyte, said device comprising - a piece of bibulous material capable of traversal by said test solution by capillary migration, said bibulous material having a contact portion for contacting said test solution and a first receptor for said conjugate non-diffusively bound to a situs on said bibulous material separated from said contact portion, the surface area of said situs being less than that of said bibulous material, said bibulous material further containing a second receptor capable of binding said antibodies non-diffusively bound to said bibulous material at least at a portion thereof between said situs and said contact portion.
Claim 15
Independent Claim
143 words
A device for determining the presence of an analyte that is capable of binding specifically to an antibody in a test solution comprised of an antibody for said analyte, a conjugate of said analyte and a label, and a sample suspected of containing the analyte, said device comprising - a piece of bibulous material capable of traversal by said test solution by capillary migration, said bibulous material having a contact portion for contacting said test solution and a first receptor for said conjugate non-diffusively bound to a situs on said bibulous material separated from said contact portion, the surface area of said situs being less than that of said bibulous material, said bibulous material further containing a second receptor capable of binding said antibodies non-diffusively bound to said bibulous material at least at a portion thereof between said situs and said contact portion.
Claim 16
Dependent Claim
42 words
A kit for use in determining the presence of an analyte in a test solution, comprising in a packaged combination-- (a) an antibody for said analyte, (b) a conjugate of said analyte and a label, and (c) the device of Claim 11.
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