Patent EP0232544A2 successfully refreshed from Google Patents.
EP0232544A2
Expired / Lapsed
EP
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Process for producing physiologically active substances
Assignee: Green Cross Corp
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Filing: 1986-12-24
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Granted: —
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Expires: 2006-12-24
Abstract
Abstract A process for producing physiologically active substances, such as a plasminogen activator selected from tPA, urokinase and single-chain pro-urokinase, by recombinant DNA technology is described. In the present process, a recombinant vector DNA having a DNA sequence therein which encodes a human physiologically active substance is transfected to established cells of human kidney origin to obtain transformed cells which produce and secrete the desired physiologically active substance.
Inventors
- 👤 Teruo Kaneda
- 👤 Ken Okabayashi
- 👤 Naofumi Hayasuke
- 👤 Ryuji Hiramatsu
- 👤 Masanori Nagai
- 👤 Hirofumi Arimura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 21:35
CPC Classifications
C; C12; C12N; C12N15/00; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
56 words
A process for producing physiologically active substance comprising the steps of (l) transforming established cells of human kidney origin with a vector DNA having therein a DNA sequence which encodes a human physiologically active substance, (2) growing the resulting transformed cells, and (3) collecting said physiologically active substance which has been secreted from said transformed cells.
Claim 2
Dependent Claim
56 words
A process for producing physiologically active substance comprising the steps of (l) transforming established cells of human kidney origin with a vector DNA having therein a DNA sequence which encodes a human physiologically active substance, (2) growing the resulting transformed cells, and (3) collecting said physiologically active substance which has been secreted from said transformed cells.
Claim 3
Dependent Claim
32 words
The process for producing physiologically active substances as claimed in claim l, wherein the physiologically active substance is a plasminogen activator selected from the group consisting of tPA, urokinase and single-chain prourokinase.
Claim 4
Dependent Claim
29 words
The process for producing physiologically active substances as claimed in Claim l, wherein said vector DNA having a DNA sequence which encodes a human physiologically active substance is pSV-G₁-PreUK.
Claim 5
Dependent Claim
26 words
The process for producing physiologically active substances as claimed in Claim l, wherein said transformed cell is selected from the group consisting of WS52-ll and WS52-08.
Claim 6
Dependent Claim
19 words
The process for procuding physiologically active substances as claimed in Claim l, wherein said physiologically active substance is urokinase.
References Cited (Backward Citations)
No citation records indexed.
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