Patent EP0241940A2 successfully refreshed from Google Patents.
EP0241940A2
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Method of forming a capillary element for use in an electrophoresis instrument
Assignee: Applied Biosystems Inc
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Filing: 1987-04-16
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Granted: —
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Expires: 2007-04-16
Abstract
Abstract The basic concept of this invention involves the use of a wire or capillary tube as a template strand. The outside diameter and shape of the template strand correspond to the inside diameter and shape of a desired separation capillary. A detector may be incorporated by providing a pair of electrode wires, or the ends of a pair of optical fibers, and pressing them against opposite sides of the template. A plastic is then polymerized around this assembly by casting or molding. The template is then removed, leaving a capillary channel with a sidewall incorporating the wire electrodes and optical fibers. Using this method, it is possible to form a single unit including a separation capillary, conductivity detector, and spectroscopy detector.
Inventors
- 👤 Kenneth Ogan
- 👤 Frans M. Everaerts
- 👤 Theo P.E.M. Verheggen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 17:14
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44756
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
57 words
The method of forming a capillary component for use in an electrophoresis instrument which comprises: providing a template strand having external profile dimensions and shape corresponding to the internal profile dimensions and shape of a desired capillary component; encasing said template strand within a body of hardenable material; hardening said hardenable material; and removing said template strand.
Claim 2
Independent Claim
57 words
The method of forming a capillary component for use in an electrophoresis instrument which comprises: providing a template strand having external profile dimensions and shape corresponding to the internal profile dimensions and shape of a desired capillary component; encasing said template strand within a body of hardenable material; hardening said hardenable material; and removing said template strand.
Claim 3
Dependent Claim
43 words
The method of claim 1 including, prior to encasing, the steps of: contacting one portion of said strand with the end of a light transmitting member; and contacting another portion of said strand with an optically opaque member defining an optical aperture therethrough.
Claim 4
Dependent Claim
61 words
The method of claim 1 including, prior to encasing, the additional steps of: contacting a first point on the surface of said template strand with a first fiber capable of conducting a component-detecting medium; and contacting a second point on the surface of said template strand, substantially opposite said first point, with a second fiber capable of conducting said component-detecting medium.
Claim 5
Dependent Claim
19 words
The method of claim 3 wherein said first and second fibers are wires and said component-detecting medium is electricity.
Claim 6
Dependent Claim
21 words
The method of claim 3 wherein said first and second fibers are optical fibers and said component-detecting medium is electromagnetic radiation.
Claim 7
Dependent Claim
33 words
The method of claim 4 wherein each of said contacting steps comprises: wrapping said wire over the end of an applicator member; and pressing said applicator member and wire against the template strand.
Claim 8
Dependent Claim
45 words
The method of claim 4 wherein each of said contacting steps comprises: looping said wire over said template strand and exerting a pulling force on said wire to make intimate contact between said wire and strand over substantially one half the circumference of said strand.
Claim 9
Dependent Claim
20 words
The method of claim 5 wherein each of said optical fibers includes a polished end making contact with said strand.
Claim 10
Dependent Claim
12 words
The method of claim 1 wherein said strand is a solid wire.
Claim 11
Dependent Claim
12 words
The method of claim 1 wherein said strand is a capillary tube.
Claim 12
Dependent Claim
18 words
The method of claim 1 wherein said removing step comprises pulling the template strand from the hardened material.
Claim 13
Dependent Claim
18 words
The method of claim 1 wherein said removing step comprises dissolution of the material of said template strand.
Claim 14
Dependent Claim
46 words
The method of claim 1 wherein, prior to the encasing step, the end of a capillary tube is slid over said template strand in close proximity thereto and extending a preselected distance therealong; and said capillary tube is removed after the hardening of said hardenable material.
Claim 15
Dependent Claim
94 words
A detector for use in capillary isotachophoresis which comprises: a body of hardened plastic material defining therethrough a separation capillary; a first fiber capable of conducting a component-detecting medium molded into said body with a portion of its surface forming a first region of the surface of the separation capillary; and a second fiber capable of conducting a component-detecting medium molded into said body with a portion of its surface forming a second region of the surface of the separation capillary, said first and second regions being on opposite sides of said separation capillary.
Claim 16
Dependent Claim
94 words
A detector for use in capillary isotachophoresis which comprises: a body of hardened plastic material defining therethrough a separation capillary; a first fiber capable of conducting a component-detecting medium molded into said body with a portion of its surface forming a first region of the surface of the separation capillary; and a second fiber capable of conducting a component-detecting medium molded into said body with a portion of its surface forming a second region of the surface of the separation capillary, said first and second regions being on opposite sides of said separation capillary.
Claim 17
Dependent Claim
19 words
The detector of claim 14 wherein said first and second fibers are wires and said component-detecting medium is electricity.
Claim 18
Dependent Claim
25 words
The detector of claim 15 wherein both of said first and second regions are in a plane normal to the axis of said separation capillary.
Claim 19
Dependent Claim
21 words
The detector of claim 14 wherein said first and second fibers are optical fibers and said component-detecting medium is electromagnetic radiation.
Claim 20
Dependent Claim
79 words
A capillary component for use in an electrophoresis instrument which comprises: a body of hardened plastic material defining therethrough a separation capillary; first and second electrically conductive wires molded into said body with portions of each wire forming spaced first and second regions of the surface of the separation capillary; and first and second optical fibers molded into said body with one end of each fiber forming spaced third and fourth regions of the surface of the separation capillary.
Claim 21
Dependent Claim
79 words
A capillary component for use in an electrophoresis instrument which comprises: a body of hardened plastic material defining therethrough a separation capillary; first and second electrically conductive wires molded into said body with portions of each wire forming spaced first and second regions of the surface of the separation capillary; and first and second optical fibers molded into said body with one end of each fiber forming spaced third and fourth regions of the surface of the separation capillary.
Claim 22
Dependent Claim
27 words
The component of claim 18 wherein said separation capillary includes an enlarged diameter portion axially displaced from all of said first, second, third, and fourth surface regions.
References Cited (Backward Citations)
No citation records indexed.
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