Patent EP0234083A2 successfully refreshed from Google Patents.
EP0234083A2
Expired / Lapsed
EP
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Carrier for a biologically active component for immunoassay or enzymatic reaction
Assignee: Tosoh Corp
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Filing: 1986-07-04
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Granted: —
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Expires: 2006-07-04
Abstract
Abstract A carrier for a biologically active component for immunoassay or enzymatic reaction, which comprises: a) a thermoplastic resin bead having an average diameter of from 0.05 to 20 mm, b) from 1 to 25% by weight, based on the bead, of a magnetically responsive powder bonded to the bead, and c) a polymer coated thereon in a thickness of from 2 to 30 µm, said polymer having a number average molecular weight of from 200 to 10,000 and having functional groups capable of binding, or being activated to bind, the biologically active component.
Inventors
- 👤 Hiroshi Ishida
- 👤 Yuji Higo
- 👤 Masuo Inoue
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 03:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54313; G01N33/54326; G01N33/5434; G01N2446/00; G01N2446/10; G01N2446/80; G01N2446/84; G01N2446/90
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
96 words
A carrier for a biologically active component for immunoassay or enzymatic reaction, characterised in that it comprises: a thermoplastic resin bead having an average diameter of from 0.05 to 20 mm; from 1 to 25% by weight, based on the weight of the bead, of a magnetically responsive powder bound on the bead; and a polymer coated thereon to a thickness of from 2 to 30 µm, the polymer having a number average molecular weight of from 20 to 5,000 and having functional groups capable of binding, or being activated to bind, the biologically active component.
Claim 2
Dependent Claim
96 words
A carrier for a biologically active component for immunoassay or enzymatic reaction, characterised in that it comprises: a thermoplastic resin bead having an average diameter of from 0.05 to 20 mm; from 1 to 25% by weight, based on the weight of the bead, of a magnetically responsive powder bound on the bead; and a polymer coated thereon to a thickness of from 2 to 30 µm, the polymer having a number average molecular weight of from 20 to 5,000 and having functional groups capable of binding, or being activated to bind, the biologically active component.
Claim 3
Dependent Claim
21 words
A carrier as claimed in Claim 1, characterised in that the functional groups of the polymer comprise -CHO, -OH or -NH₂.
Claim 4
Dependent Claim
41 words
A carrier as claimed in Claim 1 or Claim 2, characterised in that the bead is made of a thermoplastic resin comprising a polyolefin resin and/or a vinyl resin and/or a polyester resin and/or a polyamide resin and/or a copolymer resin.
Claim 5
Dependent Claim
16 words
A carrier as claimed in any preceding claim, characterised in that the bead is substantially spherical.
Claim 6
Dependent Claim
57 words
A carrier as claimed in any preceding claim, characterised in that the magnetically responsive powder has an average particle size of from 0.01 to 10 µm and is made of iron, tri-iron tetroxide, nickel, iron-cobalt, silicon steel or a soft ferrite of the formula MFe₂O₄ wherein M is Mn, Zn, Ni, Cd, Cu, Mg, Sr or Ba.
Claim 7
Dependent Claim
55 words
A carrier as claimed in any preceding claim, characterised in that the polymer is a polymer of a monomer having the formula RCH=CRʹ-CHO wherein each of R and Rʹ is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group or a phenyl group, and having a total of from 3 to 10 carbon atoms.
Claim 8
Dependent Claim
63 words
A carrier as claimed in any of Claims 1 to 5, characterised in that the polymer is a polymer having a repeating unit of the formula: wherein each of R and Rʹ is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group or a phenyl group, and is obtained from a monomer having a total of from 6 to 12 carbon atoms.
Claim 9
Dependent Claim
71 words
A carrier as claimed in any of Claims 1 to 5, characterised in that the polymer is a polymer having -OH groups obtained by hydrolyzing a polymer having a repeating unit of the formula: wherein each of R and Rʹ is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group or a phenyl group, and is obtained from a monomer having a total of from 6 to 12 carbon atoms.
Claim 10
Dependent Claim
102 words
A carrier as claimed in any of Claims 1 to 5, characterised in that the polymer is a polymer having -NH₂ groups obtained by aminating a polymer having a repeating unit of the formula: wherein each of R and Rʹ is a hydrogen atom, an alkyl group, a halogen-substituted alkyl group of a phenyl group, and is obtained from a monomer having a total of from 6 to 12 carbon atoms, with a diamine having the formula NH₂-Rʺ-NH₂ wherein Rʺ is an alkyl group, a halogen-substituted alkyl group or a phenyl group having a total of from 2 to 12 carbon atoms.
Claim 11
Dependent Claim
32 words
A carrier as claimed in any of Claims 1 to 5, characterised in that the polymer is polyglycidyl methacrylate, a glycidyl methacrylate-tetraethyleneglycol methacrylate copolymer, an acrolein-tetraethyleneglycol methacrylate copolymer, polystyrene or polyvinyl chloride.
Claim 12
Dependent Claim
36 words
A carrier as claimed in any of Claims 1 to 5, characterised in that the functional groups of the polymer are -OH groups activated by treatment with carbodiimidazol or -NH₂ groups activated by treatment with glutaraldehyde.
Claim 13
Dependent Claim
24 words
A carrier as claimed in any preceding claim, characterised by its having bound to it a biologically active component comprising antigens, antibodies or enzymes.
Claim 14
Dependent Claim
63 words
A process for preparing a carrier as claimed in any preceding claim, characterised by depositing the magnetically responsive powder on the surface of the thermoplastic resin bead in an amount of from 1 to 25% by weight, based on the weight of the bead, and forming a layer of the polymer on the bead to a thickness of from 2 to 30 µm.
Claim 15
Dependent Claim
37 words
A process as claimed in Claim 13, characterised in that the layer of the polymer is formed by polymerizing a monomer having functional groups comprising -CHO or -OH to form a polymer having the corresponding functional groups.
Claim 16
Dependent Claim
37 words
A process as claimed in Claim 14, characterised in that the polymer has groups and is hydrolyzed to convert the groups to -OH groups or is treated with a diamine to convert the groups to -NH₂ groups.
Claim 17
Dependent Claim
22 words
A process as claimed in Claim 14, characterised in that the polymer has -OH groups and is treated with N,Nʹ-carbonyldiimidazole for activation.
Claim 18
Dependent Claim
28 words
A process as claimed in Claim 14, characterised in that the polymer has groups and is partially hydrolyzed and reacted with a silane coupling agent having -NH₂ groups.
Claim 19
Dependent Claim
22 words
A process as claimed in Claim 15, characterised in that the polymer has -NH₂ groups and is treated with glutaraldehyde for activation.
Claim 20
Dependent Claim
82 words
A process as claimed in any of Claims 13 to 18, characterised in that the thermoplastic bead is substantially spherical and is prepared by dispersing particles of a thermoplastic resin having a substantially uniform shape in a medium in which the resin is insoluble, at a temperature lower than the melting point of the resin and then heating the dispersion at a temperature of from the melting point of the resin to a temperature not higher than 30°C above the melting point.
Claim 21
Dependent Claim
25 words
A process as claimed in any of Claims 13 to 19, characterised by the additional step of treating the carrier with the biologically active component.
References Cited (Backward Citations)
No citation records indexed.
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