Microorganisms for degrading toxic waste materials

Claim 2

The microorganism Pseudomonas putida strain UNK-1 and mutations thereof, said microorganism being further identified by accession number IVI-10112 and being capable of degrading haloaromatic compounds in a liquid medium.

Claim 3

The facultatively anaerobic microorganism Klebsiella oxytoca strain SAL-18A and mutations thereof, said microorganism being further identified by accession number IVI-10113, said microorganism being capable of degrading haloaromatic compounds in a solid medium.

Claim 4

The facultatively anaerobic microorganism Klebsiella oxytoca strain SAL-18A and mutations thereof, said microorganism being further identified by accession number IVI-10113, said microorganism being capable of degrading haloaromatic compounds in a solid medium.

Claim 5

A process for the microbial degradation of toxic halogenated organic chemical waste into less toxic materials, which comprises applying to the locus of said halogenated organic chemical waste the microorganism Pseudomonas putida strain UNK-1 and mutations thereof, said microorganism being further identified by accession number IVI-­10112, and monitoring the removal of contaminants from the waste locus.

Claim 6

A process for the microbial degradation of toxic halogenated organic chemical waste into less toxic materials, which comprises applying to the locus of said halogenated organic chemical waste the microorganism Pseudomonas putida strain UNK-1 and mutations thereof, said microorganism being further identified by accession number IVI-­10112, and monitoring the removal of contaminants from the waste locus.

Claim 7

The process of Claim 3 wherein the locus of the waste to be degraded is soil containing halogenated organic chemical waste, the soil being cultivated in the presence of oxygen or air with the microorganism and combined with a nutrient.

Claim 8

A process for the microbial degradation of toxic halogenated organic chemical waste into less toxic materials, which comprises applying to the locus of said halogenated organic chemical waste the microorganism Klebsiella oxytoca strain SAL-18A and mutations thereof, said microorganism being further identified by accession number IVI-10113, and monitoring the removal of contaminants from the waste locus.

Claim 9

A process for the microbial degradation of toxic halogenated organic chemical waste into less toxic materials, which comprises applying to the locus of said halogenated organic chemical waste the microorganism Klebsiella oxytoca strain SAL-18A and mutations thereof, said microorganism being further identified by accession number IVI-10113, and monitoring the removal of contaminants from the waste locus.

Claim 10

The process of Claim 5 wherein the locus of the waste to be degraded is soil containing halogenated organic chemical waste, the soil being cultivated in the presence or absence of oxygen or air with the microorganism and combined with a nutrient.

Claim 11

The process of any one of Claims 4 and 6 wherein the locus to be degraded is a landfill.

Claim 12

The process of any one of Claims 4 and 6 wherein the locus to be degraded is a leachate removed from the landfill.

Claim 13

The process of any one of Claims 3 and 5 wherein the halogenated or­ganic chemicals are predominantly chlorinated aromatic chemicals.

Claim 14

The process of Claim 9 wherein the metabolic pathway for degradation includes one or more chloro benzoic acid compounds.

Claim 15

A process for biologically treating chemical process wastewater and chemical waste landfill leachate in a sequencing batch reactor system to degrade the recalcitrant organic compounds contained therein comprising the steps of: a) establishing an activated sludge in the sequencing batch reactor containing a liquid medium having both recalcitrant and non-recalcitrant organic compounds, said activated sludge being capable of metabolizing the non-recalcitrant components of the wastewater or leachate, b) augmenting the activated sludge with a microorganism capable of metabolizing the recalcitrant organic compounds, said microorganism being identified as Pseudomonas putida strain UNK-1, and mutations thereof, said microorganism being further identified by accession number IVI-10112. c) forming a stable biomass in the reactor capable of biodegrading the recalcitrant and non-recalcitrant compo­nents of the chemical process wastewater or leachate, and d) disposing of the waste effluent and waste sludge produced in the reactor.

Claim 16

A process for biologically treating chemical process wastewater and chemical waste landfill leachate in a sequencing batch reactor system to degrade the recalcitrant organic compounds contained therein comprising the steps of: a) establishing an activated sludge in the sequencing batch reactor containing a liquid medium having both recalcitrant and non-recalcitrant organic compounds, said activated sludge being capable of metabolizing the non-recalcitrant components of the wastewater or leachate, b) augmenting the activated sludge with a microorganism capable of metabolizing the recalcitrant organic compounds, said microorganism being identified as Pseudomonas putida strain UNK-1, and mutations thereof, said microorganism being further identified by accession number IVI-10112. c) forming a stable biomass in the reactor capable of biodegrading the recalcitrant and non-recalcitrant compo­nents of the chemical process wastewater or leachate, and d) disposing of the waste effluent and waste sludge produced in the reactor.

Claim 17

A process for biologically treating chemical process wastewater and chemical waste landfill leachate in a sequencing batch reactor system to degrade the recalcitrant organic compounds contained therein comprising the steps of: a) establishing an activated sludge in the sequencing batch reactor containing a liquid medium having both recalcitrant and non-recalcitrant organic compounds, said activated sludge being capable of metabolizing the non-recalcitrant components of the wastewater or leachate, b) augmenting the activated sludge with a microorganism capable of metabolizing the recalcitrant organic compounds, said microorganism being identified as Klebsiella oxytoca strain SAL-18A and mutations thereof, said microorganism being further indentified by accession number ICI-10113, c) forming a stable biomass in the reactor capable of biodegrading the recalcitrant and non-recalcitrant components of the chemical process wastewater or leachate, and d) disposing of the waste effluent and waste sludge produced in the reactor.

Claim 18

A process for biologically treating chemical process wastewater and chemical waste landfill leachate in a sequencing batch reactor system to degrade the recalcitrant organic compounds contained therein comprising the steps of: a) establishing an activated sludge in the sequencing batch reactor containing a liquid medium having both recalcitrant and non-recalcitrant organic compounds, said activated sludge being capable of metabolizing the non-recalcitrant components of the wastewater or leachate, b) augmenting the activated sludge with a microorganism capable of metabolizing the recalcitrant organic compounds, said microorganism being identified as Klebsiella oxytoca strain SAL-18A and mutations thereof, said microorganism being further indentified by accession number ICI-10113, c) forming a stable biomass in the reactor capable of biodegrading the recalcitrant and non-recalcitrant components of the chemical process wastewater or leachate, and d) disposing of the waste effluent and waste sludge produced in the reactor.

Claim 19

The process of any one of Claims 11 and 12 wherein the re­calcitrant compounds are chlorinated aromatic compounds.

Claim 20

The process of Claim 13 wherein the chlorinated aromatic compounds are chlorobenzoic acids.