Patent EP0255087A2 successfully refreshed from Google Patents.
EP0255087A2
Expired / Lapsed
EP
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Cobalt(III) reagents in combination with water soluble polymers
Assignee: Eastman Kodak Co
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Filing: 1987-07-27
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Granted: —
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Expires: 2007-07-27
Abstract
Abstract Water soluble polymers are useful in combination with cobalt(III) redox reagents. When incorporated with the polymers, the cobalt(III) reagents exhibit improved sensitivity compared to fresh solution of the reagents. In preferred embodiments, the cobalt(III) reagents are coated on a support using the water soluble polymer as a binder. Small areas of the coated support are then cut out and placed in a buffer solution to provide a working reagent solution. The solution is particularly useful in the testing of urine for significant bacteriuria.
Inventors
- 👤 Brent Arthur C/O Eastman Kodak Company Burdick
- 👤 Robert William C/O Eastman Kodak Company Zercie
- 👤 Eric Richard C/O Eastman Kodak Company Schmittou
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 20:27
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; G; G01; G01N; G01N31/00; G01N31/22; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C12Q2304/00; C12Q2304/20; C12Q2304/26
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
101 words
A reagent composition for the detection of an analyte in an aqueous sample characterized in that it comprises: (1) a water soluble cobalt(III) complex which is capable of being reduced to a water soluble cobalt(II) complex by said analyte and, (2) a water soluble dye which is capable of being metallized by said cobalt(II) complex to form a water soluble cobalt(II) complex of the metallizable dye, wherein said cobalt(II) complex of the metallizable dye is capable of reacting with said cobalt(III) complex to produce a cobalt(III) complex of the metallizable dye and the cobalt(II) complex; and (3) a water soluble polymer.
Claim 2
Dependent Claim
101 words
A reagent composition for the detection of an analyte in an aqueous sample characterized in that it comprises: (1) a water soluble cobalt(III) complex which is capable of being reduced to a water soluble cobalt(II) complex by said analyte and, (2) a water soluble dye which is capable of being metallized by said cobalt(II) complex to form a water soluble cobalt(II) complex of the metallizable dye, wherein said cobalt(II) complex of the metallizable dye is capable of reacting with said cobalt(III) complex to produce a cobalt(III) complex of the metallizable dye and the cobalt(II) complex; and (3) a water soluble polymer.
Claim 3
Dependent Claim
30 words
a reagent composition according to claim 1 wherein said water soluble polymer includes a repeating unit having a group selected from the group consisting of acrylamide, vinylpyrrolidone, acrylate and vinylalcohol.
Claim 4
Dependent Claim
15 words
A reagent composition according to claim 2 wherein said repeating unit is vinylpyrrolidone or acrylamide.
Claim 5
Dependent Claim
17 words
A reagent composition according to claim 3 wherein said water soluble polymer is poly(acrylamide-co-N-vinyl-2-pyrrolidone) (90:10 by weight).
Claim 6
Dependent Claim
14 words
A reagent composition according to any previous claim, further comprising an electron transfer agent.
Claim 7
Dependent Claim
15 words
A reagent composition according to claim 5 wherein said electron transfer agent is a quinone.
Claim 8
Dependent Claim
21 words
A reagent composition according to claim 1 comprising (1) [Co(ethylenediamine)₂ (2,2ʹbipyri-dine)]Cl₃. (2) 2-[(5-carboxy-2-pyridyl)azo]-1-naphthol-4-sulfonic acid, diammonium salt (3) poly(acrylamide-co-N-vinyl-2-pyrrolidone) (90:10 by weight).
Claim 9
Dependent Claim
15 words
An element comprising a support having coated thereon a reagent composition according to claim 1.
Claim 10
Dependent Claim
34 words
An element according to claim 8 wherein the water soluble dye is coated on one side of the support and the remainder of the reagents are coated on the other side of the support.
Claim 11
Independent Claim
129 words
A method for the determination of an analyte in a liquid comprising the steps of: (A) contacting a sample of said liquid with a reagent composition comprising: (1) a water soluble cobalt(III) complex which is capable of being reduced to a water soluble cobalt(II) complex by said analyte and, (2) a water soluble dye which is capable of being metallized by said cobalt(II) complex to form a water soluble cobalt(II) complex of the metallizable dye, wherein said cobalt(II) complex of the metallizable dye is capable of reacting with said cobalt(III) complex to produce a cobalt(III) complex of the metallizable dye and the cobalt(II) complex; and (3) a water soluble polymer so a to produce a detectable change in the presence of said analyte and (B) detecting said detectable change.
Claim 12
Independent Claim
129 words
A method for the determination of an analyte in a liquid comprising the steps of: (A) contacting a sample of said liquid with a reagent composition comprising: (1) a water soluble cobalt(III) complex which is capable of being reduced to a water soluble cobalt(II) complex by said analyte and, (2) a water soluble dye which is capable of being metallized by said cobalt(II) complex to form a water soluble cobalt(II) complex of the metallizable dye, wherein said cobalt(II) complex of the metallizable dye is capable of reacting with said cobalt(III) complex to produce a cobalt(III) complex of the metallizable dye and the cobalt(II) complex; and (3) a water soluble polymer so a to produce a detectable change in the presence of said analyte and (B) detecting said detectable change.
References Cited (Backward Citations)
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