Patent EP0255378A2 successfully refreshed from Google Patents.
EP0255378A2
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EP
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Seed specific transcriptional regulation
Assignee: Monsanto Co
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Filing: 1987-07-30
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Granted: —
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Expires: 2007-07-30
Abstract
Abstract Nucleic acid sequences and methods for their use are provided which provide for seed specific transcription, in order to modulate or modify expression in seed, particularly embryo cells. Transcriptional initiation regions are identified and isolated from plant cells and used to prepare expression cassettes which may then be transformed into plant cells for seed specific transcription. The method finds particular use in conjunction with modifying fatty acid production in seed tissue.
Inventors
- 👤 Jean C. Kridl
- 👤 Vic C. Knauf
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 06:03
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/415; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N15/823; C12N15/8234
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
53 words
A seed comprising an expression cassette, said cassette comprising a seed specific transcriptional initiation region, a sequence of interest under the transcriptional regulation of said initiation region, and a transcriptional termination region, said expression cassette inserted into the genome of said seed at other than the natural site for said transcriptional initiation region.
Claim 2
Dependent Claim
53 words
A seed comprising an expression cassette, said cassette comprising a seed specific transcriptional initiation region, a sequence of interest under the transcriptional regulation of said initiation region, and a transcriptional termination region, said expression cassette inserted into the genome of said seed at other than the natural site for said transcriptional initiation region.
Claim 3
Dependent Claim
23 words
A seed according to Claim 1, wherein said sequence of interest is an open reading frame encoding an endogenous protein or mutant thereof.
Claim 4
Dependent Claim
20 words
A seed according to Claim 1, wherein said sequence of interest is an open reading frame encoding an exogenous protein.
Claim 5
Dependent Claim
22 words
A seed according to Claim 1, wherein said sequence of interest encodes a sequence complementary to a transcription product of said seed.
Claim 6
Dependent Claim
14 words
A seed according to Claim 1, wherein said seed is of the Brassica family.
Claim 7
Dependent Claim
67 words
An expression construct comprising a seed specific transcriptional initiation region, a polylinker of less than about 100 bp having at least two restriction sites for insertion of a DNA sequence to be under the transcriptional control of said initiation region, and a transcriptional termination region, the sequence of said polylinker being other than the sequence of the gene naturally under the transcriptional control of said initiation region.
Claim 8
Dependent Claim
67 words
An expression construct comprising a seed specific transcriptional initiation region, a polylinker of less than about 100 bp having at least two restriction sites for insertion of a DNA sequence to be under the transcriptional control of said initiation region, and a transcriptional termination region, the sequence of said polylinker being other than the sequence of the gene naturally under the transcriptional control of said initiation region.
Claim 9
Dependent Claim
18 words
An expression construct according to Claim 6, wherein said transcription initiation region is from a Brassica seed gene.
Claim 10
Dependent Claim
14 words
An expression construct according to Claim 7, wherein said gene is a napin gene.
Claim 11
Dependent Claim
40 words
An expression cassette comprising a seed specific transcriptional initiation region, a DNA sequence of interest, other than the natural sequence joined to said initiation region, to be under the transcriptional control of said initiation region, and a transcriptional termination region.
Claim 12
Dependent Claim
40 words
An expression cassette comprising a seed specific transcriptional initiation region, a DNA sequence of interest, other than the natural sequence joined to said initiation region, to be under the transcriptional control of said initiation region, and a transcriptional termination region.
Claim 13
Dependent Claim
18 words
An expression cassette according to Claim 9, wherein said transcriptional initiation region is a Brassica gene initiation region.
Claim 14
Dependent Claim
14 words
An expression cassette according to Claim 10, wherein said gene is a napin gene.
Claim 15
Dependent Claim
16 words
An expression cassette according to Claim 10, wherein said sequence of interest is a structural gene.
Claim 16
Dependent Claim
22 words
An expression cassette according to Claim 12, wherein said structural gene encodes a protein in the biosynthetic pathway for fatty acid production.
Claim 17
Dependent Claim
14 words
An expression cassette according to Claim 13, wherein said protein is acyl carrier protein.
Claim 18
Dependent Claim
29 words
A vector comprising an expression cassette according to any of Claims 9 to 14, a prokaryotic replication system, and a marker for selection of transformed prokaryotes comprising said marker.
Claim 19
Independent Claim
40 words
A method for modifying the genotype of a seed comprising: growing a plant to seed production, wherein cells of said plant comprise an expression cassette according to any of Claims 9 to 14, whereby seeds are produced of modified genotype.
References Cited (Backward Citations)
No citation records indexed.
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