Patent EP0244234A2 successfully refreshed from Google Patents.
EP0244234A2
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EP
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Transformation of trichoderma
Assignee: ALTIA GROUP Ltd
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Filing: 1987-04-29
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Granted: —
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Expires: 2007-04-29
Abstract
Abstract A vector system for transformation of Trichoderma has been developed. The vector system can be used for high expression and secretion of proteins in Trichoderma by transformation of a suitable Trichoderma strain with the vector system comprising a gene for a desired protein.
Inventors
- 👤 Jonathan Knowles
- 👤 Anu Marjukka Harkki
- 👤 Helena Nevalainen
- 👤 Merja Penttilä
- 👤 Mogens Trier Hansen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 14:49
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C12N9/2428; C07; C07K; C07K14/00; C07K14/37; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/87; C12N15/90; C12N9/2434; C12N9/2437; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6483; C12N9/78; C12N9/80; C12Y; C12Y302/00; C12Y302/01; C12Y302/01003; C12Y302/01004; C12Y302/01091; C12Y304/00; C12Y304/23; C12Y304/23004; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
40 words
A vector system for use in the transformation of Trichoderma comprising a) a gene encoding a desired protein product, b) functions facilitating gene expression including promoters/enhancers operably linked to control expression of the desired product, and c) a selection marker.
Claim 2
Dependent Claim
40 words
A vector system for use in the transformation of Trichoderma comprising a) a gene encoding a desired protein product, b) functions facilitating gene expression including promoters/enhancers operably linked to control expression of the desired product, and c) a selection marker.
Claim 3
Dependent Claim
25 words
A vector system according to claim 1 further comprising a signal/leader sequence fused upstream to the 5'end of the gene for the desired protein product.
Claim 4
Dependent Claim
16 words
A vector system according to claim 1 or 2, wherein the promoter can function in Trichoderma.
Claim 5
Dependent Claim
20 words
A vector system according to claim 1, wherein the promoter is derived from a gene for protein heterologous to Trichoderma.
Claim 6
Dependent Claim
22 words
A vector system according to claim 2, wherein the signal/leader sequence is derived from a gene for a protein secreted by Trichoderma.
Claim 7
Dependent Claim
20 words
A vector system according to claim 2, wherein the signal/leader sequence is derived from a secreted protein heterologous to Trichoderma.
Claim 8
Dependent Claim
23 words
A vector system according to claim 5 to 6, wherein the signal/leader sequence is derived from the signal/leader sequence of the desired proteins.
Claim 9
Dependent Claim
18 words
A vector system according to claim 5, wherein the signal sequence is the Trichoderma reesei cbhl signal sequence.
Claim 10
Dependent Claim
17 words
A vector system according to claim 6, wherein the signal sequence is the Aspergillus glucoamylase signal sequence.
Claim 11
Dependent Claim
15 words
A vector system according to claim 1, wherein the desired protein is homologous to Trichodersa.
Claim 12
Dependent Claim
15 words
A vector system according to claim 1, wherein the desired protein is heterologous to Trichoderma.
Claim 13
Dependent Claim
13 words
A vector system according to claim 11, wherein the desired product is prochymosin.
Claim 14
Dependent Claim
17 words
A vector system according to claim 10, wherein the desired protein is T. reesei endoglucanase (EG I).
Claim 15
Dependent Claim
16 words
A vector system according to claim 3, wherein the promoter is the Trichoderma reesei cbhl promoter.
Claim 16
Dependent Claim
15 words
A vector system according to claim 4, wherein the promoter is the Aspergillus glucoamylase promoter.
Claim 17
Dependent Claim
36 words
A vectpr system according to claim 1, wherein the selection marker is derived from the Aspergillus nidulans amdS gene, the Aspergillus nidulans or Trichoderma reesei argB gene or the Neurospora crassa or Trichoderma reesei pyr4 gene.
Claim 18
Dependent Claim
14 words
A vector system according to claim 1 comprising at least two plasmids or vectors.
Claim 19
Dependent Claim
32 words
A vector system according to claim 17, wherein the selection marker is situated on one plasmid and the remaining DNA-sequences to be incorporated in the host genome are situated on another plasmid.
Claim 20
Dependent Claim
17 words
Trichoderma strains stably transformed with a vector system in accordance with any of the previous claims 1-18.
Claim 21
Independent Claim
26 words
A method for transformation of Trichoderma, wherein a suitable Trichoderma strain is transformed with a vector system in accordance with any of the previous claims 1-18.
Claim 22
Independent Claim
53 words
A method for the production of a protein product in Trichoderma, wherein a suitable host microorganism is transformed with a vector system in accordance with any one of claims 1-18, the transformed strain is cultured in a suitable culture medium and the expressed and secreted protein product is recovered from the culture medium.
Claim 23
Independent Claim
48 words
A method for the production of a protein product in Trichoderma which comprises cultivating in a suitable culture medium a suitable host microorganism transformed with a vector system in accordance with any one of claims 1-18 and recovering the expressed and secreted protein product from the culture medium.
Claim 24
Independent Claim
19 words
A method in accordance with claims 21 or 22, wherein the host microorganism is a prototrophic T. reesei strain.
Claim 25
Dependent Claim
19 words
A method in accordance with claim 21 or 22, wherein the host microorganism is an auxotrophic T. reesei strain.
Claim 26
Independent Claim
22 words
A method in accordance with anyone of claims 21-24, wherein the host strain is deficient to any gene encoding an undesired product.
Claim 27
Dependent Claim
16 words
A method according to claim 25, wherein the host strain is deficient in the cbhl gene.
Claim 28
Dependent Claim
23 words
A u xotrophic Trichoderma strains usable as host strains to be transformed with the vector system in accordance with any of claims 1-18.
References Cited (Backward Citations)
No citation records indexed.
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