Patent EP0228779A2 successfully refreshed from Google Patents.
EP0228779A2
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EP
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Reticulated cellulose product, sheets formed therefrom, methods and microorganisms for their production
Assignee: Monsanto Co
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Filing: 1986-10-17
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Granted: —
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Expires: 2006-10-17
Abstract
Abstract Strains of Acetobacter that are stable agitated culture conditions are claimed as well as a unique reticulated cellulose product, and a method and media for producing such bacterial cellulose under agitated culture conditions. Sustained production of bacterial cellulose over an average of 70 hours of at least 0.1 g/liter per hour is achieved. Further, sheets comprising the microbially produced cellulose which are characterized by great tensile strength and by substantial resistance to densification when produced by sheet forming means, are claimed.
Inventors
- 👤 Arie Ben-Bassat
- 👤 Robert Bruner
- 👤 Sharon Payne Shoemaker
- 👤 Yehoshua Aloni
- 👤 Harry Wong
- 👤 Donald Curtis Johnson
- 👤 Amar Nath Naogi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 13:11
CPC Classifications
A; A61; A61L; A61L15/00; A61L15/16; A61L15/22; A61L15/28; C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P19/00; C12P19/04; C12R; C12R2001/00; C12R2001/01; C12R2001/02
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
23 words
A microbially produced cellulose comprising a reticulated cellulose product wherein the cellulose strands form a substantially continuous network of branching interconnected cellulose strands.
Claim 2
Dependent Claim
23 words
A microbially produced cellulose comprising a reticulated cellulose product wherein the cellulose strands form a substantially continuous network of branching interconnected cellulose strands.
Claim 3
Dependent Claim
34 words
A microbially produced reticulated cellulose product as claimed in claim 1 which demonstrates resistance to densification when formed into sheets by sheet-forming means as compared to cellulose produced in statically grown cultures of Acetobacter
Claim 4
Dependent Claim
17 words
A sheet comprising a microbially produced reticulated cellulose product as claimed in claim 1 or claim 2.
Claim 5
Dependent Claim
43 words
A sheet as claimed in claim 3 wherein said sheet is formed to a basis weight of about 60 g/m² and a thickness between about .15 and about .22 MM, and has a density ranging from about 250 kg/m³ to about 430 kg/m³.
Claim 6
Dependent Claim
39 words
A sheet as claimed in claim 3 wherein said sheet is formed to a density in a range between about 300 and about 500 kg/m³, and has a tensile index in a range between about 100 and 150 Newton-Meters/gram.
Claim 7
Independent Claim
64 words
A method of producing a cellulose product, e.g. a reticulated cellulose product, comprising incubating a cellulose producing microorganism in a liquid medium suitable for growth of said microorganism and cellulose production under agitated conditions at an average volumetric productivity of at least 0.1 g/1/hr, said average volumetric productivity being maintained over a period of at least 70 hours, and removing the cellulose product obtained.
Claim 8
Independent Claim
64 words
A method of producing a cellulose product, e.g. a reticulated cellulose product, comprising incubating a cellulose producing microorganism in a liquid medium suitable for growth of said microorganism and cellulose production under agitated conditions at an average volumetric productivity of at least 0.1 g/1/hr, said average volumetric productivity being maintained over a period of at least 70 hours, and removing the cellulose product obtained.
Claim 9
Dependent Claim
52 words
A microorganism designated Acetobacter which has the ability to produce a cellulose product under agitated culture conditions and a frequency of change to cellulose non-producing forms under agitated culture conditions as determined by the appearance of cellulose non-producing colonies on solid medium of less than 0.5% over the course of 42-45 generations.
Claim 10
Dependent Claim
52 words
A microorganism designated Acetobacter which has the ability to produce a cellulose product under agitated culture conditions and a frequency of change to cellulose non-producing forms under agitated culture conditions as determined by the appearance of cellulose non-producing colonies on solid medium of less than 0.5% over the course of 42-45 generations.
Claim 11
Dependent Claim
16 words
A microorganism designated Acetobacter which shows substantially reduced D-gluconic acid production when grown on D-glucose-containing medium.
Claim 12
Dependent Claim
16 words
A microorganism designated Acetobacter which shows substantially reduced D-gluconic acid production when grown on D-glucose-containing medium.
Claim 13
Independent Claim
94 words
A method of producing a reticulated cellulose product comprising: a) culturing a microorganism which has the ability to produce a cellulose product under agitated culture conditions in a liquid medium suitable for cellulose production at an average volumetric productivity of at least 0.1 g/1/hr over a period of time exceeding 70 hours, and a frequency of change to cellulose-non-producing forms under agitated culture conditions of less than 0.5% over the course of 42-45 generations, as determined by the appearance of cellulose non-producing colonies on solid medium, and b) removing the reticulated cellulose product obtained.
Claim 14
Independent Claim
94 words
A method of producing a reticulated cellulose product comprising: a) culturing a microorganism which has the ability to produce a cellulose product under agitated culture conditions in a liquid medium suitable for cellulose production at an average volumetric productivity of at least 0.1 g/1/hr over a period of time exceeding 70 hours, and a frequency of change to cellulose-non-producing forms under agitated culture conditions of less than 0.5% over the course of 42-45 generations, as determined by the appearance of cellulose non-producing colonies on solid medium, and b) removing the reticulated cellulose product obtained.
Claim 15
Dependent Claim
36 words
A biologically pure culture of Acetobacter xylinum having the characteristics of Acetobacter xylinum ATCC 53264, corresponding to strain 1306-3, Acetobacter xylinum ATCC 53263, corresponding to strain 1306-11, or Acetobacter xylinum ATCC 53254, corresponding to strain 1306-21.
Claim 16
Dependent Claim
36 words
A biologically pure culture of Acetobacter xylinum having the characteristics of Acetobacter xylinum ATCC 53264, corresponding to strain 1306-3, Acetobacter xylinum ATCC 53263, corresponding to strain 1306-11, or Acetobacter xylinum ATCC 53254, corresponding to strain 1306-21.
Claim 17
Dependent Claim
24 words
The production by microbial means of a reticulated cellulose product wherein the cellulose strands form a substantially continuous network of branching interconnected cellulose strands.
Claim 18
Dependent Claim
24 words
The production by microbial means of a reticulated cellulose product wherein the cellulose strands form a substantially continuous network of branching interconnected cellulose strands.
Claim 19
Dependent Claim
27 words
The production as defined in claim 11 which employs a microorganism as defined in claim 7 or claim 8 or a culture as defined in claim 10.
References Cited (Backward Citations)
No citation records indexed.
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