EP0116307A2
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EP
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Composition, analytical element and method for the quantification of creatine kinase
Assignee: Eastman Kodak Co
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Filing: 1984-01-16
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Granted: —
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Expires: 2004-01-16
Abstract
Abstract A method is described forthe analytical determination of creatine kinase in an aqueous liquid, such as blood serum. This method comprises: (A) contacting (1) a sample of the liquid to be analyzed, and (2) reagents which effect in the presence of a creatine kinase-containing liquid, an ordered sequence of reactions wherein: (a) creatine phosphate and adenosine diphosphate react in the presence of creatine kinase to form creatine and adenosine triphosphate; (b) glycerol is phosphorylated in the presence of glycerol kinase and adenosine triphosphate to form L-a-glycerophosphate; and (c) L-a-glycerophosphate is oxidized in the presence of a-glycerophosphate oxidase and a colorimetric indicator composition to produce an optical density change; and (B) quantitatively detecting the rate of such change. This method can be practiced in solution or on "dry" analytical elements containing a plurality of zones.
Inventors
- 👤 Theodore W. Esders
- 👤 Shirley Y. Lynn
- 👤 John B. Findlay
- 👤 Richard M. Schubert
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 20:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/26; C12Q1/28; C12Q1/50; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
111 words
A method for the quantification of creatine kinase in an aqueous liquid, said method comprising the steps of: (A) contacting (1) a sample of said liquid, and (2) reagents which effect in the presence of a creatine kinase-containing liquid, an ordered sequence of reactions wherein: (a) creatine phosphate and adenosine diphosphate react in the presence of creatine kinase to form creatine and adenosine triphosphate; (b) glycerol is phosphorylated in the presence of glycerol kinase and adenosine triphosphate to form L-a-glycerophosphate; and (c) L-a-glycerophosphate is oxidized in the presence of a-glycerophosphate oxidase and a colorimetric indicator composition to produce an optical density change; and (B) quantitatively detecting the rate of said change.
Claim 2
Independent Claim
111 words
A method for the quantification of creatine kinase in an aqueous liquid, said method comprising the steps of: (A) contacting (1) a sample of said liquid, and (2) reagents which effect in the presence of a creatine kinase-containing liquid, an ordered sequence of reactions wherein: (a) creatine phosphate and adenosine diphosphate react in the presence of creatine kinase to form creatine and adenosine triphosphate; (b) glycerol is phosphorylated in the presence of glycerol kinase and adenosine triphosphate to form L-a-glycerophosphate; and (c) L-a-glycerophosphate is oxidized in the presence of a-glycerophosphate oxidase and a colorimetric indicator composition to produce an optical density change; and (B) quantitatively detecting the rate of said change.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein said colorimetric indicator composition comprises a leuco dye and a substance having peroxidative activity.
Claim 4
Dependent Claim
15 words
The method of claim 1 wherein said aqueous medium further comprises a creatine kinase activator.
Claim 5
Dependent Claim
22 words
The method of claim 3 wherein said creatine kinase activator is added to said sample prior to contacting with the reagent composition.
Claim 6
Dependent Claim
14 words
The method of claim 3 or 4 wherein said creatine kinase activator is N-acetylcysteine.
Claim 7
Dependent Claim
26 words
The method of claim 3, 4 or 5 wherein said activator is present in said aqueous medium in a concentration between 0.05 mM and 0.15 mM.
Claim 8
Independent Claim
19 words
The method of any previous claim wherein said aqueous medium is buffered to a pH between 6 and 9.
Claim 9
Dependent Claim
35 words
A composition for the quantification of creatine kinase in an aqueous liquid, said composition comprising: (a) creatine phosphate; (b) adenosine diphosphate; (c) glycerol ; (d) glycerol kinase; (e) a-glycerophosphate oxidase; (f) a colorimetric indicator composition.
Claim 10
Dependent Claim
35 words
A composition for the quantification of creatine kinase in an aqueous liquid, said composition comprising: (a) creatine phosphate; (b) adenosine diphosphate; (c) glycerol ; (d) glycerol kinase; (e) a-glycerophosphate oxidase; (f) a colorimetric indicator composition.
Claim 11
Dependent Claim
16 words
The composition of claim 8 wherein said colorimetric indicator composition comprises a substance having peroxidative activity.
Claim 12
Dependent Claim
11 words
The composition of claim 9 wherein said peroxidative substance is peroxidase.
Claim 13
Dependent Claim
27 words
The composition of claim 9 wherein said chromogen is a material capable of undergoing a colorimetric change in the presence of a peroxide and said peroxidative substance.
Claim 14
Dependent Claim
12 words
The composition of claim 8 wherein said chromogen is a dye-forming substance.
Claim 15
Dependent Claim
12 words
The composition of claim 12 wherein said chromogen is a leuco dye.
Claim 16
Dependent Claim
29 words
The composition of claim 8 which is a solution and further comprises a creatine kinase activator present in an amount less than 0.2 mM based on final assay volume.
Claim 17
Dependent Claim
14 words
The composition of claim 14 wherein said creatine kinase activator is a mercapto-containing compound.
Claim 18
Dependent Claim
11 words
The composition of claim 15 wherein said mercapto-containing compound is N-acetylcysteine.
Claim 19
Dependent Claim
61 words
An analytical element for detecting creatine kinase, said element including a support and, in fluid contact, first and second zones; and said element comprising: creatine phosphate; adenosine diphosphate; glycerol; glycerol kinase;-a-glycerophosphate oxidase; a colorimetric indicator composition comprising a chromogen; and a creatine kinase activator, said first zone containing said colorimetric indicator composition, and said second zone containing said creatine kinase activator.
Claim 20
Dependent Claim
61 words
An analytical element for detecting creatine kinase, said element including a support and, in fluid contact, first and second zones; and said element comprising: creatine phosphate; adenosine diphosphate; glycerol; glycerol kinase;-a-glycerophosphate oxidase; a colorimetric indicator composition comprising a chromogen; and a creatine kinase activator, said first zone containing said colorimetric indicator composition, and said second zone containing said creatine kinase activator.
Claim 21
Dependent Claim
13 words
The element of claim 17 wherein said first zone is adjacent said support.
Claim 22
Dependent Claim
10 words
The element of claim 17 wherein said activator is N-acetylcysteine.
Claim 23
Dependent Claim
24 words
The element of claim 19 wherein said N-acetylcysteine is present in said second zone at a coverage of up to 2 g/m 2 .
References Cited (Backward Citations)
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