Patent US2113455A successfully refreshed from Google Patents.
Patent US2188457A successfully refreshed from Google Patents.
Patent EP0237301A2 successfully refreshed from Google Patents.
EP0237301A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Sulfonic derivatives of acylated polyethyleneimine bonded phase silica products
Assignee: Avantor Performance Materials LLC
•
Filing: 1987-03-06
•
Granted: —
•
Expires: 2007-03-06
Abstract
Abstract A bonded-phase silica product of the formula wherein Z is the backbone of a silica gel or glass; n is an integer of 6 to 40 (or has an average value in that range); and Y is a tribasic or unsaturated group of the respective formulae and wherein q is zero or one and one of R and R' is H, CH 3 or C 2 H 5 and the other is COOH, COONa, CH 2 COOH or CH 2 COONa. The products when Y is the tribasic group are useful for the purification and separation of proteins having isoelectric points below 5.
Inventors
- 👤 Hugh E. Ramsden
- 👤 David R. Nau
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 22:02
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/36; B01D15/361; B01D15/362; B01J; B01J20/00; B01J20/02; B01J20/10; B01J20/103; B01J20/22; B01J20/26; B01J20/262; B01J20/265; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28014; B01J20/28047; B01J20/28054; B01J20/28078; B01J20/281; B01J20/286; B01J20/291; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3268; B01J20/3272; B01J20/328; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
80 words
A bonded-phase silica product of the formula wherein Z is the backbone of a silica get or glass; n is an integer of 6 to 40 (or has an average value in that range); and Y is a tribasic or unsaturated group of the respective formulae and wherein q is zero or one and one of R and R' is H, CH 3 or C 2 Hs and the other is COOH, COONa, CH 2 COOH or CH 2 COONa.
Claim 2
Dependent Claim
80 words
A bonded-phase silica product of the formula wherein Z is the backbone of a silica get or glass; n is an integer of 6 to 40 (or has an average value in that range); and Y is a tribasic or unsaturated group of the respective formulae and wherein q is zero or one and one of R and R' is H, CH 3 or C 2 Hs and the other is COOH, COONa, CH 2 COOH or CH 2 COONa.
Claim 3
Dependent Claim
55 words
A product according to claim 1, wherein Z is silica gel having an average particle diameter of 3 to 70p.m and an average pore size of 5 to 100 nm, or particulate controlled pore glass having an average particle diameter of 37 to 177 µm and an average pore size of 4 to 100 nm.
Claim 4
Dependent Claim
43 words
A product according to claim 2, wherein Z is particulate silica gel having an average particle diameter of 5 to 40 µm and an average pore size of 5 to 330 nm; and n is an integer or average of 7 to 12.
Claim 5
Dependent Claim
43 words
A product according to claim 2, wherein Z is particulate silica gel having an average particle diameter of 40 to 62 µm and an average pore size of 21 to 52 nm; and n is an integer or average of 21 to 40.
Claim 6
Dependent Claim
27 words
A product according to any of claims 1 to 4, wherein q is zero, R is H or CH 3 , and R' is COOH or COONa.
Claim 7
Dependent Claim
23 words
A product according to any of claims 1 to 4, wherein q is one, R is H, and R' is COOH or COONa.
Claim 8
Dependent Claim
26 words
A Product according to any of claims 1 to 4, wherein q is zero, R is CH 2 COOH or CH 2 COONa, and R' isH.
Claim 9
Dependent Claim
18 words
A chromatographic column packed with a product according to any preceding claim when Y is the tribasic group.
Claim 10
Dependent Claim
35 words
A solid-phase extraction process for the purification and separation of protein from a sample, wherein the solid phase is a product according to any of claims 1 to 7 when Y is the tribasic group.
Claim 11
Dependent Claim
15 words
A process according to claim 9, wherein the protein has an isoelectric point below 5.
Claim 12
Dependent Claim
18 words
A process according to claim 9 or claim 10, wherein the protein is ovalbumin or bovine serum albumin.
Claim 13
Dependent Claim
90 words
A process for the preparation of a product according to any of claims 1 to 7, which comprises reacting a covalently-bound non-cross-linked poly(ethyleneimino) -propyl silica glass or poly(ethyle- neimino) propyl glass with an acylating agent of the formula R'-CH = CR-(CH 2 )q-COOH or an acid anhydride or acid halide thereof, wherein R, R' and q are as defined in claim 1; and, if desired, reacting the resultant product (when Y is the unsaturated group) with a water-soluble bisulphite, or a precursor thereof, in the presence of an oxygen source.
Claim 14
Dependent Claim
20 words
A process according to claim 12, wherein the acylating agent is maleic anhydride, and the bisulphite precursor is sodium metabisulphite.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.