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EP0227459A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Arabinonucleic acid probes for DNA/RNA assays

Assignee: EIDP Inc
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Filing: 1986-12-22
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Granted: —
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Expires: 2006-12-22
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Abstract

Abstract A novel nucleic acid, arabinonucleic acid, is provided as a probe in nucleic acid assays. The arabinose moiety of the probe can be detected with anti-arabinose antibody-label conjugates.

Inventors

  • 👤 Randy Miles Mc Cormick

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-29 16:55
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804

Claims Analysis

Total Claims: 6
Claim 1
Independent Claim 2 words
Arabinonucleic acid.
Claim 2
Dependent Claim 2 words
Arabinonucleic acid.
Claim 3
Dependent Claim 26 words
The nucleic acid of Claim 1 wherein the bases of the component nucleotides are selected from the group consisting of adenine. guanine. cytosine, thyamine and uracil.
Claim 4
Independent Claim 85 words
A method for identifying nucleic acid sequences comprising the steps of: (a) rendering the target nucleic acids single-stranded: (b) immobilizing the single-stranded nucleic acids onto a support: (c) allowing said single-stranded nucleic acids to hybridize with a single-stranded arabinonucleic acid probe: (d) washing said support to remove arabinonucleic acid not incorporated into the hybrid formed on the support: and (e) determining the presence of arabinonucleic acid in the hybrid formed on the support by contacting it with an anti-arabinose antibody-label conjugate and detecting said label.
Claim 5
Independent Claim 85 words
A method for identifying nucleic acid sequences comprising the steps of: (a) rendering the target nucleic acids single-stranded: (b) immobilizing the single-stranded nucleic acids onto a support: (c) allowing said single-stranded nucleic acids to hybridize with a single-stranded arabinonucleic acid probe: (d) washing said support to remove arabinonucleic acid not incorporated into the hybrid formed on the support: and (e) determining the presence of arabinonucleic acid in the hybrid formed on the support by contacting it with an anti-arabinose antibody-label conjugate and detecting said label.
Claim 6
Dependent Claim 11 words
The method of Claim 3 wherein the label is an enzyme.

References Cited (Backward Citations)

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