Patent EP0240208B1 successfully refreshed from Google Patents.
EP0240208B1
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EP
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Anti-sense regulation of gene expression in plant cells
Assignee: Monsanto Co
•
Filing: 1987-03-19
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Granted: 1994-11-17
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Expires: 2007-03-19
Abstract
No abstract record available.
Inventors
- 👤 Christine K. Shewmaker
- 👤 Jean C. Kridl
- 👤 William R. Hiatt
- 👤 Vic Knauf
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 13:28
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1137; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8218; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12Y; C12Y302/00; C12Y302/01; C12Y302/01015; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; Y; Y02; Y02A; Y02A40/00; Y02A40/10; Y02A40/146
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
35 words
A recombinant DNA construct, comprising a polygalacturonase (PG) DNA sequence as found in the EcoRI insert of plasmid pCGN1401 (ATCC 67227), or as obtainable by the use of said insert DNA as a hybridization probe.
Claim 2
Dependent Claim
19 words
A recombinant DNA construct according to claim 1, wherein said DNA sequence comprises an open reading frame encoding PG.
Claim 3
Dependent Claim
24 words
A recombinant DNA construct according to claim 1 or claim 2 wherein said DNA sequence is a cDNA sequence derived from the PG gene.
Claim 4
Dependent Claim
19 words
A recombinant DNA construct according to any one of the preceding claims, which comprises also a prokaryotic replication system.
Claim 5
Dependent Claim
28 words
A recombinant DNA construct according to any one of the preceding claims including a marker for selection of a eukaryotic cell into which said construct has been inserted.
Claim 6
Dependent Claim
59 words
A recombinant DNA construct according to any one of the preceding claims wherein a sequence of at least 15 base pairs of PG DNA is joined, in the opposite orientation to normal for expression, to a transcription initiation region functional in plants, whereby an anti-sense PG RNA sequence will be transcribed in a plant cell operatively carrying said construct.
Claim 7
Dependent Claim
24 words
A recombinant DNA construct according to claim 6 wherein said DNA sequence comprises a strand complementary to at least 200 nucleotides of PG mRNA.
Claim 8
Dependent Claim
27 words
A recombinant DNA construct according to claim 6 or claim 7 including a transcription termination region functional in plants and located 3' to said PG DNA sequence.
Claim 9
Independent Claim
60 words
A method for regulating the expression of a gene in a plant cell which comprises: integrating into said plant cell genome a recombinant DNA construct of any one of claims 6, 7 and 8; growing said plant cell containing said integrated construct, whereby said antisense RNA is transcribed and modulates the expression of PG in RNA in said plant cell.
Claim 10
Dependent Claim
11 words
A plant cell as obtainable by the method of claim 9.
Claim 11
Dependent Claim
14 words
A plant derived from a cell as obtainable by the method of claim 9.
Claim 12
Dependent Claim
5 words
The plasmid pCGN1401 (ATCC 67227).
References Cited (Backward Citations)
No citation records indexed.
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