EP0239222A1
Expired / Lapsed
EP
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Analytical element containing photosensitive compound and filter layer and method of use
Assignee: Eastman Kodak Co
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Filing: 1987-02-17
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Granted: —
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Expires: 2007-02-17
Abstract
Abstract A multilayer analytical element for the determination of a clinically significant enzyme analyte comprises a photosensitive compound (for example, a photosensitive dye or dye precursor) and a filter layer containing one or more filter dyes. The filter layer is situated in the element such that incident radiation used to detect a density change resulting from interaction of the analyte and the photosensitive compound passes through the filter layer before it reaches the photosensitive compound. The use of the filter layer inhibits premature changes in the photosensitive compound caused by incident radiation. This element is particu- lady useful for the determination of creatine kinase or one of its isoenzymes, for example, creatine kinase-MB.
Inventors
- 👤 John Bruce Findlay
- 👤 Brent Arthur Burdick
- 👤 Ronald Andrew Wellman
- 👤 Mark Eldon Shafer
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 09:57
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/50; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
67 words
A self-supporting analytical element comprising an absorbent carrier material containing an interactive composition for a clinically significant enzyme analyte comprising a photosensitive compound, characterized in that the element has a filter layer comprising at least one filter dye and situated in relation to the carrier material such that incident radiation for detecting the density change passes through the filter layer prior to incidence upon the photosensitive compound.
Claim 2
Dependent Claim
67 words
A self-supporting analytical element comprising an absorbent carrier material containing an interactive composition for a clinically significant enzyme analyte comprising a photosensitive compound, characterized in that the element has a filter layer comprising at least one filter dye and situated in relation to the carrier material such that incident radiation for detecting the density change passes through the filter layer prior to incidence upon the photosensitive compound.
Claim 3
Dependent Claim
114 words
A multilayer analytical element containing an interactive composition comprising a photosensitive dye or dye precursor which is capable of providing an optical density change as a result of interaction with a clinically significant enzyme analyte, the element comprising a nonporous support having thereon one or more layers, one of which contains the photosensitive dye or dye precursor, characterized in that the element further comprises a filter layer comprising at least one filter dye and situated in relation to the layer containing the photosensitive dye or dye precursor such that incident radiation for detecting the density change passes through the filter layer prior to incidence upon the layer containing the photosensitive dye or dye precursor.
Claim 4
Dependent Claim
114 words
A multilayer analytical element containing an interactive composition comprising a photosensitive dye or dye precursor which is capable of providing an optical density change as a result of interaction with a clinically significant enzyme analyte, the element comprising a nonporous support having thereon one or more layers, one of which contains the photosensitive dye or dye precursor, characterized in that the element further comprises a filter layer comprising at least one filter dye and situated in relation to the layer containing the photosensitive dye or dye precursor such that incident radiation for detecting the density change passes through the filter layer prior to incidence upon the layer containing the photosensitive dye or dye precursor.
Claim 5
Dependent Claim
38 words
The element as claimed in claim 2 wherein the support is transparent to incident radiation and the photosensitive dye or dye precursor is in a layer located on the opposite side of the support from the filter layer.
Claim 6
Dependent Claim
26 words
The element as claimed in claim 2 wherein the filter layer is located between the support and a layer containing the photosensitive dye or dye precursor.
Claim 7
Dependent Claim
26 words
The element as claimed in any of claims 2 to 4 wherein the interactive composition provides a detectable optical density change in response to creatine kinase.
Claim 8
Dependent Claim
21 words
The element as claimed in any of claims 2 to 5 wherein the photosensitive dye precursor is an imidazole leuco dye.
Claim 9
Dependent Claim
23 words
The element as claimed in any of claims 2 to 6 wherein the outermost layer on the support is a porous spreading layer.
Claim 10
Dependent Claim
33 words
The element as claimed in claim 7 wherein the interactive composition comprises adenosine-5'diphosphate, glycerol, glycerol kinase and a-glycerophosphate oxidase and the element further comprises creatine kinase-M antibodies immobilized in the porous spreading layer.
Claim 11
Independent Claim
57 words
A method for the determination of a clinically significant enzyme analyte comprising the steps of: A. contacting a sample of a liquid suspected of containing a clinically significant enzyme analyte with an analytical element as claimed in any of claims 1 to 8, and B. determining the optical density change resulting from the presence of the analyte.
Claim 12
Dependent Claim
23 words
The method as claimed in claim 9 wherein the detectable optical density change is determined at a wavelength of greater than 500 nm.
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