Patent EP0248656A2 successfully refreshed from Google Patents.
EP0248656A2
Expired / Lapsed
EP
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Composition for cell cultivation and use thereof
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1987-06-03
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Granted: —
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Expires: 2007-06-03
Abstract
Abstract A composition for cultivating an animal cell which contains one or more of polyethylene glycol, polyvinyl alcohol and polypropylene glycol and in addition, if desired, a low density lipoprotein. The invention also relates to a process for cultivating an animal cell, comprising cultivating an animal cell in a medium for cultivating the animal cell which medium contains one or more of polyethylene glycol, polyvinyl alcohol and polypropylene glycol and in addition, if desired, a low density lipoprotein; and the invention further relates to process for producing a biologically active substance producing animal cell in a medium for cultivating the animal cell which medium contains one or more of polyethylene glycol, polyvinyl alcohol and polypropylene glycol and in addition, if desired, a low density lipoprotein, producing and accumulating the biologically active substance in the culture medium and collecting the biologically active substance.
Inventors
- 👤 Kazuaki Kitano
- 👤 Yasushi Shintani
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 23:42
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; C12N5/0037; C12N2500/00; C12N2500/50
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
29 words
(1) A composition for cultivating animal cell which contains one or more of polyethylene glycol, polyvinyl alcohol and polypropylene glycol and in addition, if desired, a low density lipoprotein.
Claim 2
Dependent Claim
13 words
(2) A composition according to Claim 1, wherein said composition contains polyethylene glycol.
Claim 3
Dependent Claim
21 words
(3) A composition according to Claim 1, wherein said composition contains polyethylene glycol and/or polyvinyl alcohol and a low density lipoprotein.
Claim 4
Dependent Claim
15 words
(4) A composition according to Claim 1, 2 or 3, wherein said composition is solid.
Claim 5
Dependent Claim
15 words
(5) A composition according to Claim 1, 2 or 3, wherein said composition is solution.
Claim 6
Dependent Claim
18 words
(6) A composition according to Claim 5, wherein the concentration of polyethylene glycol is about 10% or less.
Claim 7
Dependent Claim
13 words
(7) A composition according to Claim 6, wherein said composition contains no serum.
Claim 8
Dependent Claim
20 words
(8) A composition according to Claim 6, wherein said composition contains serum in an amount less than usual amount used.
Claim 9
Dependent Claim
43 words
(9) A process for cultivating animal cell, comprising cultivating an animal cell in a medium for cultivating the animal cell which medium contains one or more of polyethylene glycol, polyvinyl alcohol and polypropylene glycol and in addition, if desired, a low density lipoprotein.
Claim 10
Dependent Claim
26 words
(10) A process for cultivating animal cell according to Claim 9, wherein the medium for cultivating the animal cell is a medium which contains polyethylene glycol.
Claim 11
Dependent Claim
34 words
(11) A process for cultivating animal cell according to Claim 9, wherein the medium for cultivating the animal cell is a medium which contains polyethylene glycol and/or polyvinyl alcohol and a low density lipoprotein.
Claim 12
Dependent Claim
33 words
(12) A process for cultivating animal cell according to Claim 9, 10 or 11, wherein the medium for cultivating the animal cell is a medium which contains 10% or less of polyethylene glycol.
Claim 13
Dependent Claim
30 words
(13) A process for cultivating animal cell according to Claim 9, 10, 11 or 12, wherein the medium for cultivating the animal cell is a medium which contains no serum.
Claim 14
Dependent Claim
37 words
(14) A process for cultivating animal cell according to Claim 9, 10, 11 or 12 wherein the medium for cultivating the animal cell is a medium which contains serum in an amount less than usual amount used.
Claim 15
Dependent Claim
61 words
(15) A process for producing an biologically active substance, comprising cultivating a biologically active substance producing animal cell in a medium for cultivating the animal cell which medium contains one or more of polyethylene glycol, polyvinyl alcohol and polypropylene glycol and in addition, if desired, a low density lipoprotein, producing and accumulating said substance in the culture and collecting the substance.
Claim 16
Dependent Claim
23 words
(16) A production process according to Claim 15, wherein the medium for cultivating the animal cell is a medium which contains polyethylene glycol.
Claim 17
Dependent Claim
31 words
(17) A production process according to Claim 15, wherein the medium for cultivating the animal cell is a medium which contains polyethylene glycol and/or polyvinyl alcohol and a low density lipoprotein.
Claim 18
Dependent Claim
30 words
(18) A production process according to Claim 15, 16 or 17, wherein the medium for cultivating the animal cell is a medium which contains 10% or less of polyethylene glycol.
Claim 19
Dependent Claim
27 words
(19) A production process according to Claim 15, 16 17 or 18, wherein the medium for cultivating the animal cell is a medium which contains no serum.
Claim 20
Dependent Claim
34 words
(20) A production process according to Claim 15, 16, 17 or 18, wherein the medium for cultivating the animal cell is a medium which contains serum in an amount less than usual amount used.
References Cited (Backward Citations)
No citation records indexed.
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