EP0227459A2
Expired / Lapsed
EP
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Arabinonucleic acid probes for DNA/RNA assays
Assignee: EIDP Inc
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Filing: 1986-12-22
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Granted: —
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Expires: 2006-12-22
Abstract
Abstract A novel nucleic acid, arabinonucleic acid, is provided as a probe in nucleic acid assays. The arabinose moiety of the probe can be detected with anti-arabinose antibody-label conjugates.
Inventors
- 👤 Randy Miles Mc Cormick
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 15:47
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
2 words
Arabinonucleic acid.
Claim 2
Dependent Claim
2 words
Arabinonucleic acid.
Claim 3
Dependent Claim
26 words
The nucleic acid of Claim 1 wherein the bases of the component nucleotides are selected from the group consisting of adenine. guanine. cytosine, thyamine and uracil.
Claim 4
Independent Claim
85 words
A method for identifying nucleic acid sequences comprising the steps of: (a) rendering the target nucleic acids single-stranded: (b) immobilizing the single-stranded nucleic acids onto a support: (c) allowing said single-stranded nucleic acids to hybridize with a single-stranded arabinonucleic acid probe: (d) washing said support to remove arabinonucleic acid not incorporated into the hybrid formed on the support: and (e) determining the presence of arabinonucleic acid in the hybrid formed on the support by contacting it with an anti-arabinose antibody-label conjugate and detecting said label.
Claim 5
Independent Claim
85 words
A method for identifying nucleic acid sequences comprising the steps of: (a) rendering the target nucleic acids single-stranded: (b) immobilizing the single-stranded nucleic acids onto a support: (c) allowing said single-stranded nucleic acids to hybridize with a single-stranded arabinonucleic acid probe: (d) washing said support to remove arabinonucleic acid not incorporated into the hybrid formed on the support: and (e) determining the presence of arabinonucleic acid in the hybrid formed on the support by contacting it with an anti-arabinose antibody-label conjugate and detecting said label.
Claim 6
Dependent Claim
11 words
The method of Claim 3 wherein the label is an enzyme.
References Cited (Backward Citations)
No citation records indexed.
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